A5065314600- HIV Research and Treatment
- Immune Cell Function and Interaction
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Monoclonal and Polyclonal Antibodies Research
- Mental Health Treatment and Access
- Adolescent Sexual and Reproductive Health
- Child and Adolescent Psychosocial and Emotional Development
- Advanced biosensing and bioanalysis techniques
- Health, psychology, and well-being
- Cytomegalovirus and herpesvirus research
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Hepatitis C virus research
- Chronic Lymphocytic Leukemia Research
- vaccines and immunoinformatics approaches
- Natural product bioactivities and synthesis
- Essential Oils and Antimicrobial Activity
Sefako Makgatho Health Sciences University
2023
National Research Foundation
2022
National Health Laboratory Service
2011-2014
National Institute for Communicable Diseases
2009-2014
University of Limpopo
2009
An understanding of how broadly neutralizing activity develops in HIV-1-infected individuals is needed to guide vaccine design and immunization strategies. Here we used a large panel 44 HIV-1 envelope variants (subtypes A, B, C) evaluate the presence antibodies serum samples obtained 3 years after seroconversion from 40 women enrolled CAPRISA 002 acute infection cohort. Seven participants had that neutralized more than 40% viruses tested were considered have neutralization breadth. Among...
ABSTRACT The targets of broadly cross-neutralizing (BCN) antibodies are great interest in the HIV vaccine field. We have identified a subtype C HIV-1-superinfected individual, CAP256, with high-level BCN activity, and characterized antibody specificity mediating breadth. CAP256 developed potent activity peaking at 3 years postinfection, neutralizing 32 (76%) 42 heterologous viruses, titers against some viruses exceeding 1:10,000. showed bias, preferentially A over B viruses. serum targeted...
ABSTRACT We identified three cross-neutralizing plasma samples with high-titer anti-membrane proximal external region (MPER) peptide binding antibodies from among 156 chronically human immunodeficiency virus type 1-infected individuals. In order to establish if these were directly responsible for the observed neutralization breadth, we used MPER-coated magnetic beads deplete plasmas of specific antibodies. Depletion anti-MPER BB34, CAP206, and SAC21 resulted in 77%, 68%, 46% decreases,...
Entry of human immunodeficiency virus type 1 (HIV-1) into cells is mediated by the virion surface envelope (Env) glycoproteins, making it a desirable target for antiretroviral entry inhibitors. We previously isolated family gp120 binding RNA aptamers and showed that they neutralized infectivity HIV-1. In this study, we assessed activity shortened synthetic derivative B40 aptamer, called UCLA1, against large panel HIV-1 subtype C viruses. UCLA1 tightly bound to consensus isolates same with...
Substance abuse brings major negative social and health impacts in South Africa. Nyaope, a cocktail drug commonly used the Tshwane townships, has been well documented to be highly addictive very difficult quit. The resultant difficulties include financial, social, mental, specifically depression anxiety. This study aims quantify levels among family members with nyaope users Tshwane, quantitative cross-sectional design collect data nine communities. patient questionnaire-9 (PHQ-9) screening...
Identification of the epitopes targeted by antibodies that can neutralize diverse HIV-1 strains provide important clues for design a preventative vaccine.We have developed computational approach identify key amino acids within envelope glycoprotein influence sensitivity to broadly cross-neutralizing antibodies. Given sequence alignment and neutralization titers panel viruses, method works fitting phylogenetic model allows acid frequencies at each site depend on sensitivities. Sites which...
To investigate whether distinct populations have differing human immunodeficiency virus type 1 (HIV) neutralizing antibody responses, we compared 20 women from Tanzania's HIV Superinfection Study (HISIS) cohort, who were infected multiple subtypes, and 22 the Centre for AIDS Programme of Research in South Africa (CAPRISA) exclusively with subtype C. By 2 years after infection, 35% HISIS subjects developed neutralization breadth, 9% CAPRISA (P = .0131). Cumulative viral loads between 3 12...
The leaves of Dicerocaryum senecioides are used in South Africa as a traditional remedy for many ailments, including inflammatory disorders. purpose this study was therefore to evaluate the anti-inflammatory potential dichloromethane extract D. leaves. Methanol extracts were sub-fractionated with and fraction investigated according its effects on mitogen-induced proliferative responses cytokine profiles isolated human blood mononuclear leucocytes (MNL). cells pre-treated (25-100 microg...
Background: Nyaope is a strongly addictive novel psychoactive substance that commonly used in predominantly black townships South Africa. The undesired behaviours of the users result family members developing mental health challenges. often commit petty crimes, including stealing from families and neighbours.Aim: aim this study was to quantify anxiety symptoms among nyaope Tshwane, Africa.Setting: Data were collected nine within Tshwane Metropolitan Municipality.Methods: quantitative...
Copyright: 2012 London et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided original work properly cited.
HIV-1 subtype C viruses elicit potent but highly type-specific neutralizing antibodies within the first year of infection. In order to determine specificity and evolution these autologous antibodies, we examined neutralization escape in four individuals infected with from CAPRISA 002 cohort Durban, South Africa. Early responses recognized a very limited number epitopes, that recognize new epitopes evolving sequentially. addition, only two regions envelope were targeted by suggesting there...