A5007777158- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Insect symbiosis and bacterial influences
- Helminth infection and control
- Mosquito-borne diseases and control
- Plant Virus Research Studies
- Parasites and Host Interactions
- Biochemical and Molecular Research
- Parasitic Infections and Diagnostics
- Malaria Research and Control
- Insect-Plant Interactions and Control
- Vector-borne infectious diseases
- Parasite Biology and Host Interactions
- Synthesis and Biological Evaluation
- Insect Resistance and Genetics
- Evolution and Genetic Dynamics
- Protist diversity and phylogeny
- Environmental DNA in Biodiversity Studies
- Cardiomyopathy and Myosin Studies
- Toxin Mechanisms and Immunotoxins
- Bacteriophages and microbial interactions
- Mollusks and Parasites Studies
- Bat Biology and Ecology Studies
- Genetic diversity and population structure
- Lysosomal Storage Disorders Research
Institut de Recherche pour le Développement
2013-2024
Interactions hotes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides
2016-2024
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2014-2024
Université de Montpellier
1995-2024
Biologie et Génétique des Interactions Plante-Parasite
2014-2020
Agropolis International
2001-2016
Maladies Infectieuses et Vecteurs: Écologie, Génétique, Évolution et Contrôle
1999-2014
Centre National de la Recherche Scientifique
2004-2014
Institut de Recherche pour le Développement
2010-2013
Institut de Recherche pour le Développement
2003-2013
We have assayed genetic polymorphisms in several species of parasitic protozoa by means random amplified polymorphic DNA (RAPD). One goal was to ascertain the suitability RAPD markers for investigating and evolutionary problems, particularly organisms, such as protozoa, unsuitable traditional methods analysis. Another test certain hypotheses concerning Trypanosoma cruzi, other that been established multilocus enzyme electrophoresis. The results corroborate hypothesis population structure T....
Abstract Genetic variation in Trypanosoma cruzi is likely a key determinant transmission and pathogenesis of Chagas disease. We have examined nine loci as markers for the extant T. strains. Four distinct alleles were found each locus, corresponding to sequence classes present homozygous discrete typing units (DTUs) I, IIa, IIb, IIc. The DTUs IIa IIc showed spectrum polymorphism ranging from DTU I-like IIb-like, addition DTU-specific variation. IId IIe indistinguishable, showing homozygosity...
A set of 434 Trypanosoma cruzi stocks from a wide ecogeographical range was analysed by Multilocus Enzyme Electrophoresis for 22 genetic loci. Strong linkage disequilibrium, not associated with geographical distance, and 2 main clusters each considerably heterogeneous, observed. These results support the hypotheses previously proposed that T. natural populations are composed highly diversified clones distributed into phylogenetic lineages: lineage 1, most ubiquitous in endemic area, more...
The benznidazole (BZ) and itraconazole (ITC) susceptibilities of a standard set Trypanosoma cruzi natural stocks were evaluated during the acute phase chronic experimental chagasic infection in BALB/c mice. Twenty laboratory-cloned representative total phylogenetic diversity T. cruzi, including genotypes 20 19 (T. I) 39 32 II), analyzed. Our results demonstrate important differences among that could be pointed out as markers biological behavior. Members I group highly resistant to both BZ...
Abstract Studies have characterized Trypanosoma cruzi from parasite-endemic regions. With new human cases, increasing numbers of veterinary and influx potentially infected immigrants, understanding the ecology this organism in United States is imperative. We used a classic typing scheme to determine lineage 107 isolates various hosts.
ABSTRACT Randomly amplified polymorphic DNA (RAPD) has emerged as an effective genetic marker for analysis of Trypanosoma cruzi population variability. This method been used to study the variability Mexican T. stocks and relate these results previous classifications. High clonal diversity was observed among populations: 24 RAPD types were scored 56 analyzed. Only two (3.6%) belonged II lineage, while all others I. The robustness clusters statistically highly significant. I formed a...
Trypanosoma cruzi, the causative agent of Chagas disease, is a multiclonal parasite with high levels genetic diversity and broad host geographic ranges. Molecular characterization South American isolates T. cruzi has demonstrated homologous recombination nuclear hybridization, as well presence 6 main clusters or "discrete typing units" (DTUs). Few studies have extensively investigated such exchange events in North isolates. In current study, we genetically characterized over 50 US from...
Trypanosoma cruzi , the agent of Chagas' disease, exhibits considerable biological variability. Moreover, it has been postulated that populations this protozoan are subdivided into natural clones, which can be separated from each other by levels evolutionary divergence. The authors have proposed long-term clonal evolution may a profound impact on diversity. In order to test hypothesis, 16 T. stocks representing 3 major genotypes parasite were analysed for 8 different in vitro parameters....
In the field of parasitology, isozymes are mainly used to characterize and distinguish "strains." For such a difficult purpose, isozyme data definitely have be analyzed in genetic terms. Relying on purely empirical, phenetic reading gels amounts missing about 90% information that could derived from them. If correctly analyzed, electrophoresis provides an irreplaceable tool population genetics, yields cannot obtained by more "fashionable" techniques.
We performed a phylogenetic character mapping on 26 stocks of Trypanosoma cruzi, the parasite responsible for Chagas disease, and 2 sister taxon T. cruzi marinkellei to test possible associations between cruzi-subspecific diversity levels protein expression, as examined by proteomic analysis mass spectrometry. observed high level correlation (P < 10(-4)) genetic distance, established multilocus enzyme electrophoresis, dissimilarities estimated Euclidian distances. Several proteins were found...