A5050465176- Protein Structure and Dynamics
- SARS-CoV-2 and COVID-19 Research
- Computational Drug Discovery Methods
- Enzyme Production and Characterization
- Genetics, Bioinformatics, and Biomedical Research
- Peptidase Inhibition and Analysis
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Various Chemistry Research Topics
- Biochemical and Molecular Research
- Lipid Membrane Structure and Behavior
- vaccines and immunoinformatics approaches
- Innovative Teaching Methods
- Studies on Chitinases and Chitosanases
- Mass Spectrometry Techniques and Applications
- Protease and Inhibitor Mechanisms
- Plant and Biological Electrophysiology Studies
- Photoreceptor and optogenetics research
- Connexins and lens biology
- RNA regulation and disease
- COVID-19 Clinical Research Studies
- Click Chemistry and Applications
- Protein Hydrolysis and Bioactive Peptides
- Analytical Chemistry and Chromatography
- Animal Virus Infections Studies
University of California, Irvine
2020-2024
UC Irvine Health
2024
The SARS-CoV-2 main protease (Mpro) is essential to viral replication and cleaves highly specific substrate sequences, making it an obvious target for inhibitor design. However, as any virus, subject constant neutral drift selection pressure, with new Mpro mutations arising over time. Identification structural characterization of variants thus critical robust Here we report sequence analysis, structure predictions, molecular modeling seventy-nine variants, constituting all clinically...
Cataract disease is strongly associated with progressively accumulating oxidative damage to the extremely long-lived crystallin proteins of lens. Cysteine oxidation affects folding, interactions, and light-scattering aggregation especially due formation disulfide bridges. Minimizing crucial for lifelong lens transparency, so one might expect ubiquitous superfamilies (α βγ) contain little cysteine. Yet, Cys content γ-crystallins well above average human proteins. We review literature relevant...
The main protease of SARS-CoV-2 (M
ABSTRACT Specifications grading is a student-centered assessment method that enables flexibility and opportunities for revision. Here, we describe the first known full implementation of specifications in an upper-division chemical biology course. Due to rapid development relevant knowledge this discipline, overarching goal class prepare students interpret communicate about current research. In past, conventional points-based made it challenging ensure satisfactory standards student work were...
The Droserasins, aspartic proteases from the carnivorous plant Drosera capensis, contain a 100-residue plant-specific insert (PSI) that is post-translationally cleaved and independently acts as an antimicrobial peptide. PSIs are of interest not only for their inhibition microbial growth, but also because they modify size lipid vesicles strongly interact with biological membranes. may therefore be useful modulating systems in NMR studies membrane proteins. Here we present expression...
Prolyl oligopeptidases from psychrophilic, mesophilic, and thermophilic organisms found in a range of natural environments are studied using combination protein structure prediction, atomistic molecular dynamics, trajectory analysis to determine how the S9 protease family adapts extreme thermal conditions. We compare our results with hypotheses literature regarding structural adaptations that allow proteins maintain function at temperatures, we find that, case prolyl oligopeptidases, only...
The SARS-CoV-2 main protease (M pro ) is essential to viral replication and cleaves highly specific substrate sequences, making it an obvious target for inhibitor design. However, as any virus, subject constant selection pressure, with new M mutations arising over time. Identification structural characterization of variants thus critical robust Here we report sequence analysis, structure predictions, molecular modeling seventy-nine variants, constituting all clinically observed in this...
Understanding the molecular adaptations of organisms to extreme environments requires a comparative analysis protein structure, function, and dynamics across species found in different environmental conditions. Computational studies can be particularly useful this pursuit, allowing exploratory large numbers proteins under thermal chemical conditions that would infeasible carry out experimentally. Here, we perform such study MEROPS family S11, S12, S13 proteases from psychophilic, mesophilic,...
ABSTRACT A major challenge for science educators is teaching foundational concepts while introducing their students to current research. Here we describe an active learning module developed teach protein structure fundamentals supporting ongoing research in enzyme discovery. It can be readily implemented both entry-level and upper-division college biochemistry or biophysics courses. Preactivity lectures introduced of secondary provided context the projects, a homework assignment familiarized...
Abstract A major challenge for science educators is teaching foundational concepts while introducing their students to current research. Here we describe an active learning module developed teach protein structure fundamentals supporting ongoing research in enzyme discovery. It can be readily implemented both entry-level and upper-division college biochemistry or biophysics courses. Pre-activity lectures introduced of secondary provided context the projects, a homework assignment...