A5047684055- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Biochemical and Molecular Research
- Click Chemistry and Applications
- Hepatitis C virus research
- Bacterial Genetics and Biotechnology
- Gene Regulatory Network Analysis
- Synthesis and biological activity
- Synthesis of Organic Compounds
- Synthesis of Indole Derivatives
- DNA and Nucleic Acid Chemistry
- Protein Structure and Dynamics
- HIV/AIDS Research and Interventions
- Cytomegalovirus and herpesvirus research
- Advanced biosensing and bioanalysis techniques
- Organic Chemistry Synthesis Methods
- Metal complexes synthesis and properties
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Cholesterol and Lipid Metabolism
- Chromatin Remodeling and Cancer
- Virus-based gene therapy research
- Cancer therapeutics and mechanisms
- Carbohydrate Chemistry and Synthesis
Centre National de la Recherche Scientifique
2004-2024
École Normale Supérieure - PSL
2024
Université Paris-Saclay
2023
Laboratoire de Biologie et Pharmacologie Appliquée
2004-2014
École Normale Supérieure Paris-Saclay
2004-2011
KU Leuven
2008
Laboratoire de Chimie Organique
2004-2008
Laboratoire d'Énergétique Moléculaire et Macroscopique, Combustion
2005
Rosmarinic acid was reacted with nitrite ions under acidic conditions to give 6'-nitro- and 6',6''-dinitrorosmarinic acids according the reaction time. Both compounds were active as HIV-1 integrase inhibitors at submicromolar level. They also inhibited viral replication in MT-4 cells modest similar selectivity indexes. The nitration of rosmarinic strongly improves anti-integrase inhibition antiviral activity without increasing cellular toxicity.
To replicate, human immunodeficiency virus, type 1 (HIV-1) needs to integrate a cDNA copy of its RNA genome into chromosome the host cell, step controlled by viral integrase (IN) protein. Viral integration involves participation several cellular proteins. SNF5/Ini1, subunit SWI/SNF chromatin remodeling complex, was first cofactor identified interact with IN. We report here that SNF5/Ini1 interferes early steps HIV-1 replication. Inhibition expression interference increases Using quantitative...
Initiation of DNA replication in
BackgroundHIV-1 integrase (IN) catalyses the retroviral integration process, removing two nucleotides from each long terminal repeat and inserting processed viral DNA into target DNA. It is widely assumed that strand transfer step has no sequence specificity. However, recently, it been reported by several groups sites display a preference for palindromic sequences, suggesting symmetry in may stabilise tetrameric organisation of IN synaptic complex.Methodology/Principal FindingsWe assessed...
The interactions with divalent cations of 4-phenyl-4-oxo-2-hydroxybuten-2-oic acid (benzoylpyruvic (BPA)), the pharmacophore HIV-1 integrase inhibitors, were investigated using spectroscopic tools. In absence enzyme, a 2:2 metal−ligand complex was characterized an intermetallic distance 4−6 Å. Molecular modeling allowed us to propose compatible structure for complex. BPA does not inhibit reactions catalyzed by IN, emphasizing importance aromatic ring substitution in antiviral activity.
Abstract Retroviral integration is central to viral persistence and pathogenesis, cancer as well host genome evolution. However, it unclear why appears essential for retrovirus production, especially given the abundance transcriptional potential of non-integrated genomes. The involvement retroviral endonuclease, also called integrase (IN), in replication steps apart from has been proposed, but usually considered be accessory. We observe here that a spumavirus family depends mainly on...
Gene expression in bacterial cells is dependent on a gene&prime s position along the genome, mainly because of effects neighbouring genes&prime expression, but also local activity nucleoid proteins, differing levels DNA supercoiling and changes gene copy number with growth rate phase. This genome dependence can be source specific regulation, however, some cases it necessary to have insulated from these effects. Escherichia coli express ribosomal RNA multiple operons found at...
A long-standing hypothesis sees DNA replication control in E. coli as a central cell cycle os-cillator at whose core is the DnaA protein. The consensus that activity of protein, which dependent on its nucleotide bound state, an effector initiation and sensor size. However, while several processes are known to regulate function cycle, oscillations expression ac-tivity have never been observed single level, their correlation with volume has yet be established. In this study, we measured...
Abstract ChemInform is a weekly Abstracting Service, delivering concise information at glance that was extracted from about 200 leading journals. To access of an article which published elsewhere, please select “Full Text” option. The original trackable via the “References”
Abstract ChemInform is a weekly Abstracting Service, delivering concise information at glance that was extracted from about 200 leading journals. To access of an article which published elsewhere, please select “Full Text” option. The original trackable via the “References”