A5080351511- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Protein Degradation and Inhibitors
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- 3D Printing in Biomedical Research
- Connective Tissue Growth Factor Research
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
- Liver physiology and pathology
- Asthma and respiratory diseases
- Mitochondrial Function and Pathology
- Renal and related cancers
- Chronic Obstructive Pulmonary Disease (COPD) Research
- SARS-CoV-2 detection and testing
- Genetic Neurodegenerative Diseases
- Extracellular vesicles in disease
- Biomarkers in Disease Mechanisms
- Cancer-related molecular mechanisms research
- Chromatin Remodeling and Cancer
Koç University
2016-2025
Imperial College London
2016
NIHR Royal Brompton Cardiovascular Biomedical Research Unit
2016
Organoid technologies have become a powerful emerging tool to model liver diseases, for drug screening, and personalized treatments. These applications are, however, limited in their capacity generate functional hepatocytes reproducible efficient manner. Here, we generated characterized the hepatic organoid (eHEPO) culture system using human induced pluripotent stem cell (iPSC)-derived EpCAM-positive endodermal cells as an intermediate. eHEPOs can be produced within 2 weeks expanded long...
Cell-autonomous barriers to reprogramming somatic cells into induced pluripotent stem (iPSCs) remain poorly understood. Using a focused CRISPR-Cas9 screen, we identified Ubiquitin-specific peptidase 22 (USP22) as key chromatin-based barrier human iPSC derivation. Suppression of USP22 significantly enhances efficiency. Surprisingly, this effect is likely be independent USP22's deubiquitinase activity or its association with the SAGA complex, shown through module-specific knockouts, and...
Epigenetic reprogramming to pluripotency requires extensive remodeling of chromatin landscapes silence existing cell-type-specific genes and activate genes. ATP-dependent complexes are important regulators structure gene expression; however, the role recently identified Bromodomain-containing protein 9 (BRD9) associated non-canonical BRG1-associated factors (ncBAF) complex in remains unknown. Here, we show that genetic or chemical inhibition BRD9, as well ncBAF subunit GLTSCR1, but not...
Abstract Background The histone H3 lysine 79 (H3K79) methyltransferase DOT1L is a key chromatin-based barrier to somatic cell reprogramming. However, the mechanisms by which safeguards identity and somatic-specific transcriptional programs remain unknown. Results We employed proteomic approach using proximity-based labeling identify DOT1L-interacting proteins investigated their effects on Among interactors, suppression of AF10 ( MLLT10 ) via RNA interference or CRISPR/Cas9, significantly...
Purpose: Over the past three years, extensive research has been dedicated to understanding and combating COVID-19. Targeting interaction between SARS-CoV-2 Spike protein ACE2 receptor emerged as a promising therapeutic strategy against SARS-CoV-2. This study aimed develop ACE2-coated virus-like particles (ACE2-VLPs), which can be utilized prevent viral entry into host cells efficiently neutralize virus. Methods: Virus-like were generated through utilization of packaging plasmid in...
Friedreich's ataxia (FRDA, OMIM#229300) is the most common hereditary ataxia, resulting from reduction of frataxin protein levels due to expansion GAA repeats in first intron FXN gene. Why triplet repeat causes a decrease Frataxin not entirely known. Generation effective FRDA disease models crucial for answering questions regarding pathophysiology this disease. There have been considerable efforts generate vitro and vivo FRDA. In perspective article, we highlight studies conducted using...
Summary The histone H3 lysine 79 (H3K79) methyltransferase DOT1L is a key chromatin-based barrier to somatic cell reprogramming. However, the mechanisms by which safeguards identity and somatic-specific transcriptional programs remain unknown. Here, we employed proteomic approach using proximity-based labeling identify DOT1L-interacting proteins investigated their effects on Among interactors, suppression of AF10 (MLLT10) via RNA interference or CRISPR/Cas9, significantly increases...
Abstract Epigenetic reprogramming requires extensive remodeling of chromatin landscapes to silence cell-type specific gene expression programs. ATP-dependent chromatin-remodeling complexes are important regulators structure and expression; however, the role Bromodomain-containing protein 9 (BRD9) associated ncBAF (non-canonical BRG1-associated factors) complex in remains unknown. Here, we show that genetic suppression BRD9 as well subunit GLTSCR1, but not closely related BRD7, increase...
Glioblastoma multiforme (GBM) is the most common and aggressive type of gliomas with a mean survival 1 year after diagnosis. A major obstacle in treating GBMs extensive tumor cell infiltration into surrounding brain. Despite resection combined therapy, recurrence occurs vicinity margin due to individual cells that dispersed out primary tumor, therefore; developing novel therapies target dispersal high priority. The goal this project identify genes are differentially regulated during GBM...
Abstract A large body of research accumulated over the past three years dedicated to our understanding and fighting COVID-19. Blocking interaction between SARS-CoV-2 Spike ACE2 receptor has been considered an effective strategy as anti-SARS-CoV-2 therapeutics. In this study, we developed ACE2-coated virus-like particles (ACE2-VLPs), which can be utilized prevent viral entry into host cells efficiently neutralize virus. These ACE2-VLPs exhibited high neutralization capacity even when applied...