A5066359477- Forensic Toxicology and Drug Analysis
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Cannabis and Cannabinoid Research
- Alcohol Consumption and Health Effects
- Advanced Chemical Sensor Technologies
- Olfactory and Sensory Function Studies
- Pesticide Residue Analysis and Safety
- Psychedelics and Drug Studies
- Analytical Methods in Pharmaceuticals
- Analytical chemistry methods development
- Neuroscience and Neuropharmacology Research
- Plant biochemistry and biosynthesis
- Lipid metabolism and biosynthesis
- Genetic factors in colorectal cancer
- Analytical Chemistry and Chromatography
- Antibiotics Pharmacokinetics and Efficacy
- Venomous Animal Envenomation and Studies
- Cholesterol and Lipid Metabolism
- Pharmacological Effects and Assays
- Forensic Fingerprint Detection Methods
- Diverticular Disease and Complications
- Cancer survivorship and care
- Colorectal Cancer Screening and Detection
- Paraquat toxicity studies and treatments
Showa University
1997-2025
Nagoya University
2014-2019
Shimadzu (Japan)
2011-2014
Florida International University
2007-2012
The University of Tokyo
1991-1995
Abstract: Previous work has demonstrated the ability to differentiate individuals based on analysis of human scent hand odor chemicals. In this paper, a range forensic biological specimens are shown also have upon volatile organic compounds (VOCs) present. Human VOC profiles from odor, oral fluid, breath, blood, and urine 31 were analyzed by solid‐phase microextraction–gas chromatography–mass spectrometry (SPME‐GC‐MS) combined methods chromatogram comparison, Spearman rank correlation...
Despite the implementation of a new blanket scheduling system in 2013, psychoactive substance (NPS) abuse remains serious social concern Japan. We present fatal intoxication case involving 5F–ADB (methyl 2‐[1‐(5‐fluoropentyl)‐1 H –indazole‐3‐carboxamido]‐3,3‐dimethylbutanoate) and diphenidine. Postmortem blood screening by liquid chromatography/quadrupole time‐of‐flight mass spectrometry (LC/Q‐TOFMS) information‐dependent acquisition mode only detected Further urinary using an in‐house...
Abstract Herbicide poisoning commonly involves both paraquat and diquat (DQ); DQ alone is less frequently reported, especially rare in Japan. We present a case of fatal after attempted suicide by ingesting dibromide, requiring intensive care including hemodialysis (HD). Toxicological profiles DQ, metabolites, bromide ion serum were investigated relative to the course treatment. Quantitative analyses carried out liquid chromatography/tandem mass spectrometry (LC-MS/MS) for its oxidative...
Probe electrospray ionization (PESI) is a recently developed technique that enables the direct detection of endogenous compounds like metabolites without sample preparation. In this study, we have demonstrated first combination use PESI with triple quadrupole tandem mass spectrometry (MS/MS), which was then applied to intact metabolite analysis mice liver, achieving 26 including amino acids, organic and sugars. To investigate its practicality, metabolic profiles control CCl4-induced acute...
Like many new designer drugs of abuse, synthetic cannabinoids (SC) have structural or positional isomers which may not all be regulated under law. Differences in acute toxicity exist between impose further burden the fields forensic toxicology, medicine and legislation. Isomer differentiation therefore becomes crucial from these standpoints as continuously emerge with just minor modifications to their preexisting analogs. The aim this study was differentiate JWH-081. Purchased standard...
Recent improvements in ambient ionization techniques combined with mass spectrometry has enabled to achieve real-time monitoring of analytes interest, even for biogenic molecules living animals. Here, we demonstrate a newly developed system vivo metabolites mouse brain. It consists semiautomated manipulation and unique probe electrospray unit, which uses an extremely thin solid needle (tip dia.: 700 nm) direct sampling ionization, coupled conventional tandem spectrometer. The successfully...
A cDNA encoding rat 2, 3-oxidosqualene : lanosterol cyclase, the enzyme responsible for complex cyclization/rearrangement step in sterol biosynthesis, was cloned by extensive application of PCRs. Oligonucleotide primers were designed sense and anti-sense directions corresponding to internal peptide sequences purified enzyme. Successive PCRs with all possible combinations these yielded a 178-bp fragment, based on its sequence full nucleotide obtained "rapid amplification ends"(RACE) method.
Squalene-2, 3-epoxide : lanosterol cyclase was purified from rat liver in five steps as a soluble and homogeneous protein. The enzyme showed single band on SDS-polyacrylamide gel electrophoresis with molecular weight of 75 kD. In its native state it behaved homo-dimer. isoelectric point 5.5 the apparent Km value for (&3S)-squalene-epoxide 55 μM were estimated cyclase.
Iron oxide nanoparticle (NP)-coated target plates were employed for the direct detection and analysis of low molecular weight lipids by laser desorption/ionization (LDI) mass spectrometry (MS). We have demonstrated that use iron NP-coated provides a simple, direct, rapid method lipid standards epidermal surface without any cumbersome sample pretreatment as well spectra are free background matrix peaks. Lipid (1-stearoyl-sn-glycero-3-phosphocholine, 1,2-dioleoyl-sn-glycerol,...
One of the many issues designer drugs abuse like synthetic cannabinoids (SCs) such as JWH-018 is that details on their metabolism has yet to be fully elucidated and multiple metabolites exist. The presence isomeric compounds poses further challenges in identification. Our group previously shown effectiveness gas chromatography-electron ionization-tandem mass spectrometry (GC-EI-MS/MS) spectrometric differentiation positional isomers naphthoylindole-type SC JWH-081, speculated same approach...
BACKGROUND Liver metastases are found in 10% of primary colorectal malignancies, and they affects the prognosis patients with carcinoma. The authors investigated DNA copy number aberrations by using comparative genomic hybridization (CGH) ploidy alterations flow cytometry (FCM) carcinoma (primary tumors). To determine whether there characteristic that contribute to liver metastasis, cytogenetic were examined CGH FCM. METHODS analyzed 35 tumors, including 16 tumors RESULTS Increases numbers...
In this study, we describe a rapid gas chromatography-tandem mass spectrometry (GC-MS/MS) analytical method that allows comprehensive detection and structural elucidation of synthetic cathinone-type designer drugs. Our proposed consists three simultaneous procedures: 1) selective the carbonyl group characteristic to each cathinone examined via selected reaction monitoring (SRM); determination both 2) iminium cations 3) substituted benzoyl generated α-cleavage their corresponding amines...