A5003583788- Microbial Inactivation Methods
- Microfluidic and Bio-sensing Technologies
- Magnetic and Electromagnetic Effects
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Electromagnetic Fields and Biological Effects
- Digital Holography and Microscopy
- Nicotinic Acetylcholine Receptors Study
- Radiation Effects and Dosimetry
- Mycotoxins in Agriculture and Food
- Microbial Natural Products and Biosynthesis
- Transgenic Plants and Applications
- Lipid Membrane Structure and Behavior
- Electrical and Bioimpedance Tomography
- Fungal and yeast genetics research
- Spectroscopy and Quantum Chemical Studies
- Cell Image Analysis Techniques
- Neural dynamics and brain function
- Microbial Metabolism and Applications
- Force Microscopy Techniques and Applications
United States Air Force Research Laboratory
2016-2023
59th Medical Wing
2020
Northern Illinois University
2014-2015
Short infrared laser pulses (SILP) have many physiological effects on cells, including the ability to stimulate action potentials (APs) in neurons. Here, we show that SILPs can also reversibly block APs. Reversible AP hippocampal neurons was observed following SILP (0.26 0.96 J/cm2; 1.37 5.01 ms; 1869 nm) with persisting for more than 1 s exposures greater 0.69 J/cm2. sustained 30 pulsed at 7 Hz. Full recovery of neuronal activity 5 post exposure. These results indicate be used noncontact,...
Cell suspensions of Escherichia coli and Lactobacillus acidophilus were exposed to 600-ns pulsed electric fields (nsPEFs) at varying amplitudes (Low-13.5, Mid-18.5 or High-23.5 kV cm-1) pulse numbers (0 (sham), 1, 5, 10, 100 1000) a 1 hertz (Hz) repetition rate. The induced temperature rise generated these exposure parameters, hereafter termed thermal gradient, was measured applied independently cell in order differentiate inactivation triggered by field (E-field) from heating. Treated...
Exposure of cells to very short (<1 µs) electric pulses in the megavolt/meter range have been shown cause a multitude effects, both physical and molecular nature. Physically, nanosecond electrical (nsEP) can disruption plasma membrane, cellular swelling, shrinking blebbing. Molecularly, nsEP activate signaling pathways, produce oxidative stress, stimulate hormone secretion induce apoptotic necrotic death. We hypothesize that studying genetic response primary human dermal fibroblasts exposed...
Cell-circuit models have suggested that nanosecond pulsed electric fields (nsPEFs) can disrupt intracellular membranes including endoplasmic reticulum (ER), mitochondria, and/or nucleus thereby inducing intrinsic apoptotic pathways. Therefore, we hypothesized the unfolded protein response (UPR) would be activated, due to fluctuations of ionic concentrations, upon poration ER membrane. Quantitative real-time polymerase chain reaction was utilized measure changes in messenger RNA (mRNA)...
Quantitative measurements of water content within a single cell are notoriously difficult. In this work, we introduce single-shot optical method for tracking the intracellular content, by mass and volume, at video rate. We utilize quantitative phase imaging priori knowledge spherical cellular geometry, leveraging two-component mixture model to compute content. apply technique study CHO-K1 cells responding pulsed electric field, which induces membrane permeabilization rapid influx or efflux...
Recently, infrared light has been shown to both stimulate and inhibit excitatory cells. However, studies of for cell inhibition have constrained by the use invasive cumbersome electrodes excitation action potential recording. Here, we present an all optical experimental design neuronal excitation, inhibition, detection. Primary rat neurons were transfected with plasmids containing sensitive ion channel CheRiff. CheRiff a peak around 450 nm, allowing pulsed blue light. Additionally, primary...
Short infrared laser pulses have many physiological effects on cells including the ability to stimulate action potentials in neurons. Here we show that short can also reversibly block potentials. Primary rat hippocampal neurons were transfected with Optopatch2 plasmid, which contains both a blue-light activated channel rhodopsin (CheRiff) and red-light fluorescent membrane voltage reporter (QuasAr2). This optogenetic platform allows robust stimulation recording of potential activity...
Water is the most basic requirement of life, acting as a medium exchange, transport, homeostasis and diffusion. We present technique which leverages quantitative phase imaging (QPI) two-component mixture model to quantify, in absolute terms, intracellular water fraction (by mass volume) well volume femtoliters. demonstrate our by applying single microsecond-duration electric pulse, observe influx physiological buffer, efflux hyperosmotic no movement across membrane synthetic vesicles. Dose...
Cytometry is a ubiquitous tool in biological sciences, allowing rapid determination of cellular health. We present single-shot microscope capable simultaneously determining fifteen biophysical parameters, including the water content (by mass and volume), longitudinal modulus, refractive index, density, other parameters.
A great deal of research has been focused on the study dynamics single cells exposed to short duration, (<1µs) high peak power (~1 MV/m) transient electric fields. Currently, most this is limited use traditional fluorescence-based microscopy techniques, which introduce exogenous agents culture and are only sensitive a specific molecular target depending dye used. Quantitative phase imaging (QPI) coherent modality uses optical pathlength (OPL) as label-free contrast mechanism, proven highly...
Our group recently published direct observation of membrane charging in FluoVolt labeled CHO-K1 cells by nanosecond electrical pulses using a streak camera. Using this technique, called Streak Camera Microscopy (SCM), we imaged the dynamics giant unilamellar vesicles (GUVs) during AC exposures up to 6 MHz and compared these results existing capacitive circuit models membranes. This work shows further application for evaluation high speed biological events.