A5029867256- Ion Channels and Receptors
- Neurobiology and Insect Physiology Research
- Biochemical Analysis and Sensing Techniques
- Magnesium in Health and Disease
- Ion channel regulation and function
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- biodegradable polymer synthesis and properties
- Lipid Membrane Structure and Behavior
- Mitochondrial Function and Pathology
- Nanopore and Nanochannel Transport Studies
- Neuroendocrine regulation and behavior
- Postharvest Quality and Shelf Life Management
- Menstrual Health and Disorders
- Metabolism and Genetic Disorders
- Plant Stress Responses and Tolerance
- Pain Mechanisms and Treatments
- Bacterial Genetics and Biotechnology
- Biochemical Acid Research Studies
- Phytochemicals and Antioxidant Activities
- RNA and protein synthesis mechanisms
- Biochemical effects in animals
- ATP Synthase and ATPases Research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Circadian rhythm and melatonin
- Streptococcal Infections and Treatments
University of Illinois Urbana-Champaign
2013-2021
Illinois College
2013-2021
University of Illinois Chicago
2013-2020
Peoria campus of the University of Illinois System
2014-2018
Illinois CancerCare
2015
Rutgers New Jersey Medical School
2009-2013
Rutgers, The State University of New Jersey
2009-2013
University Hospital, Newark
2012
Michigan State University
2003-2006
Yerevan State University
1996
The transient receptor potential melastatin 8 (TRPM8) ion channel is a major sensor of environmental cold temperatures. It activated by and chemical agonists, such as menthol icilin. activation these channels both cooling agents requires the presence membrane phospholipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P 2 ]. mechanism TRPM8 physical factors unknown, involvement cellular signaling pathways has been considered. Here we have characterized gating rat reconstituted in planar lipid...
Polyphosphate (polyP) is an inorganic polymer built of tens to hundreds phosphates, linked by high-energy phosphoanhydride bonds. PolyP forms complexes and modulates activities many proteins including ion channels. Here we investigated the role polyP in function transient receptor potential melastatin 8 (TRPM8) channel. Using whole-cell patch-clamp fluorescent calcium measurements demonstrate that enzymatic breakdown exopolyphosphatase (scPPX1) inhibits channel activity human embryonic...
Testosterone is a key steroid hormone in the development of male reproductive tissues and regulation central nervous system. The rapid signaling mechanism induced by testosterone affects numerous behavioral traits, including sexual drive, aggressiveness, fear conditioning. However, currently identified receptor(s) not believed to underlie fast signaling, suggesting an orphan pathway. Here we report that ion channel from transient receptor potential family, TRPM8, commonly known as cold...
Oxytocin is a hormone with various actions. Oxytocin-containing parvocellular neurons project to the brainstem and spinal cord. release from these suppresses nociception of inflammatory pain, molecular mechanism which remains unclear. Here, we report that noxious stimulus receptor TRPV1 an ionotropic oxytocin receptor. elicits activity in native heterologous expression systems, regardless presence classical In knockout mice, DRG exhibit reduced sensitivity relative controls, injections...
Opioids, agonists of µ-opioid receptors (µORs), are the strongest pain killers clinically available. Their action includes a strong central component, which also causes important adverse effects. However, µORs found on peripheral endings nociceptors and their activation there produces meaningful analgesia. The cellular mechanisms downstream not well understood. Here, we show in neurons murine dorsal root ganglia that pro-nociceptive TRPM3 channels, present parts nociceptors, strongly...
Zn 2+ , Mg and Ca are essential minerals required for a plethora of metabolic processes signaling pathways. Different categories cation-selective channels transporters therefore to tightly control the cellular levels individual metals in cell-specific manner. However, mechanisms responsible organismal balance these poorly understood. Herein, we identify central indispensable role channel-kinase TRPM7 mineral homeostasis. The function was assessed by single-channel analysis TRPM7, phenotyping...
Significance The increase in obesity has been accompanied by a rise the prevalence of peripheral neuropathy. Because there is no disease-modifying therapy, paradigm-shifting research on pain associated with obesity, metabolic syndrome, and type 2 diabetes urgently needed. Our data suggest that fecal transplantation from lean to obese, insulin-resistant mice decreases obesity-induced hypersensitivity increases nerve density skin. These improvements are changes system gene expression...
Abstract Mitochondrial permeability transition pore (mPTP) opening allows free movement of ions and small molecules leading to mitochondrial membrane depolarization ATP depletion that triggers cell death. A multi-protein complex the synthase has an essential role in mPTP. However, molecular identity central 'pore' part mPTP is not known. highly purified fraction mammalian mitochondria containing C-subunit ATPase (C-subunit), calcium, inorganic polyphosphate (polyP) polyhydroxybutyrate (PHB)...
// Swapna Asuthkar 1 , Kiran Kumar Velpula Pia A. Elustondo 2 Lusine Demirkhanyan and Eleonora Zakharian University of Illinois College Medicine, Department Cancer Biology Pharmacology, Peoria, IL, USA Dalhousie University, Halifax, NS, Canada Correspondence to: Zakharian, email: Asuthkar, Keywords : prostate cancer (PC), transient receptor potential melastatin 8(TRPM8) ion channel, testosterone, DHT (5α-dihydrotestosterone), androgen (AR) Received March 18, 2015 Accepted April 07, Published...
The regulation of the heat- and capsaicin-activated transient receptor potential vanilloid 1 (TRPV1) channels by phosphoinositides is controversial. Data in cellular systems support dependence TRPV1 activity on phosphoinositides. purified TRPV1, however, was recently shown to be fully functional artificial liposomes absence Here, we show that several other negatively charged phospholipids, including phosphatidylglycerol, can also excised patches at high concentrations. When incorporated into...
The TRPM8 ion channel is expressed in sensory neurons and responsible for sensing environmental cues, such as cold temperatures chemical compounds, including menthol icilin. functional activity regulated by various physical factors likely to be preconditioned its molecular composition. Our studies indicate that the forms a structural-functional complex with polyester poly-(R)-3-hydroxybutyrate (PHB). We identified mass spectrometry number of PHB-modified peptides N terminus protein...
TRPM8 is a polymodal, nonselective cation channel activated by cold temperature and cooling agents that plays critical role in the detection of environmental cold. We found pharmacological target tacrolimus (FK506), macrolide immunosuppressant with several clinical uses, including treatment organ rejection following transplants, atopic dermatitis, dry eye disease. Tacrolimus an inhibitor phosphatase calcineurin, action shared cyclosporine. activates channels different species, humans,...
Transient receptor potential vanilloid 6 (TRPV6) channels play an important role in Ca2+ absorption the intestines. Both phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] and cytoplasmic ATP have been proposed to be for maintaining TRPV6 activity. To evaluate whether PtdIns(4,5)P2 affect channel activity directly or indirectly, we used a dual approach, examining excised patches planar lipid bilayers. In inside-out patch-clamp measurements, reactivated human after current rundown only...
ABSTRACT Kingella kingae is a human oral bacterium that can cause diseases of the skeletal system in children and infective endocarditis adults. K. produces toxin RTX group, RtxA. To investigate role RtxA disease pathogenesis vivo , strain PYKK081 its isogenic RtxA-deficient KKNB100 were tested for their virulence pathological consequences upon intraperitoneal injections 7-day-postnatal (PN 7) rats. At doses above 8.0 × 10 6 cells/animal, was able to fatal illness, resulting rapid weight...
The transient receptor potential melastatin (TRPM)-3 channel is critical for various physiologic processes. In somatosensory neurons, TRPM3 has been implicated in temperature perception and inflammatory hyperalgesia, whereas pancreatic β-cells the linked to glucose-induced insulin release. As a typical representative of TRP family, highly polymodal. cells, it activated by heat chemical agonists, including pregnenolone sulfate (PS) nifedipine (Nif). To define nuances activity its modulators,...