A5046033039- T-cell and B-cell Immunology
- Renal Transplantation Outcomes and Treatments
- Pancreatic function and diabetes
- Xenotransplantation and immune response
- Diabetes and associated disorders
- Organ Transplantation Techniques and Outcomes
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Organ and Tissue Transplantation Research
- Toxin Mechanisms and Immunotoxins
- Transplantation: Methods and Outcomes
- Hematopoietic Stem Cell Transplantation
- Diabetes Management and Research
- Cell death mechanisms and regulation
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Virus-based gene therapy research
- Cannabis and Cannabinoid Research
- CAR-T cell therapy research
- Mesenchymal stem cell research
- Renal Diseases and Glomerulopathies
- Cell Adhesion Molecules Research
- Blood groups and transfusion
Helmholtz Zentrum München
2021-2022
Ludwig-Maximilians-Universität München
2021-2022
Metabolism and Renal Physiology
2019
Praxis (United States)
2015
University of Alabama at Birmingham
1999-2009
Bristol-Myers Squibb (United States)
1993-2008
National Institutes of Health
1999-2008
University of Alabama
1999-2007
Indiana University – Purdue University Indianapolis
2006
National Institute of Mental Health
2006
Owing to several recent developments, the cultivability of epidermal keratinocytes, particularly those human, has been greatly improved. Under conditions used, single cultured cells generate stratified colonies that ultimately fuse and form an epithelium is reasonable approximation epidermis. It will be shown here large amounts can generated from a small piece epidermis in short time. We wish bring attention surgeons cell biologists possibility using culture-grown derived same individual...
The potent immunosuppressive action of rabbit antithymocyte globulin (RATG) in allotransplant recipients has been recognized for many years. Some the antibody specificities and immunoregulatory effects RATG have described, but a comprehensive definition components not reported previously. In this study, we identified 23 that are consistent among different clinical batches represent major RATG. These were defined by immunoprecipitation/gel electrophoresis also blocking/flow cytometry methods....
Objective To examine the possibility of reducing ischemia-reperfusion injury (I/R injury) to mouse liver by in vivo adenovirus-mediated gene transfer antiapoptotic human Bcl-2 gene. Summary Background Data Ischemia-reperfusion has been demonstrated a number clinically relevant diseases such as myocardial infarction, cerebrovascular disease, sepsis, peripheral vascular and organ transplantation. In this regard, apoptosis plays central role. Methods Normal C57BL/6 mice were used. An adenovirus...
Background. Day of transplant T cell depletion with anti-CD3 immunotoxin or F(Ab)2 induces stable tolerance to renal allografts in rhesus monkeys given 15-deoxyspergualin (DSG), a NF-κB inhibitor that suppresses proinflammatory cytokine (PC) production. Because PC and are involved dendritic (DC) maturation, we asked if impaired DC maturation Th2-type deviation might be related the synergistic effect DSG this novel model. Methods. Immunosuppression was initiated 4 hr before transplanting...
THOMAS, JUDITH M.; CARVER, F. MELINDA; KASTEN-JOLLY, JANE; HAISCH, CARL E.; REBELLATO, LORITA GROSS, ULRIKE; VORE, STEPHEN J.; FRANCIS T. Author Information
A major challenge in clinical transplantation today is to design a practical and effective protocol for tolerance induction compatible with cadaver organ transplantation. preclinical rhesus monkey kidney allograft model using immediate peritransplant anti-CD3 immunotoxin (anti-CD3-IT) donor bone marrow (DBM) shown here induce operational prolonged graft survival the absence of chronic immunosuppressive drugs. Bone harvested from was depleted mature alloantigen-presenting cells T by removing...
Root-knot nematodes and rhizobia establish interactions with roots characterized by the de novo induction of host structures, termed giant cells nodules, respectively. Two transcription regulators, PHAN KNOX, required for establishment meristems were previously shown to be expressed in tomato cells. We isolated orthologues KNOX (Mt-phan Mt-knox-1) from model legume Medicago truncatula, established spatial distribution their expression situ. confirmed that Mt-phan Mt-knox-1 are lateral root...
This report extends previous studies demonstrating that prolonged acceptance of incompatible kidney allo-grafts in rhesus monkeys can be achieved by a short recipient rabbit antithymocyte globulin (RATG) treatment course followed donor bone marrow infusion on day 12 without requirement for chronic immune-suppression. Serial antilymphocyte cyctotoxic antibody recipients' sera following RATG injections showed pan-lymphocyte-reactive present until 10 posttransplant. On days 11 and 12, was no...
Isolated pancreatic islet transplantation is a promising alternative to conventional insulin-dependent diabetes treatment but not yet practical clinical therapy. In the first few days after transplantation, substantial donor dysfunction and apoptosis commonly occur. Islet has been documented extracellular matrix disruption exposure proinflammatory cytokines, during hypoxia before revascularization rejection. These studies show that targeting pathway by adenoviral-mediated gene transfer of...
The recent focus on islet transplantation as primary therapy for type 1 diabetes has heightened interest in the reversal of preclinical models using minimal immunosuppression. Here, we demonstrated a rhesus model consistent all measured glycemic patterns streptozotocin-induced diabetes. used single-donor with induction operational tolerance. term “operational tolerance” is to indicate durable survival major histocompatibility complex (MHC)-mismatched allografts without maintenance...
In cultures made from disaggregated human epidermal cells, growth to a confluent cell layer is followed by the emergence of patterns resembling those dermatoglyphs. These reflect intrinsic properties keratinocytes. vivo, only epidermis volar surfaces forms patterns, but in culture, are formed cells other sites as well. Patterns develop process movement which first produces ridges and then curves into figures increasing complexity, ultimately whorls.
Background. Isolated pancreatic islet transplantation (IPITx) is an attractive alternative for treatment of insulin-dependent diabetes mellitus (IDDM). However, IPITx has been difficult to implement clinically because islets frequently fail function, have a high incidence rejection, and are susceptible autoimmune recurrence damage by chronic immunosuppressive therapy. Tolerance induction may be rational approach resolve several these limitations. Because anti-CD3 immunotoxin (IT) successful...
Tolerance induction can prevent acute kidney allograft rejection without chronic immunosuppression. It is uncertain whether specific tolerance nephropathy (CAN), which involves both nonimmune and immune injury. This report provides evidence that immunologically tolerant macaques, induced with immunotoxin deoxyspergualin, developed neither nor CAN. Long survivors, bearing MHC-mismatched grafts immunosuppression for 0.8 to 3.4 years, exhibited general competence donor-specific T B cell no...
Background. Tolerance is gaining momentum as an approach to reduce lifelong immunosuppressive therapy while improving transplant longevity. Anti-CD3 immunotoxin (IT), FN18-CRM9, has potential induce tolerance owing its exceptional ability deplete sessile lymph node T cells. However, if initiated at the time of transplantation, α-CD3-IT alone elicits a proinflammatory cytokine response, precluding establishment tolerance. Methods. Four groups rhesus monkeys received kidney allografts and...
Cell transplantation is a promising approach for the reconstruction of neuronal circuits after brain damage. Transplanted neurons integrate with remarkable specificity into circuitries mouse cerebral cortex affected by ablation. However, it remains unclear how perform in local environment undergoing reactive gliosis, inflammation, macrophage infiltration, and scar formation, as traumatic injury (TBI). To elucidate this, we transplanted cells from embryonic TBI-injured, inflamed-only, or...
Background. Liver function after transplantation is determined by the quality of donor organ and influences preservation, flush, reperfusion injury. In this regard, cell death (apoptosis) plays an important role in preservation rejection. Therefore, we examined possibility genetic modification liver graft with a recombinant adenovirus vector encoding Bcl-2 gene to reduce apoptosis during time. Methods. grafts from C57Bl/6 mice were procured preserved using standard techniques. A replication...
African-American race is associated with an increased risk of allograft loss, suggesting that patients may form immunologically higher group. Previously, we demonstrated immune cell costimulatory molecule expression significantly in African-Americans than Caucasians. Polymorphic variations the genes for cytokines have been a number immunological conditions, and transplant rejection. This study was performed to determine distribution, Caucasian renal recipients, single nucleotide...