A5056617349- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Plant Virus Research Studies
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Vector-Borne Animal Diseases
- Malaria Research and Control
- Insect symbiosis and bacterial influences
- HIV Research and Treatment
- Viral Infections and Outbreaks Research
- Neonatal and fetal brain pathology
- Viral Infections and Immunology Research
- Immunotherapy and Immune Responses
- Neuroinflammation and Neurodegeneration Mechanisms
- Virology and Viral Diseases
- Diabetes and associated disorders
- Vibrio bacteria research studies
- Hepatitis Viruses Studies and Epidemiology
- Cannabis and Cannabinoid Research
- Energy and Environment Impacts
- Studies on Chitinases and Chitosanases
- Vector-borne infectious diseases
- Polyamine Metabolism and Applications
- Plant and Fungal Interactions Research
- Antimicrobial Peptides and Activities
Institut Pasteur
2012-2023
Université Paris Cité
2022
Department of Virology
2012
National Institute of Allergy and Infectious Diseases
2006
National Institutes of Health
2004
Inserm
1997-2001
California Institute of Technology
1992-1996
Centre National de la Recherche Scientifique
1987-1990
Background A chikungunya virus outbreak of unprecedented magnitude is currently ongoing in Indian Ocean territories. In Réunion Island, this alphavirus has already infected about one-third the human population. The main clinical symptom disease a painful and invalidating poly-arthralgia. Besides arthralgic form, 123 patients with confirmed infection have developed severe signs, i.e., neurological signs or fulminant hepatitis. Methods Findings We report nearly complete genome sequence six...
ABSTRACT RNA viruses present an extraordinary threat to human health, given their sudden and unpredictable appearance, the potential for rapid spread among population, ability evolve resistance antiviral therapies. The recent emergence of chikungunya virus, Zika Ebola virus highlights struggles contain outbreaks. A significant hurdle is availability antivirals treat infected or protect at-risk populations. While several compounds show promise in vitro vivo , these outbreaks underscore need...
Highlights•Mouse embryonic brain slices sustain Zika and West Nile, but not Dengue-4, virus replication.•Zika virus, Nile exhibits a selective tropism of infection for neural stem cells.•Zika alters cell cycle progression cells.A outbreak in South America is currently responsible large burst microcephaly cases, congenital malformation characterized by reduced size. We describe here an assay to infect cultured mouse with as well other closely related flaviviruses demonstrated cause...
Abstract Arthropod-borne viruses pose a major threat to global public health. Thus, innovative strategies for their control and prevention are urgently needed. Here, we exploit the natural capacity of generate defective viral genomes (DVGs) detriment. While DVGs have been described most viruses, identifying which, if any, can be used as therapeutic agents remains challenge. We present combined experimental evolution computational approach triage DVG sequence space pinpoint fittest deletions,...
Identification and characterization of virus-host interactions are very important steps toward a better understanding the molecular mechanisms responsible for disease progression pathogenesis. To date, few cellular factors involved in life cycle flaviviruses, which human pathogens, have been described. In this study, we demonstrate crucial role class II Arf proteins (Arf4 Arf5) dengue flavivirus cycle. We show that simultaneous depletion Arf4 Arf5 blocks recombinant subviral particle...
We have isolated a 50-kDa mosquito protein that binds with high affinity to riboprobe representing the 3' end of minus strand Sindbis virus RNA. The has been used obtain cDNA clones encoding this sequenced and express in large amounts. Sequence comparisons make clear is homolog La autoantigen. N-terminal half shares considerable sequence identity human protein, rat recently identified Drosophila melanogaster homolog. There one stretch 100 amino acids domain which 48 residues are identical...
Forty-four nucleotides at the 5' terminus of genomic RNA Sindbis virus can form a stable stem-loop structure and have been shown previously to be important for viral replication. The formed by complement this sequence 3' end minus-strand has proposed promoter replication as such might bound in specific fashion proteins either cellular or origin. Short oligonucleotide probes (either 62 132 nucleotides) representing 3'-terminal minus strand were prepared. When added extracts from infected...
The two-signal model states that activation of naive T cells requires a signal 1 stimulus through the TCR and co-stimulatory 2. By contrast, alone is sufficient for pre-activated cells. Recently, however, it has been shown under certain conditions can bypass requirement co-stimulation. For example, CD28-deficient mice, when immunized with lymphocytic choriomeningitis virus, mount vigorous cytotoxic lymphocyte response clear virus. As continuous effort to unravel mechanisms cell activation,...
Analysis of the RNAs present in BHK-21 cells infected with Germiston virus showed that transcripts from L and M segments have a size similar to their template, whereas two types complementary RNA are transcribed S segment. One, S1, is full-length "plus" strand (antigenome), other, S2, an incomplete plus which serves as mRNA for at least synthesis N protein virus-specific nonstructural polypeptide, p12. The 5' ends these appeared be identical 3' viral RNA. Our results suggest transcription...
Japanese encephalitis virus (JEV) membrane (M) protein plays important structural roles in the processes of fusion and maturation progeny during cellular infection. The M is anchored viral membrane, its ectodomain composed a flexible N-terminal loop perimembrane helix. In this study, we performed site-directed mutagenesis on residue 36 JEV showed that resulting mutation had little or no effect entry process but greatly affected assembly mammalian cells. Interestingly, mutant host-dependent...
The 3' end of Sindbis virus minus-sense RNA was tested for its ability to bind proteins in mosquito cell extracts, using labeled riboprobes that represented different parts this region. We found four domains the first 250 nucleotides could same 50- and 52-kDa proteins, three with high affinity one low affinity, whereas outside region did not these proteins. binding domain 60 nucleotides, which represents complement 5'-nontranslated region, second next third following fourth between 194 249...
Abstract CD8+ T cells are important effectors, as well regulators, of organ-specific autoimmunity. Compared with Tc1-type cells, Tc2 have impaired anti-viral and anti-tumor effector functions, although no data yet available on their pathogenic role in Our aim was to explore the autoreactive Tc1 autoimmune diabetes. We set up an adoptive transfer model which recipients were transgenic mice expressing influenza virus hemagglutinin (HA) specifically pancreatic β islet (rat insulin promoter-HA...
Using male and female RAG–/– mutant mice expressing TCR transgenes specific for MHC class I- or II-presented HY peptides, we performed quantitative phenotypic comparisons between the TCR+ lymphocytes present in lymphoid organs gut mucosa euthymic versus athymic (nude) animals. These suggest that only a minority of CD8α α + intraepithelial (IEL) transgenic originate from hematopoietic precursors acquiring wall, while majority these α+ IEL appear to be thymic origin (as were all β CD4+ any...
Infectious clones of West Nile virus (WNV) have previously been generated and used to decipher the role viral proteins in WNV virulence. The majority molecular obtained date derived from North American, Australian, or African isolates. Here, we describe construction an infectious cDNA clone a Mediterranean strain, IS-98-ST1. We characterized biological properties recovered recombinant cell culture mice. growth kinetics parental were similar Vero cells. Moreover, phenotype was...
West Nile virus (WNV) is a Flavivirus, which can cause febrile illness in humans that may progress to encephalitis. Like any other obligate intracellular pathogens, Flaviviruses hijack cellular protein functions as strategy for sustaining their life cycle. Many proteins display globular domain known PDZ interacts with PDZ-Binding Motifs (PBM) identified many viral proteins. Thus, PDZ-containing are common targets during infection. The non-structural 5 (NS5) from WNV provides both RNA cap...