A5091798048- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Galectins and Cancer Biology
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- CRISPR and Genetic Engineering
- Ubiquitin and proteasome pathways
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Immune Response and Inflammation
- Immune cells in cancer
Broad Institute
2023-2024
The Ohio State University
2020-2024
Children's Hospital of Philadelphia
2024
A hallmark of high-risk childhood medulloblastoma is the dysregulation RNA translation. Currently, it unknown whether dysregulates translation putatively oncogenic non-canonical open reading frames (ORFs). To address this question, we performed ribosome profiling 32 tissues and cell lines observed widespread ORF We then developed a stepwise approach using multiple CRISPR-Cas9 screens to elucidate ORFs putative microproteins implicated in survival. determined that lncRNA-ORFs upstream (uORFs)...
Introduction Therapeutic antibodies have become a major strategy to treat oncologic diseases. For chronic lymphocytic leukemia, against CD20 are used target and elicit cytotoxic responses malignant B cells. However, efficacy is often compromised due suppressive microenvironment that interferes with cellular immune responses. To overcome this suppression, agonists of pattern recognition receptors been studied which promote direct cytotoxicity or anti-tumoral NOD2 an intracellular receptor...
Abstract TLRs, a family of membrane-bound pattern recognition receptors found on innate immune cells, have been well studied in the context cancer therapy. Activation these has shown to induce inflammatory anticancer events, including differentiation and apoptosis, across wide variety malignancies. In contrast, intracellular such as NOD-like minimally studied. NOD2 is member receptor that initiates signaling response bacterial motif muramyl dipeptide. this study, we examined influence human...
Summary A hallmark of high-risk childhood medulloblastoma is the dysregulation RNA translation. Currently, it unknown whether dysregulates translation putatively oncogenic non-canonical open reading frames. To address this question, we performed ribosome profiling 32 tissues and cell lines observed widespread ORF We then developed a step-wise approach to employ multiple CRISPR-Cas9 screens elucidate functional ORFs implicated in survival. determined that lncRNA-ORFs upstream frames (uORFs)...
Abstract BACKGROUND Cell surface proteins are valuable for generation of targeted and immune-based cancer therapies. While large molecular datasets available multiple central nervous system (CNS) tumors, identifying such targets on a tumor-level scale to generate novel therapeutics patients is limited by lack analytical pipelines. Here, we present CARTarT (CAR-T Target identification Tool), no-code, web application that utilizes user-inputted bulk transcriptomic data identify putative...
Abstract High-risk medulloblastoma is one of the most recalcitrant pediatric cancers, and children with MYC-amplified disease frequently succumb to relapsed disease. Extensive analyses coding genome in this have characterized additional somatic events some subsets patients, though tumors lack targetable mutations do not yield insights regarding their aggressive behavior. At same time, known exhibit extensive rewiring translational control MYC-driven tumors, consistent recent genetic evidence...