A5101556445- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Malaria Research and Control
- Ion channel regulation and function
- Liver Disease Diagnosis and Treatment
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Drug-Induced Hepatotoxicity and Protection
- Virology and Viral Diseases
- Ion Transport and Channel Regulation
- Pain Mechanisms and Treatments
- Computational Drug Discovery Methods
- Peptidase Inhibition and Analysis
- Animal Disease Management and Epidemiology
- Mosquito-borne diseases and control
- Chemical Synthesis and Analysis
- Pharmacological Effects and Assays
- Eosinophilic Esophagitis
- Ion Channels and Receptors
- Hypothalamic control of reproductive hormones
- vaccines and immunoinformatics approaches
- Hepatitis C virus research
- Chemokine receptors and signaling
- Pediatric Hepatobiliary Diseases and Treatments
- Coccidia and coccidiosis research
Himalayan University
2020
Rai University
2020
Nishtar Medical College and Hospital
2019-2020
King Abdulaziz University
2015
AstraZeneca (United Kingdom)
2002-2012
AstraZeneca (Canada)
2001-2004
Centre for Research in Astrophysics of Québec
1998
University of California, Davis
1993-1997
Biotechnology Research Institute
1990-1992
McMaster University
1987-1990
Abstract Cannabinoids have been considered for some time as potent therapeutic agents in chronic pain management. Central and systemic administration of natural, synthetic endogenous cannabinoids produce antinociceptive antihyperalgesic effects both acute animal models. Although much the existing data suggest that analgesic are mediated via neuronal CB1 receptors, there is increasing evidence to support a role peripheral CB2 which expressed preferentially on immune cells. As yet, little...
A cDNA encoding a thyrotropin-releasing hormone (TRH) receptor expressed in the pituitary was previously cloned (De La Pena, P., Delgado, L. M., Del Camino, D., and Barros, F. (1992) Biochem. J. 284, 891–899; De Biol. Chem. 267, 25703–25708; Duthie, S. Taylor, P. L., Anderson, J., Cook, Eidne, K. A. (1993) Mol. Cell Endocrinol. 95, R11–R15). We now describe isolation of rat novel subtype TRH (termed TRHR2) displaying an overall homology 50% to receptor. Introduction TRHR2 HEK-293 cells...
Abstract To assess the validity of rodent models for investigating role delta opioid receptors (DOR) in analgesia, distribution DOR binding and mRNA were compared between primate spinal cord dorsal root ganglia (DRG), using receptor autoradiography situ hybridization, respectively. In mouse rat cord, [ 125 I]‐deltorphin‐labeled sites detected throughout gray matter. contrast, particularly human was mainly present laminae I–II, with little to no deeper layers. Accordingly, expressed by a...
Simian immunodeficiency virus (SIV) infection of macaques is a model for human (HIV) infection. We have previously reported the construction and characterization an SIV vector with deletion in nef gene (SIV(delta nef)) expressing gamma interferon (SIV(HyIFN)) (L. Giavedoni T. Yilma, J. Virol. 70:2247-2251, 1996). now show that rhesus vaccinated SIV(HyIFN) lower viral load than group similarly immunized SIV(delta nef). Viral loads remained low SIV(HyIFN)-vaccinated even though could not be...
Human formyl peptide‐receptor‐like‐1 (FPRL‐1) is a promiscuous G protein‐coupled receptor (GPCR), and belongs to chemoattractant family protein. This has been reported interact with various host‐derived peptides lipids involved in inflammatory responses. We described here, novel role for FPRL‐1 as high‐affinity β ‐chemokine an N‐terminally truncated form of the CK 8 (CCL23/MPIF‐1) splice variant 8‐1 (22–137 aa). RT‐PCR analysis mRNA derived from human tissues cells revealed predominant...
Rinderpest is a highly contagious viral disease of ruminants and has greater than 95% morbidity mortality. The etiological agent, rinderpest virus (RPV), member the family Paramyxoviridae genus Morbillivirus. Immune responses to both hemagglutinin (H) fusion (F) antigens morbilliviruses play an important role in prevention infection, only attenuated live vaccines have been shown provide protective immunity against group. lack protection with inactivated attributed denaturation F glycoprotein...
Background & aims Upper gastrointestinal bleeding (UGIB) is a common medical emergency that results in high patient morbidity and mortality. There are numerous causes of UGIB. The aim our study was to evaluate the endoscopic findings patients UGIB this part world. Methods This retrospective conducted at Department Gastroenterology Nishtar Medical University Hospital Multan from June 2018 March 2019. Record all undergoing esophagogastroduodenoscopy (EGD) for evaluation reviewed. Data entered...
The objectives of this study are 1) to examine the potential [3H]saxitoxin binding as a marker for neuronal membranes in canine small intestinal muscle membrane preparations, 2) develop synaptosomal preparation from deep muscular plexus, and 3) partially characterize fraction. A purified fraction, relatively low smooth plasma enzyme 5'-nucleotidase but enriched (2,592 fmol/mg), was obtained on sucrose density gradient. Vasoactive peptide immunoreactivity also highest (51.82 pmol/mg protein)...
Postsynaptic α-adrenoceptor subtypes were studied using [ 3 H]prazosin and H]rauwolscine binding to plasmalemma-enriched microsomal fractions isolated from dog saphenous veins mesenteric veins. Both radioligands showed saturable consistent with the presence of a single homogeneous site in each case, based on Scatchard analysis. The K d values H]rauwolscine, calculated kinetic studies similar those equilibrium data both venous muscle membranes. membranes vein contained about fourfold higher...
The distribution of plasma membrane markers, the sodium pump [evaluated as ouabain-sensitive, potassium-stimulated p-nitrophenyl phosphatase (K+-pNPPase)], [3H]saxitoxin binding, and 5'-AMPase, was studied in subcellular fractions prepared from homogenates longitudinal smooth muscle/myenteric plexus dog ileum. K+-pNPPase activity [3H]-saxitoxin binding were found to be predominantly associated with synaptosomal fraction indicated by high level these activities crude copurification but not...
TRPV1 was originally characterized as an integrator of various noxious stimuli such capsaicin, heat, and protons. TRPV1-null mice exhibit a deficiency in sensing heat stimuli, suggesting that is one the main sensors on nociceptive primary afferent neurons candidate target for hypersensitivity chronic pain. Several different potent selective antagonists have been developed by more than 50 companies since characterization receptor 1997. A consequence this competitive interest crowding...
We established the content in neuropeptide-metabolizing peptidases present highly purified plasma membranes prepared from circular and longitudinal muscles of dog ileum. Activities were measured by use fluorigenic substrates identities enzymes confirmed specific peptidase inhibitors. Endopeptidase 24.11, angiotensin-converting enzyme, post-proline dipeptidyl aminopeptidase aminopeptidases found both membrane preparations. Proline endopeptidase was only detected smooth muscle while...
The influences of adrenergic nerves on pyloric motor function and the locus types receptors involved were examined. Using glyoxylic acid fluorescence a dense innervation inner muscle ring was demonstrated. Pyloric activity monitored while close i.a. injections selective alpha-1 adrenoceptor agonist, phenylephrine, or alpha-2 B-HT-920, given. Neither agonist affected basal activity, but both inhibited when it stimulated by duodenal field stimulation intraduodenal infusion. actions each...