A5059196144- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Microbial infections and disease research
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Animal Genetics and Reproduction
- Cancer therapeutics and mechanisms
- RNA Interference and Gene Delivery
- Nuclear Structure and Function
- Genomics and Phylogenetic Studies
- Toxoplasma gondii Research Studies
- Plant Virus Research Studies
- Herpesvirus Infections and Treatments
- Hemoglobinopathies and Related Disorders
- Bacterial Genetics and Biotechnology
- Cancer-related gene regulation
- Pneumonia and Respiratory Infections
- CRISPR and Genetic Engineering
- Vector-borne infectious diseases
Institute of Gene Biology
2016-2025
Lomonosov Moscow State University
2016-2025
Moscow State University
2008-2025
Genomics (United Kingdom)
2025
Russian Academy of Sciences
2007-2024
Technion – Israel Institute of Technology
2023
Institut Gustave Roussy
2011-2019
Laboratoire d'études sur les monothéismes
2012-2017
Université de Rennes
2016
Kurchatov Institute
2016
Recent advances enabled by the Hi-C technique have unraveled many principles of chromosomal folding that were subsequently linked to disease and gene regulation. In particular, revealed chromosomes animals are organized into topologically associating domains (TADs), evolutionary conserved compact chromatin influence expression. Mechanisms underlie partitioning genome TADs remain poorly understood. To explore TAD in Drosophila melanogaster , we performed poly(A) + RNA-seq four cell lines...
Abstract How the nuclear lamina (NL) impacts on global chromatin architecture is poorly understood. Here, we show that NL disruption in Drosophila S2 cells leads to compaction and repositioning from envelope. This increases density a fraction of topologically-associating domains (TADs) enriched active enhances interactions between inactive chromatin. Importantly, upon NL-associated TADs become more acetylated at histone H3 less compact, while background transcription derepressed. Two-colour...
The biological properties and special characteristics of the human T mycoplasmas have been reviewed summarized. are distinguished from all other known by their production urease, and, therefore, ability to hydrolyze urea. This singular property significantly sets apart members order Mycoplasmatales. In consideration this distinguishing property, it is reasonable propose establishing a new, separate genus in family Mycoplasmataceae which classify isolated man lower animals. name Ureaplasma...
DNA fragments containing replication origins (oriDNA) were isolated from a chicken erythroblast cell line by modified procedure of Zannis-Hadjopoulos et al. and studied in the renaturation reaction driven either total or nuclear matrix (nmDNA) same cells mature erythrocytes. We found that unique sequences nmDNA erythroblasts (5 kb long) represented specific subset constituting about quarter sequences, while erythrocyte 5 constitute only one tenth all are recovered among nmDNA. Virtually...
SUMMARY: Various Mycoplasma strains were found to differ in their sensitivity osmotic lysis. Three of the saprophytic laidlawii and a strain parasitic M. bovigenitalium readily lysed media low tonicity. Strains mycoides var. mycoides, capri, gallisepticum neurolyticum great extent resistant The lysis markedly decreased with ageing organisms. was more sensitive when grown cholesterol-free media.
Emotions coordinate our behavior and physiological states during survival-salient events pleasurable interactions. Even though we are often consciously aware of current emotional state, such as anger or happiness, the mechanisms giving ...Emotions felt in body, somatosensory feedback has been proposed to trigger conscious experiences. Here reveal maps bodily sensations associated with different emotions using a unique topographical self-report method. In ...
Razin , S. (University of Connecticut, Storrs), M. E. Tourtellotte, R. N. McElhaney, and J. D. Pollack . Influence lipid components Mycoplasma laidlawii membranes on osmotic fragility cells. Bacteriol. 91: 609–616. 1966.—Lipid composition could be significantly changed by variations in the growth medium. The effect these changes cells was studied. Cholesterol, incorporated into membrane from medium, had no significant fragility. Carotenoids, synthesized acetate, were likewise without effect....
Although the heat-stress response has been extensively studied for decades, very little is known about its effects on nucleic acids and acid-associated processes. This due to fact that research focused study of heat shock proteins factors (HSPs HSFs), their involvement in regulation transcription, protein homeostasis, etc. Recently, there some progress stress DNA integrity. In this review, we summarize discuss well-known potential mechanisms formation various stress-induced damage.
Abstract Liquid–liquid phase separation (LLPS) contributes to the spatial and functional segregation of molecular processes within cell nucleus. However, role played by LLPS in chromatin folding living cells remains unclear. Here, using stochastic optical reconstruction microscopy (STORM) Hi-C techniques, we studied effects 1,6-hexanediol (1,6-HD)-mediated disruption/modulation on higher-order organization cells. We found that 1,6-HD treatment caused enlargement nucleosome clutches their...
Abstract Mammalian and Drosophila genomes are partitioned into topologically associating domains (TADs). Although this partitioning has been reported to be functionally relevant, it is unclear whether TADs represent true physical units located at the same genomic positions in each cell nucleus or emerge as an average of numerous alternative chromatin folding patterns a population. Here, we use single-nucleus Hi-C technique construct high-resolution maps individual genomes. These demonstrate...
Abstract The first activation of gene expression during development (zygotic genome activation, ZGA) is accompanied by massive changes in chromosome organization. connection between these two processes remains unknown. Using Hi-C for zebrafish embryos, we found that folding starts establishing “fountains”, novel elements organization, emerging selectively at enhancers upon ZGA. polymer simulations, demonstrate fountains can emerge as sites targeted cohesin loading and require two-sided, yet...
It has been shown recently that apoptotic degradation of genomic DNA in mammalian cells starts by excision large fragments ranging size from 50 kilobases to more then 300 kilobases. Although it was suggested the above could represent chromosomal loops, supposition not supported direct experimental evidence. In present work, we have studied specificity nucleolar and euchromatic gene long-range fragmentation mouse human triggered undergo apoptosis either tumor necrosis factor or serum...