A5103136904- Genetic Mapping and Diversity in Plants and Animals
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Forest Insect Ecology and Management
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Wheat and Barley Genetics and Pathology
- Plant Reproductive Biology
- Genetic and phenotypic traits in livestock
- Genetics and Plant Breeding
- Plant Disease Resistance and Genetics
- Genetic diversity and population structure
- Photosynthetic Processes and Mechanisms
- Insect and Pesticide Research
- Genomics, phytochemicals, and oxidative stress
- Plant pathogens and resistance mechanisms
- Forest Ecology and Biodiversity Studies
- Insect-Plant Interactions and Control
- Forest ecology and management
- Plant Pathogens and Resistance
- Plant Taxonomy and Phylogenetics
- Plant Gene Expression Analysis
- Plant Surface Properties and Treatments
- Ziziphus Jujuba Studies and Applications
- Hemiptera Insect Studies
John Innes Centre
1999-2024
Norwich Research Park
1996-2024
University of Edinburgh
1986
Rocky Mountain Research Station
1977-1983
US Forest Service
1971-1973
A potentially serious disadvantage of association mapping is the fact that marker-trait associations may arise from confounding population structure as well linkage to causative polymorphisms. Using genome-wide marker data, we have previously demonstrated problem can be severe in a global sample 95 Arabidopsis thaliana accessions, and established methods for controlling are generally insufficient. Here, use same together with number flowering-related phenotypes data-perturbation simulations...
Recombinant inbred (RI) lines of Arabidopsis thaliana (Arabidopsis) have been generated from a cross between the ecotypes Landsberg erecta and Columbia. Progeny 300 individual F 2 seedlings were taken by single seed descent to 8 generation. Sixty‐seven loci, scored using 64 RFLP probes one phenotypic marker, chosen at approximately 20 cM intervals two previously published maps, mapped 100 these RI lines. More than 500 other new loci are currently being several groups. These thus provide...
There is currently tremendous interest in the possibility of using genome-wide association mapping to identify genes responsible for natural variation, particularly human disease susceptibility. The model plant Arabidopsis thaliana many ways an ideal candidate such studies, because it a highly selfing hermaphrodite. As result, species largely exists as collection naturally occurring inbred lines, or accessions, which can be genotyped once and phenotyped repeatedly. Furthermore, linkage...
Abstract Harnessing genetic diversity in major staple crops through the development of new breeding capabilities is essential to ensure food security 1 . Here we examined and phenotypic A. E. Watkins landrace collection 2 bread wheat ( Triticum aestivum ), a global cereal, by whole-genome re-sequencing 827 landraces 208 modern cultivars in-depth field evaluation spanning decade. We found that are derived from two seven ancestral groups maintain very long-range haplotype integrity. The...
Allelic variation at the FRI (FRIGIDA) and FLC (FLOWERING LOCUS C) loci are major determinants of flowering time in Arabidopsis accessions. Dominant alleles confer a vernalization requirement causing plants to overwinter vegetatively. Many early accessions carry loss-of-function fri containing one two deletions. However, some categorized as types do not these deletion alleles. We have analyzed molecular basis earliness five accessions: Cvi, Shakhdara, Wil-2, Kondara, Kz-9. The Cvi allele...
A new collection of 129 Arabidopsis thaliana RFLP markers has been established based upon DNA fragments cloned in the pUC119 plasmid vector and insert end sequences P1 clones. Dominant/null alleles affecting low‐copy number account for nine mapped polymorphisms, suggesting that deletions are not rare A. . Recombinant inbred (RI) lines were used mapping these marker loci. RI line‐based allows integration this set with previously reported as well any future using replenishable resource. These...
The detection of footprints natural selection in genetic polymorphism data is fundamental to understanding the basis adaptation, and has important implications for human health. standard approach been reject neutrality favor if pattern variation at a candidate locus was significantly different from predictions neutral model. problem that model assumes more than just neutrality, it almost always possible explain using an alternative with complex demography. Today's wealth genomic data,...
ARC (accumulation and replication of chloroplasts) genes control different aspects the chloroplast division process in higher plants. In order to establish hierarchy provide evidence for their specific roles, double mutants were constructed between arc11, arc6, arc5, arc3 arc1 all combinations phenotypically analysed. arc11 is a new nuclear recessive mutant with 29 chloroplasts compared 120 wild type. All phenotypes are unambiguous. ARC1 down-regulates proplastid but on separate pathway from...
Summary The somatic and germinal activity of the maize transposable element, Ac , has been analysed in progeny 43 transformants A. thaliana using a streptomycin resistance assay to monitor excision. ability enabled, for first time, detailed analysis individual seedlings be made. effects T‐DNA copy number, generation, dosage at each locus, flanking sequences orientation element were compared. most striking observation was variability genotypically identical individuals poor penetrance...
Abstract Automated phenotyping technologies are capable of providing continuous and precise measurements traits that key to today’s crop research, breeding agronomic practices. In additional monitoring developmental changes, high-frequency high-precision phenotypic analysis can enable both accurate delineation the genotype-to-phenotype pathway identification genetic variation influencing environmental adaptation yield potential. Here, we present an automated scalable field platform called...
Peronospora parasitica causes downy mildew on crucifers. An isolate of P. (denoted NoCO2) was identified that infected Arabidopsis plants the land race Columbia (Col‐0) but not Landsberg erecta (La‐ er ). Segregation analysis F 2 derived from a La‐ x Col‐0 cross established resistance inherited as single locus, denoted RPP5 . Macroscopic and microscopic examinations inoculated cotyledons showed restriction fungal growth in accompanied by massive callose accumulation death plant cells direct...
Abstract Breeding crops resilient to climate change is urgently needed help ensure food security. A key challenge harness genetic diversity optimise adaptation, yield, stress resilience and nutrition. We examined the phenotypic of A.E. Watkins landrace collection bread wheat ( Triticum aestivum ), a major global cereal, through whole-genome re-sequencing (827 landraces 208 modern cultivars) in-depth field evaluation spanning decade. discovered that cultivars are derived from just two seven...
A pilot-scale transposon mutagenesis experiment using a modified autonomous Activator (Ac) element, Ac delta Nael, was carried out in Arabidopsis thaliana. Four different transformants carrying elements and defined genomic locations were used to generate 1000 plants approximately 500 independent germinal transposition events. These then selfed the families screened tissue culture soil for phenotypic mutants. Fifty segregated mutations their progeny. Preliminary Southern blot analysis of 29...
The nivea (niv) gene of Antirrhinum majus encodes chalcone synthase, an enzyme involved in synthesis anthocyanin pigments. nivrec:98 allele contains a single copy the transposon Tam3 inserted at niv locus. A large chromosomal rearrangement derived from this mutant has been shown to be flanked by two copies Tam3. In study, we compared sequences with their progenitor and concluded that is inversion resulting aberrant transposition occurring shortly after replication left both active...
Crop height (Ht), heading date (Hd), and grain yield (GY) are inter-related in wheat. Independent manipulation of each is important for adaptation performance. Validated quantitative trait loci (QTLs) all three co-locate on chromosome 3A the Avalon×Cadenza population, with increased Ht, Hd, GY contributed by Cadenza. We asked if these linked or pleiotropic effects using recombinant lines, showed that Ht Hd independent. The Chinese Spring equivalent to newly defined interval contained a gene...
There is currently tremendous interest in the possibility of using genome-wide association mapping to identify genes responsible for natural variation, particularly human disease susceptibility. The model plant Arabidopsis thaliana many ways an ideal candidate such studies, because it a highly selfing hermaphrodite. As result, species largely exists as collection naturally occurring inbred lines, or accessions, which can be genotyped once and phenotyped repeatedly. Furthermore, linkage...
Journal Article Refinement of the Lethal Trap Tree Method for Spruce Beetle Control Get access C. K. Lister, Lister Search other works by this author on: Oxford Academic PubMed Google Scholar J. M. Schmid, Schmid D. Minnemeyer, Minnemeyer R. H. Frye Economic Entomology, Volume 69, Issue 3, 1 June 1976, Pages 415–418, https://doi.org/10.1093/jee/69.3.415 Published: 01 1976 history Received: 02 October 1975
Tag1 was identified as a highly active endogenous transposable element in transgenic Arabidopsis thaliana Landsberg erecta plants carrying the maize Activator (Ac). Here, we describe experiments designed to determine basis for high activity of Tag1. The frequency transposition elements compared lines containing or lacking Ac transposase assess effect on activity. Three populations nontransgenic plants, including nontransformed regenerants, were also analyzed. level did not correlate with...
Journal Article Phloem Thickness in Lodgepole Pine: Its Relationship to Dwarf Mistletoe and Mountain Pine Beetle (Coleoptera: Scolytidae) Get access Frank G. Hawksworth, Hawksworth U.S. Department of Agriculture Forest Service, Rocky Range Experiment Station, Fort Collins, Colorado 80526 Search for other works by this author on: Oxford Academic PubMed Google Scholar C. Kendall Lister, Lister 2 USDA Region, Pest Management, Lakewood, CO 80225. Donn B. Cahill 3 Intermountain Boise, ID 83702....