A5032878739- Renal and related cancers
- Epigenetics and DNA Methylation
- Genetic and Kidney Cyst Diseases
- Molecular Biology Techniques and Applications
- Reproductive Biology and Fertility
- Sperm and Testicular Function
Hebrew University of Jerusalem
2021-2023
A hallmark of meiosis is chromosomal pairing, which requires telomere tethering and rotation on the nuclear envelope through microtubules, driving chromosome homology searches. Telomere pulling toward centrosome forms "zygotene bouquet." Here, we identified cilium" in oocytes. This cilium provides a cable system for bouquet machinery extends throughout germline cyst. Using zebrafish mutants live manipulations, demonstrate that anchors to counterbalance pulling. The essential synaptonemal...
Oocytes develop in the germline cyst, a cellular organization which germ cells are tightly interconnected and surrounded by somatic cells. The cyst produces oocytes for follicle formation is hub essential processes meiosis oocyte differentiation. However, of their contribution to production vertebrates remain unclear. Here, we provide tools three-dimensional functional vivo analyses zebrafish ovary. We describe use serial block-face scanning electron microscopy (SBF-SEM) resolve architecture...
Abstract The hallmark of meiosis is chromosomal pairing and synapsis via synaptonemal complexes, but also depends on cytoplasmic counterparts that tether rotate telomeres the nuclear envelope. Telomeres slide perinuclear microtubules, shuffling chromosomes mechanically driving their homology searches. Pull towards centrosome drives formation “zygotene bouquet”. These telomere dynamics are essential for fertility, bouquet, discovered in 1900, universally conserved. Nevertheless, how bouquet...