A5032947645- Skin and Cellular Biology Research
- CRISPR and Genetic Engineering
- Hydrogen embrittlement and corrosion behaviors in metals
- Cellular Mechanics and Interactions
- Material Properties and Failure Mechanisms
- Cell Adhesion Molecules Research
- Animal Genetics and Reproduction
- Transgenic Plants and Applications
- Autoimmune Bullous Skin Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Dermatological and Skeletal Disorders
- Hair Growth and Disorders
- Engineering Diagnostics and Reliability
- Click Chemistry and Applications
- Viral Infections and Immunology Research
- melanin and skin pigmentation
- Soil and Environmental Studies
- Botanical Research and Applications
- Telomeres, Telomerase, and Senescence
- Agricultural Productivity and Crop Improvement
- Skin Protection and Aging
- Wound Healing and Treatments
- Cholinesterase and Neurodegenerative Diseases
- Postharvest Quality and Shelf Life Management
- Neurological Disease Mechanisms and Treatments
Pirogov Russian National Research Medical University
2020-2024
Institute of Higher Nervous Activity and Neurophysiology
2021
Lomonosov Moscow State University
2019
Central Research and Design Institute
1980-1989
Fruit and berry raw materials are a valuable source of nutrients in the diet population Sverdlovsk Region. Among crops, blue honeysuckle (Lonicera caerulea L.) attracts special attention due to its rich biochemical composition, ease cultivation wide distribution Urals. The purpose study is determine organoleptic physicochemical (mass fraction reducing total sugars, acidity) indicators from fruits. Generally accepted standard research methods were used. material for was fruits varieties...
Propriospinal neurons relay sensory and motor information across the spinal cord are critical components of circuits coordinating body movements. Their diversity roles in control not clearly defined yet. In this study, by combining anatomical, molecular, functional analyses mice, we identified characterized an ascending subtype propriospinal belonging to Pitx2+ V0 family neurons. We found that integrated sensorimotor their function is important for execution precise limb movements required...
The recessive form of dystrophic epidermolysis bullosa (RDEB) is a crippling disease caused by impairments in the junctions dermis and basement membrane epidermis. Using ectopic expression hTERT/hTERT + BMI-1 primary cells, we developed expansible cultures RDEB fibroblasts keratinocytes. We showed that they display properties their founders, including morphology, contraction ability respective specific markers reduced secretion type VII collagen (C7). immortalized keratinocytes retained...
The recessive form of dystrophic epidermolysis bullosa (RDEB) is a debilitating disease caused by impairments in the junctions dermis and basement membrane epidermis. Mutations COL7A1 gene induce multiple abnormalities, including chronic inflammation profibrotic changes skin. However, correlations between specific mutations their phenotypic output remain largely unexplored. gene, described here, were found DEB register. Among them, two homozygous cases compound heterozygous identified. We...
Abstract A new stable line of human keratinocytes was obtained. The cells have altered morphology, both abnormal chromosomal composition and expression keratinocyte markers, do not show contact inhibition, could be cultured in various media limited stratification ability vitro. Upon transplantation into nude mice the tumorigenic properties.
Background: The development of cholinergic deficit is considered an early sign a number pathological conditions, including Alzheimer’s disease. Cholinergic dysfunction underlies cognitive decline associated with both normal aging and Objective: Here, we studied possible mechanism functional impairment neurons using olfactory bulbectomy model. Methods: Male mice were subjected to or sham surgery. Three weeks after that they trained in Morris water maze then euthanized one month indices as...
This study aimed to enhance homology-directed repair (HDR) efficiency in CRISPR/Cas-mediated genome editing by targeting three key factors regulating the balance between HDR and non-homologous end joining (NHEJ): MAD2L2, SCAI, Ligase IV. In order achieve this, a cellular model using mutated eGFP was designed monitor events. Results showed that MAD2L2 knockdown SCR7 treatment significantly improved during Cas9-mediated of gene HEK293T cell line. Fusion protein Cas9-SCAI did not improve HDR....
Here we described two unrelated cases of a new pathogenic variant in COL7A1, the deletion c.2588-3_2588delCAGC. The causes alternative splicing preceding exon and results truncation fibronectin III (FN-III) like domain C7. This is distinct from previously documented variants as it does not completely exclude 19, restores open reading frame COL7A1 less severe form RDEB intermediate.
Epidermolysis bullosa simplex (EBS) is a dermatological condition marked by skin fragility and blister formation resulting from separation within the basal layer of epidermis, which can be attributed to various genetic etiologies. This study presents three pathogenic de novo variants in young children, with clinical manifestations appearing as early neonatal period. The contribute EBS phenotype through two distinct mechanisms: direct keratin abnormalities due Krt14 gene, indirect effects via...
Abstract Recessive Dystrophic Epidermolysis Bullosa (RDEB) is a debilitating genodermatosis caused by pathogenic mutations in the COL7A1 gene, which induce absence or reduction number of anchoring fibrils. The severity RDEB depends on mutation type and localization, but many aspects this dependence remain to be elucidated. Here, we report novel variant Intermediate two unrelated patients. Their disease manifestation includes early skin oral mucosa blistering associated with localized...
Жимолость синяя (Lonicera caerulea L., Caprifoliaceae) – перспективная ягодная культура. В настоящее время находится на этапе широкого внедрения в промышленное производство, которое предъявляет особые требования к новым сортам. Потенциальные возможности культуры далеко не исчерпаны, что вызывает большой интерес жимолости у селекционеров как Российской Федерации, так и за рубежом. Объектом наших исследований служили сеянцы трех семей жимолости, полученные из семян от свободного опыления...