A5002526032- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- Plant Disease Resistance and Genetics
- Plant Pathogens and Resistance
- Mycotoxins in Agriculture and Food
- Genetic and Environmental Crop Studies
- Plant Pathogenic Bacteria Studies
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Plant pathogens and resistance mechanisms
- Plant tissue culture and regeneration
- Mycorrhizal Fungi and Plant Interactions
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Plant Reproductive Biology
- Genetics, Bioinformatics, and Biomedical Research
- Fungal Plant Pathogen Control
- Biofuel production and bioconversion
- Genetic Mapping and Diversity in Plants and Animals
- Microbial Metabolic Engineering and Bioproduction
- Agricultural pest management studies
- Polysaccharides and Plant Cell Walls
- Plant Genetic and Mutation Studies
- RNA modifications and cancer
China Agricultural University
2007-2025
Ministry of Agriculture and Rural Affairs
2021-2025
University of Saskatchewan
2009-2017
Agriculture and Agri-Food Canada
2017
Swift Current Research and Development Centre
2017
Saskatoon Medical Imaging
2011-2014
Jilin University
2006
Plant nucleotide-binding and leucine-rich repeat (NLR) receptors recognize avirulence effectors directly through their integrated domains (IDs) or indirectly via the effector-targeted proteins. Previous studies have succeeded in generating designer NLR with new recognition profiles by engineering IDs targeted proteins based on prior knowledge of interactions effectors. However, it is yet a challenge to design plant receptor capable recognizing that function unknown mechanisms. Several rice...
Fungal cell walls undergo continual remodeling that generates β-1,3-glucan fragments as products of endo-glycosyl hydrolases (GHs), which can be recognized pathogen-associated molecular patterns (PAMPs) and trigger plant immune responses. How fungal pathogens suppress those responses is often poorly understood. Here, we study mechanisms underlying the suppression β-1,3-glucan-triggered immunity by blast fungus Magnaporthe oryzae. We show an exo-β-1,3-glucanase GH17 family, named Ebg1,...
Abstract Some plant sensor nucleotide-binding leucine-rich repeat (NLR) receptors detect pathogen effectors through their integrated domains (IDs). Rice RGA5 NLR recognizes its corresponding AVR-Pia and AVR1-CO39 from the blast fungus Magnaporthe oryzae direct binding to heavy metal-associated (HMA) ID trigger RGA4 helper NLR-dependent resistance in rice. Here, we report a mutant of named HMA5 that confers complete transgenic rice plants M. strains expressing noncorresponding effector...
ABSTRACT The hemibiotrophic fungus Colletotrichum truncatum causes anthracnose disease on lentils and a few other grain legumes. It shows initial symptomless intracellular growth, where colonized host cells remain viable (biotrophy), then switches to necrotrophic killing the plant tissues. Here, we report novel effector gene, CtNUDIX , from C. that is exclusively expressed during late biotrophic phase (before switch necrotrophy) elicits hypersensitive response (HR)-like cell death in tobacco...
Maize (Zea mays) is one of the most important crops in world, but its yield and quality are seriously affected by diverse diseases. Identifying broad-spectrum resistance genes crucial for developing effective strategies to control disease maize. In a genome-wide study maize, we identified G-type lectin receptor kinase ZmLecRK1, as new protein against Pythium aphanidermatum, causal pathogens stalk rot Genetic analysis showed that specific ZmLecRK1 allele can confer multiple The cell death...
Rice blast caused by Pyricularia oryzae (syn., Magnaporthe oryzae) was one of the most destructive diseases rice throughout world. Genome assembly fundamental to genetic variation identification and critically impacted understanding its ability overcome host resistance. Here, we report a gapless genome fungus P. strain P131 using PacBio, Illumina high throughput chromatin conformation capture (Hi-C) sequencing data. This contained seven complete chromosomes (43,237,743 bp) circular...
Abstract Background Colletotrichum truncatum is a haploid, hemibiotrophic, ascomycete fungal pathogen that causes anthracnose disease on many economically important leguminous crops. This exploits sequential biotrophic- and necrotrophic- infection strategies to colonize the host. Transition from biotrophy destructive necrotrophic phase called biotrophy-necrotrophy switch critical in symptom development. C. likely secretes an arsenal of proteins are implicated maintaining compatible...
Abstract Ubiquitination is a vital protein post-translational modification (PTM) prevalent in eukaryotes. This regulates multiple cellular processes through degradation mediated by the 26S proteasome or affecting protein–protein interaction and localization. Magnaporthe oryzae causes rice blast disease, which one of most devastating crop diseases worldwide. In M. , ubiquitination plays important roles growth, pathogenicity, stress response effector-mediated plant-pathogen interaction. this...
Summary Maize is one of the most important crops for food, cattle feed and energy production. However, maize frequently attacked by various pathogens pests, which pose a significant threat to yield quality. Identification quantitative trait loci genes resistance pests will provide basis breeding in maize. Here, β‐glucosidase ZmBGLU17 was identified as gene against Pythium aphanidermatum , causal agents corn stalk rot, genome‐wide association analysis. Genetic analysis showed that both...
Maize leaf and sheath spot disease caused by Epicoccum sorghinum is an emerging of maize in China. To disentangle the molecular pathogenesis, we sequenced genome infection transcriptomes E. strain NJC07. The was on Oxford Nanopore GridION Illumina NovaSeq 6000, producing a near-complete gapless nuclear assembly 32.69 Mb at 285.20-fold depth, comprising 23 contigs (including 12 full-length chromosomes) with N50 contig number/length 6/1.66 Mb, complete mitochondrial 61.24 kb. contains 11,779...
The role of β-oxidation and the glyoxylate cycle in fungal pathogenesis is well documented. However, an ambiguity still remains over their interaction peroxisomes to facilitate pathogenicity virulence. In this report, we characterize a gene encoding alanine, aminotransferase 1 (AGT1) Magnaporthe oryzae, causative agent rice blast disease, demonstrate that AGT1 required for M. oryzae. Targeted deletion resulted failure penetration via appressoria; therefore, mutants lacking were unable induce...
Abstract Background Anthracnose of lentil, caused by the hemibiotrophic fungal pathogen Colletotrichum truncatum is a serious threat to lentil production in western Canada. employs bi-phasic infection strategy characterized initial symptomless biotrophic and subsequent destructive necrotrophic colonization its host. The transition from biotrophy necrotrophy (known as biotrophy-necrotrophy switch [BNS]) critical anthracnose development. Understanding plant responses during BNS key designing...
Sequencing of over 40 fungal and oomycete genomes has been completed. The next major challenge in modern fungal/oomycete biology is now to translate this plethora genome sequence information into biological functions. Reverse genetics emerged as a seminal tool for functional genomics investigations. Techniques utilized reverse like targeted gene disruption/replacement, silencing, insertional mutagenesis, targeting induced local lesions will contribute greatly the understanding function...
The hemibiotroph Colletotrichum lentis, causative agent of anthracnose on Lens culinaris (lentil) was recently described as a new species. During its interaction with the host plant, C. lentis likely secretes numerous effector proteins, including toxins to alter plant's innate immunity, thereby gaining access tissues for nutrition and reproduction.In silico analysis 2000 ESTs generated from lentis-infected lentil leaf identified 15 candidate effectors. In planta infection stage-specific gene...
Understanding the infection biology of fungi is key step in devising suitable control strategies for plant diseases. Recently, Arabidopsis-Colletotrichum higginsianum (causal agent anthracnose) system has emerged as a seminal paradigm deciphering underlying fungus-plant interactions. We describe here three staining methods coupled with confocal microscopy: trypan blue, aniline blue and dual blue-aniline fluorescence staining. Trypan were employed to scan structures hemibiotrophic fungus C....
Understanding the infection biology of fungi is key step in devising suitable control strategies for plant diseases. Recently, Arabidopsis-Colletotrichum higginsianum (causal agent anthracnose) system has emerged as a seminal paradigm deciphering underlying fungus-plant interactions. We describe here three staining methods coupled with confocal microscopy: trypan blue, aniline blue and dual blue-aniline fluorescence staining. Trypan were employed to scan structures hemibiotrophic fungus C....
Abstract Background The rice blast disease caused by Magnaporthe oryzae is a major constraint on world production. conidia produced this fungal pathogen are the main source of dissemination. morphology may be critical factor in spore dispersal and virulence M. field. Deletion conidial regulating gene encoding putative transcriptional regulator COM1 resulted aberrant shape, reduced conidiation attenuated virulence. Results In study, two-dimensional gel electrophoresis/matrix assisted laser...