Polymerisation of haematin to β‐haematin (haemozoin or malaria pigment) in acidic acetate solutions was studied using infrared spectroscopy. The reaction found occur spontaneously between 6 and 65°C, 0.1–4.5 M pH 4.2–5.0. anti‐malarial drugs quinine, chloroquine amodiaquin were block spontaneous formation, while the anti‐malarially inactive 9‐epiquinine 8‐hydroxyquinoline had no effect on reaction, as did primaquine, a drug which is active only against exo‐erythrocytic stages infection. It...

Local heat transfer data were obtained for turbulent natural convection on vertical and inclined upward downward facing surfaces. The test surface consisted of a 1.83 m (6 ft) wide × 7.32 (24 high plate with constant flux by electrical resistive heating metal foil the surface. tests conducted in air modified Grashof numbers up to 1015. Measurements made local temperature this condition, at angles from 30 deg (upward facing, unstable) through 80 (downward stable). results show location...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMechanism of iron release from human serum C-terminal monoferric transferrin to pyrophosphate: kinetic discrimination between alternative mechanismsTimothy J. Egan, David C. Ross, Langley R. Purves, and Paul A. AdamsCite this: Inorg. Chem. 1992, 31, 11, 1994–1998Publication Date (Print):May 1, 1992Publication History Published online1 May 2002Published inissue 1 1992https://doi.org/10.1021/ic00037a005RIGHTS & PERMISSIONSArticle...

The effect of increasing concentrations the nonionic detergent Triton X-100 on catalytic activity, stability, phospholipid content, and aggregational state solubilized Ca2+ ion activated adenosinetriphosphatase (Ca2+-ATPase) sarcoplasmic reticulum has been investigated. Increasing in range 0.2-0.6% (w/v) inhibited ATP hydrolysis p-nitrophenyl phosphate parallel to extent 50% 95%, respectively. Inactivation by preincubation excess ethylene glycol bis(beta-aminoethyl...

Spontaneous formation of beta-haematin (malaria pigment) from haematin in acetate solution follows pseudo-zero-order and not autocatalytic kinetics. Acetate appears to facilitate the reaction by solubilizing acting as a phase-transfer catalyst, role which, vivo, could be fulfilled carboxylic acids or amino acids.

Abstract Limited reaction of glutaraldehyde with the Ca2+-ATPase (Mr approximately 110,000) sarcoplasmic reticulum results in intramolecular cross-linking at active site, which can be detected by an anomalous increase apparent molecular weight 125,000) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Ross D.C., and McIntosh D.B. (1987) J. Biol. Chem. 262, 2042-2049). ATP, ADP, AMPPCP, trinitrophenyladenosine triphosphate, decavanadate inhibited cross-link a manner suggestive...

Glutaraldehyde treatment of sarcoplasmic reticulum vesicles results in formation cross-linked Ca2+-ATPase oligomers. Under limiting reaction conditions, where minimal interpolypeptide cross-linking occurs, hydrodynamic properties the monomer are altered, such that, on sodium dodecyl sulfate-polyacrylamide electrophoresis, enzyme migrates with an apparent molecular weight 125,000 (E(125], as compared to native (E(110]. The E(125) species was also formed following other bis-aldehydes,...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInhibition of larval growth in Spodoptera frugiperda by sublethal dietary concentrations insecticidesDavid C. Ross and Thomas M. BrownCite this: J. Agric. Food Chem. 1982, 30, 1, 193–196Publication Date (Print):January 1982Publication History Published online1 May 2002Published inissue 1 January 1982https://pubs.acs.org/doi/10.1021/jf00109a045https://doi.org/10.1021/jf00109a045research-articleACS PublicationsRequest reuse permissionsArticle...

The effects of thermal insult on living tissue have been studied by direct microscopic observation the circulatory system’s response to a controlled trauma regimen. An experimental apparatus has developed which utilizes unique high and low temperature stage in conjunction with precision control system examine injury process microcirculation golden hamster cheek pouch. Unique features this are: (1) continuous monitoring processes at cellular level, (2) capability for quantitative assay...

Intramolecular crosslinking of the active site sarcoplasmic reticulum Ca(2+)-ATPase with glutaraldehyde results in substantial inhibition ATPase activity and stabilization ADP-sensitive E1 approximately P(2Ca) intermediate (E, enzyme) occluded Ca2+ [Ross, D. C., Davidson, G. A. & McIntosh, B. (1991) J. Biol. Chem. 266, 4613-4621]. We show here, using conditions low passive vesicle permeability absence ADP, that "deoccludes" more rapidly than it leaks out lumen. Deocclusion is paralleled by...

Epidermal wound healing is a complex process that repairs injured tissue. The complexity of this increases when bacteria are present in wound; the interaction determines whether infection sets in. Because underlying physiological problems infected wounds do not follow normal pattern. In paper we mathematical model both and uninfected wounds. At core our an account initiation angiogenesis by macrophage-derived growth factors. We express as system reaction-diffusion equations, results...

Investigation of the properties Ca2(+)-ATPase sarcoplasmic reticulum cross-linked at active site with glutaraldehyde showed that ATP binding affinity and rate ATP-dependent phosphorylation Ca2+ occlusion were decreased 2-3 orders magnitude compared native enzyme. Cross-linkage had little effect on or marginally increased acetyl phosphate- p-nitrophenyl phosphate-supported occlusion. Ca2(+)-induced changes in tryptophan fluorescence unaffected. High levels phosphoenzyme (up to 4 nmol/mg...

Purpose This paper aims to numerically investigate the nanofluid flow, heat transfer and entropy generation during natural convection in an annulus. Design/methodology/approach The lattice Boltzmann method is used simulate velocity temperature fields. Furthermore, some special modifications are applied make capable for simulation curved boundary conditions. annulus filled with CuO-water nanofluid. dynamic viscosity of estimated using KLL (Koo-Kleinstreuer-Li) model, nanoparticle shape effect...

Monomeric Ca2+-ATPase of skeletal muscle sarcoplasmic reticulum dispersed in Triton X-100 is stoichiometrically phosphorylated from Pi a Ca2+-depleted medium containing dimethyl sulfoxide and catalyzes efficient (80%) phosphoryl transfer to ADP following jump water activity the presence Ca2+. The Ca2+ concentration dependence ATP synthesis was sigmoidal (nH = 1.7) millimolar range (K0.5 0.3 mM), indicating involvement at least two low affinity binding sites. These results, taken together...

Purpose The purpose of this paper is to investigate the compact finite-difference lattice Boltzmann method used simulate free convection within a cavity. Design/methodology/approach discretization enables numerical simulations be run when there are non-uniform and curvilinear grids with finer near-wall grid resolution. Furthermore, high-order applied in approach, which makes it possible go relatively coarse mesh respect simulations, classical method. configuration cavity set sine-walled...