A5045252165- Gut microbiota and health
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Biochemical and biochemical processes
- Biofuel production and bioconversion
- Computational Drug Discovery Methods
- Bacteriophages and microbial interactions
- Tryptophan and brain disorders
- Antibiotic Use and Resistance
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Clostridium difficile and Clostridium perfringens research
- Microbial Natural Products and Biosynthesis
- Pneumonia and Respiratory Infections
- Chemistry and Chemical Engineering
- Neuroendocrine regulation and behavior
- Antimicrobial Peptides and Activities
- Metalloenzymes and iron-sulfur proteins
- Soil Geostatistics and Mapping
- Bioinformatics and Genomic Networks
- Bacterial Infections and Vaccines
- CRISPR and Genetic Engineering
- Microbial Community Ecology and Physiology
- Insect symbiosis and bacterial influences
University of Tübingen
2024-2025
University of Würzburg
2019-2025
European Molecular Biology Laboratory
2013-2024
TH Bingen University of Applied Sciences
2024
Czech Academy of Sciences, Institute of Microbiology
2024
Technical University of Denmark
2010-2012
Ecologie Microbienne Lyon
2009-2012
European Molecular Biology Laboratory
2012
Abstract Background Vanillin is one of the most widely used flavouring agents, originally obtained from cured seed pods vanilla orchid Vanilla planifolia . Currently vanillin mostly produced via chemical synthesis. A de novo synthetic pathway for heterologous production glucose has recently been implemented in baker's yeast, Saccharamyces cerevisiae In this study we aimed at engineering cell factory towards improved productivity and thereby developing an attractive alternative to Results...
All bactericidal antibiotics were recently proposed to kill by inducing reactive oxygen species (ROS) production, causing destabilization of iron-sulfur (Fe-S) clusters and generating Fenton chemistry. We find that the ROS response is dispensable upon treatment with antibiotics. Furthermore, we demonstrate Fe-S are required for killing only aminoglycosides. In contrast cells, using major cluster biosynthesis machinery, ISC, cells alternative SUF, cannot efficiently mature respiratory...
Drug combinations can expand options for antibacterial therapies but have not been systematically tested in Gram-positive species. We profiled ~8,000 of 65 drugs against the model species Bacillus subtilis and two prominent pathogens, Staphylococcus aureus Streptococcus pneumoniae. Thereby, we recapitulated previously known drug interactions, also identified ten times more novel interactions pathogen S. aureus, including 150 synergies. showed that synergies were equally effective...
The rise in antimicrobial resistance poses a worldwide threat, reducing the efficacy of common antibiotics. Determining activity new chemical compounds through experimental methods remains time-consuming and costly. While compound-centric deep learning models promise to accelerate this search prioritization process, current strategies require large amounts custom training data. Here, we introduce lightweight computational strategy for discovery that builds on MolE (Molecular representation...
Overproduction of a desired metabolite is often achieved via manipulation the pathway directly leading to product or through engineering distant nodes within metabolic network. Empirical examples illustrating combined effect these local and global strategies have been so far limited in eukaryotic systems. In this study, we compared effects overexpressing key gene de novo vanillin biosynthesis (coding for O-methyltransferase, hsOMT) two yeast strains, with without model-guided network...
Pharmaceuticals can directly inhibit the growth of gut bacteria, but degree to which such interactions manifest in complex community settings is an open question. Here, we compared effects 30 drugs on a 32-species synthetic with their each member isolation. While most individual drug-species remained same context, communal behaviors emerged 26% all tested cases. Cross-protection during drug-sensitive species were protected was 6 times more frequent than cross-sensitization, converse...
Quantitative analysis of microbial growth curves is essential for understanding how bacterial populations respond to environmental cues. Traditional approaches make parametric assumptions about the functional form these curves, limiting their usefulness studying conditions that distort standard curves. In addition, modern robotics platforms enable high-throughput collection large volumes data, thus requiring strategies can analyze large-scale data in a flexible and efficient manner. Here, we...
Summary Pharmaceuticals can directly inhibit the growth of gut bacteria, but degree such interactions manifest in complex community settings is an open question. Here we compared effects 30 drugs on a 32-species synthetic with their each member isolation. While most individual drug–species remained same context, communal behaviors emerged 26% all tested cases. Cross-protection, during which drug-sensitive species became protected community, was 6-times more frequent than cross-sensitization,...
Genome-scale metabolic networks provide a comprehensive structural framework for modeling genotype-phenotype relationships through flux simulations. The solution space the state of cell is typically very large and optimization-based approaches are often necessary predicting active under specific environmental conditions. objective function to be used in such optimization algorithms directly linked with biological hypothesis underlying model therefore it one most relevant parameters...
Summary Toxic bacterial modules, in particular toxin-antitoxin (TA) systems, have been long sought-after for their antimicrobial potential, although with limited success 1–6 . Here we show that the cyclic-oligonucleotide-based antiphage signaling system (CBASS), another example of a toxic module, increases sensitivity to well-established antifolate antibiotics, interferes synergy, and ultimately enables lysis by antifolates - classic bacteriostatic Vibrio cholerae We propose molecular...
Synthetic communities can help uncover metabolic forces shaping microbial ecosystems. Yet, in case of the gut microbiota, culturing undefined media has prevented detection dependencies. Here we show, using chemically defined media, how species survival is jointly determined by supplied resources and community metabolism. We used 63 representative bacterial strains varied inoculum compositions to assemble stable 14 media. Over 95% showed markedly improved or diminished performance relative...
Abstract The rise in antimicrobial resistance poses a worldwide threat, reducing the efficacy of common antibiotics. Determining activity new chemical compounds through experimental methods is still time-consuming and costly endeavor. Compound-centric deep learning models hold promise to speed up this search prioritization process. Here, we introduce lightweight computational strategy for discovery that builds on MolE ( Mol ecular representation redundancy reduced E mbedding), framework...
Abstract Transport of small molecules across the bacterial cell envelope is essential to ensure nutrient uptake and protect bacteria from toxic compounds. Due its decisive role, transport controlled by complex regulatory networks, knowledge which – in particular external cues remains poorly understood. Here we investigate transcriptional regulation seven prominent genes Escherichia coli 94 defined chemical cues, simultaneously map contributions key regulators MarA, SoxS Rob promoter...
Abstract Drug combinations present a powerful strategy to tackle antimicrobial resistance, but have not been systematically tested in many bacterial species. Here, we used an automated high-throughput setup profile ∼ 8000 between 65 antibacterial drugs three Gram-positive species: the model species, Bacillus subtilis and two prominent pathogens, Staphylococcus aureus Streptococcus pneumoniae . Thereby, recapitulate previously known drug interactions, also identify ten times more interactions...
Introduction: Although endocarditis caused by Borrelia burgdorferi is rare, this case report relevant because it stimulates reflection on the core competencies of family physician. Case report: A 36 year-old male patient with a history arterial hypertension and cardiac surgery in adolescence for repair an interventricular communication, presented four-month vertigo, hemianopsia, headache, memory loss, hemiparesis, facial paralysis dysarthria. Tomography brain showed ischemic lesions. The...