A5039233729- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Retinal Development and Disorders
- Retinal Imaging and Analysis
- Photosynthetic Processes and Mechanisms
- Cancer Cells and Metastasis
- Inhalation and Respiratory Drug Delivery
- Retinal Diseases and Treatments
- Genomics and Phylogenetic Studies
- Neuroscience and Neural Engineering
- Nerve injury and regeneration
- Engineering Applied Research
- Photopolymerization techniques and applications
- Single-cell and spatial transcriptomics
- Plant Virus Research Studies
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Plant Molecular Biology Research
- Microfluidic and Bio-sensing Technologies
- Tissue Engineering and Regenerative Medicine
- Respiratory viral infections research
- Cell Image Analysis Techniques
- Respiratory Support and Mechanisms
- Additive Manufacturing and 3D Printing Technologies
- Epoxy Resin Curing Processes
National Center for Advancing Translational Sciences
2020-2025
National Institutes of Health
2015-2024
Shanghai Ocean University
2024
Hybrid Rapeseed Research Center of Shaanxi Province
2022-2023
Northwest A&F University
2022-2023
National Eye Institute
2015-2022
Nanjing Forestry University
2020-2021
Case Western Reserve University
2010-2020
University of Pennsylvania
2015-2019
Office of Extramural Research
2019
This article describes a method for the biofabrication of skin tissue equivalents in multiwell plate format. The technique and results overcome shortcomings previously published engineering methods, show good architecture barrier function from well to well; thus it may be used compound functional testing development disease models screening.
Three-dimensional (3D) biofabrication techniques enable the production of multicellular tissue models as assay platforms for drug screening. The increased cellular and physiological complexity in these 3D should recapitulate relevant biological environment found body. Here we describe use bioprinting to fabricate skin equivalent tissues varying complexity, including human epidermis, non-vascularized vascularized full-thickness equivalents, a multi-well platform Human keratinocytes,...
Abstract A 3D bioprinted neurovascular unit (NVU) model is developed to study glioblastoma (GBM) tumor growth in a brain‐like microenvironment. The NVU includes human primary astrocytes, pericytes and brain microvascular endothelial cells, patient‐derived cells (JHH‐520) are used for this study. Fluorescence reporters with confocal high content imaging quantitate real‐time network formation growth. Extensive validation of the NVU‐GBM immunostaining relevant cellular markers extracellular...
The COVID-19 pandemic has highlighted the need for human respiratory tract-based assay platforms efficient discovery and development of antivirals disease-modulating therapeutics. Physiologically relevant tissue models lower tract (LRT), including bronchioles alveolar sacs, are high interest because they primary site severe SARS-CoV-2 infection most affected during terminal stage COVID-19. Current epithelial lung used to study viral infections include cells at air-liquid interface (ALI) with...
Abstract There is a critical need for physiologically relevant, robust, and ready-to-use in vitro cellular assay platforms to rapidly model the infectivity of emerging viruses develop new antiviral treatments. Here we describe complexity human alveolar tracheobronchial air liquid interface (ALI) tissue models during SARS-CoV-2 influenza A virus (IAV) infections. Our results showed that both IAV effectively infect these ALI tissues, with exhibiting slower replication peaking at later...
During mesenchymal condensation, the initial step of skeletogenesis, transduction minute mechanical forces to nucleus is associated with up or down-regulation genes, ultimately resulting in formation skeletal template and appropriate cell lineage commitment. The summation these biophysical cues affects cell's shape fate. Here, we predict measure surface strain, live stem cells, response controlled delivery stresses, providing a platform direct short-term structure - function relationships...
Assessing skin irritation potential is critical for the safety evaluation of topical drugs and other consumer products such as cosmetics. The use advanced cellular models, an alternative to replace animal testing in both ingredients, already mandated by law European Union (EU) countries. However, there has not yet been a large-scale comparison effects topical-use compounds different models. This study assesses models that are compatible with high throughput screening (HTS) platforms. A set...
The leucine-rich repeat receptor-like protein kinase (LRR-RLK) family represents the largest group of RLKs in plants and plays vital roles plant growth, development responses to environmental stress. Although LRR-RLK families have been identified many species, they not yet reported B. napus. In this study, a total 444 BnLRR-RLK genes were genome Brassica napus cultivar "Zhongshuang 11" (ZS11), classified into 22 subfamilies based on phylogenetic relationships genome-wide analyses. Conserved...
Abstract The tumor microenvironment (TME) is the cellular environment in which cancer cell exist and made up of a combination stroma cells extra matrix (ECM), secreted biomolecules, including cytokines, from both cells. TME composition drives growth drug resistance. We previously established 96-wells based bioprinted glioblastoma (GBM) model that includes neurovascular unit (NVU) endothelial cells, astrocytes pericytes. demonstrated its applicability for screening by using high content...
Abstract 3D tissue models that mimic the physiology of human tissues and organs are being developed in multi-well plate format as predictive assays for drug screening. bioprinting enables fabrication with precision reproducibility a 96-well medium throughput We have established robust protocols vascularized lung using primary pulmonary endothelial cells constitutively expressing green fluorescent protein (GFP), pericytes, fibroblasts mixed fibrin-based hydrogel. create model ring-shaped...
Stellera chamaejasme Linn, an important poisonous plant of the China grassland, is toxic to humans and livestock. The rapid expansion S. has greatly damaged grassland ecology and, consequently, seriously endangered development animal husbandry. To draft efficient prevention control measures, it become more urgent carry out research on its adaptive mechanisms in different unfavorable habitats at genetic level. Quantitative real-time polymerase chain reaction (qRT-PCR) a widely used technique...
Future climate change will have serious impacts on species survival and distribution likely lead to the extinction of some classified as endangered. Carpinus tientaiensis (Betulaceae), a unique endangered in China, has restricted small population, indicating an urgent need for its protection. However, research current or influence that future is limited. We used MaxEnt model ArcGIS software predict niches C. tientaiensis. The suitable area small, mainly east south Zhejiang Anhui, central...
A major hurdle to understanding and exploiting interactions between the stem cell its environment is lack of a tool for precise delivery mechanical cues concomitant observing sub-cellular adaptation structure. These studies demonstrate use microscale particle image velocimetry (μ-PIV) in situ spatiotemporal mapping flow fields around mesenchymal cells, i.e. murine embryonic multipotent line C3H10T1/2, at subcellular length scale, providing real time observation analysis prevailing milieu. In...
A wide range of complex in vitro models (CIVMs) are being developed for scientific research and preclinical drug efficacy safety testing. The hope is that these CIVMs will mimic human physiology pathology predict clinical responses more accurately than the current cellular models. integration into discovery development pipeline requires rigorous validation, including cellular, morphological, functional characterization; benchmarking biomarkers; operationalization as robust reproducible...
Cytoplasmic male sterility (CMS) is the main mechanism employed to utilize heterosis of Brassica napus. CMS three-line rapeseed hybrids have dramatically enhanced yield and brought about global revolution hybrid varieties, replacing conventional crop varieties. Over last half century, China has led development napus Two sterile lines, polima (pol) shaan 2A, were particular importance for establishment systems in rapeseed, which opened up a new era utilization. However, current breeding...
Abstract Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is the third coronavirus in less than 20 years to spillover from an animal reservoir and cause severe disease humans. High impact viruses such as pathogenic beta-coronaviruses influenza viruses, well other emerging pose ongoing global health threat There a critical need for physiologically relevant, robust ready use, vitro cellular assay platforms rapidly model infectivity of discover develop new antiviral treatments....