A5085362664- Mass Spectrometry Techniques and Applications
- Tardigrade Biology and Ecology
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Advanced Proteomics Techniques and Applications
- Peptidase Inhibition and Analysis
- Doctoral Education Challenges and Solutions
- Hemoglobin structure and function
- Higher Education Research Studies
- Career Development and Diversity
- RNA modifications and cancer
- Physiological and biochemical adaptations
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
Johns Hopkins University
2022-2024
Interface (United States)
2022-2023
Oxidative stress induces a wide range of cellular damage, often causing disease and cell death. While many organisms are susceptible to the effects oxidative stress, haloarchaea have adapted be highly resistant. Several aspects haloarchaeal response been characterized, however little is known about impacts at translation level. Using model archaeon Haloferax volcanii , we performed RNA-seq ribosome profiling (Ribo-seq) characterize global landscape during stress. We identified 281 genes with...
One of the planet's more understudied ecosystems is deep biosphere, where organisms can experience high hydrostatic pressures (30–110 MPa); yet, by current estimates, these subsurface and ocean zones host majority Earth's microbial animal life. The extent to which terrestrially relevant up 100 MPa deform most globular proteins—and kinds—has not been established. Here, we report invention an experimental apparatus that enables structural proteomic methods be carried out at for first time....
Abstract Cellular desiccation - the loss of nearly all water from cell is a recurring stress in an increasing number ecosystems that can drive protein unfolding and aggregation. For cells to survive, at least some proteome must resume function upon rehydration. Which proteins tolerate desiccation, molecular determinants underlie this tolerance, are largely unknown. Here, we apply quantitative structural proteomic mass spectrometry show certain possess innate capacity rehydration following...