A5010697558- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Rabies epidemiology and control
- Mosquito-borne diseases and control
- Toxoplasma gondii Research Studies
- Bird parasitology and diseases
- T-cell and Retrovirus Studies
- Insect and Pesticide Research
- Parasitic Infections and Diagnostics
- Yersinia bacterium, plague, ectoparasites research
- Herpesvirus Infections and Treatments
- Animal Disease Management and Epidemiology
- Insect symbiosis and bacterial influences
- Helminth infection and control
- Trypanosoma species research and implications
- Animal Behavior and Welfare Studies
- Animal Behavior and Reproduction
- Dermatological diseases and infestations
- Zoonotic diseases and public health
- Neurological diseases and metabolism
- Animal Genetics and Reproduction
- Coccidia and coccidiosis research
- Blood transfusion and management
- Research on Leishmaniasis Studies
Universidade Federal Rural do Rio de Janeiro
2015-2024
Departamento de Epidemiología
2016-2023
Universidade Federal do Rio de Janeiro
2021-2023
Universidade Federal Fluminense
2023
Universitario Francisco de Asís
2023
Universidade Federal dos Vales do Jequitinhonha e Mucuri
2021
Universidade Federal de Mato Grosso
2021
Universidade Federal de Minas Gerais
2021
Brazilian Agricultural Research Corporation
2021
Portuguese Military Academy
2017
Abstract This study aimed to perform a morphological, molecular and phylogenetic characterization of Borrelia theileri obtained from infected Rhipicephalus microplus in Brazil. Fifty engorged R. females cattle the municipality Seropédica, Rio de Janeiro, were analyzed for spirochetes by hemolymph smear. Macerated eggs positive ticks, as well blood bovine infested these glpQ, flaB hpt genes PCR. The PCR products purified sequenced analysis construction tree. Only 2% (1/50) ticks generated...
The aim of this study is to detect the presence tick-borne agents genera Rickettsia, Borrelia, Babesia, Ehrlichia and Anaplasma in ticks collected from native wild birds state Rio de Janeiro. Birds were captured observed carefully find ectoparasites. DNA detection hemoparasites was performed by means polymerase chain reaction (PCR). sequences obtained analyzed their homologies compared available isolates GenBank platform database. A total 33 20 different species, which 14 parasitized...
Abstract Parasites of the genus Trypanosoma are unicellular flagellated microorganisms Trypanosomatidae . This study describes an isolate naturally infecting Rhipicephalus microplus ticks, characterized through molecular, morphological and biological analysis. Trypanosome cultures, designated strain P1RJ, were obtained by isolation from R. haemolymph in cultures tick cell line IDE8. After isolation, P1RJ grew well axenically L15B medium at temperatures 30, 32 34 °C. The new trypanosome...
The present study aimed to characterize the importance of Anaplasma marginale, Babesia bovis and bigemina in genesis cattle tick fever (CTF) among dairy calves northwest Minas Gerais, Brazil. Blood samples from 300 were collected, followed by DNA extraction nested PCR using oligonucleotide primers amplify fragments semi-nested for msp5 gene (A. marginale), sbp-4 (B. bovis) rap-1a bigemina) Among examined calves, prevalence A. marginale was 55.6% (n=167/300), B. 4.0% (n=12/300) 15.3%...
Abstract Background Currently, various zoonotic diseases are classified as emerging or reemerging. Because equids have a direct relationship with vectors, they possibly more frequently exposed to agents than humans. The undeniable importance of such human granulocytic anaplasmosis, spotted fever, and leishmaniasis for both public animal health, well the possibility acting sources, reservoirs, even sentinels these pathogens, justifies detection their frequency factors associated infection in...
Anaplasmataceae bacteria are emerging infectious agents transmitted by ticks. The aim of this study was to identify the molecular diversity bacterial family in ticks and hosts, both domestic wild, as well blood meal sources free-living northeastern Paraguay. were identified using PCR-HRM, a method optimized for purpose, while identification their performed conventional PCR. All amplified products subsequently sequenced. detected hosts included
Abstract Parasites of the genus Trypanosoma are microorganisms that display wide morphological, biological and genetic variability. Here we present first description an isolate naturally infecting tick Amblyomma brasiliense . The ticks were collected from a specimen Tayassu pecari (Queixada, white-lipped peccary) Itatiaia National Park, Itatiaia, Rio de Janeiro, Brazil. was characterized by molecular, morphometric analyses. A culture isolated crushed nymphal adult ticks, propagated in cell...
Abstract This study evaluated the influence of initial weight, feeding period and temperature on weight gain biological parameters non-parasitic phase partially engorged Rhipicephalus sanguineus females that were artificially fed using plastic tips as devices. The device did not alter oviposition or any other evaluated. Furthermore, it was observed group affect biology ticks. has great potential for development studies bioagent transmission because provides higher intake blood by ixodid
ABSTRACT The present paper is the first to perform this evaluation in dogs from cities of Natividade, Porciuncula and Varre-Sai. aim study search for Spotted Fever Group Rickettsia canine sera using indirect immunofluorescence assay identify probable causative agent reactions animals. Of 253 sampled canines, 67.59% (171/253) were seroreactive rickettsii 11.07% (28/253) parkeri, both dilution 1:64. Titration tested against R. antigens reached 1:131.072 and, 1:4.096. We conclude that are...
Rickettsia rickettsii is the causative agent of Brazilian spotted fever (BSF), for which humans and dogs are both susceptible. Dogs sentinels in serological surveys, however, canine disease rarely reported. Therefore, we aimed to evaluate natural infection by group (SFG) spp. ticks collected from domiciles close forest fragments, featuring domestic-wildlife interface areas. Samples 115 135 ixodids were assessed polymerase chain reactions (PCR) targeting gltA gene ompA SFG rickettsial...
Abstract Spotted fever group rickettsioses are emerging diseases. In some of these diseases, domestic dogs act as sentinels. Canine serological studies have demonstrated that rickettsial dispersion is concentrated in rural areas, seroprevalence being higher where human endemic. Rio de Janeiro, the Atlantic forest vegetation has been devastated by urbanization. this context, we aimed to detect Rickettsia spp. urban areas West Zone Janeiro. Sera from 130 were tested Indirect Immunofluorescence...
Abstract Background Canine monocytic ehrlichiosis (CME) is caused by the tick-borne pathogen Ehrlichia canis , an obligate intracellular Gram-negative bacterium of family Anaplasmataceae with tropism for canine monocytes and macrophages. The trp36 gene, which encodes major immunoreactive protein TRP36 in E. has been successfully used to characterize genetic diversity this different regions world. Based on sequence analysis, four genogroups, United States (US), Taiwan (TWN), Brazil (BR) Costa...
ABSTRACT: The study aimed to evaluate and compare the clinical, laboratory pathological aspects of buffalo bovine experimentally infected with AmRio 2 strain Anaplasma marginale. Four Murrah buffaloes four crossbred cattle were used in experiment, which two animals each species splenectomized. Strain A. marginale was inoculated all experimental animals. Clinical exams, Packed Cell Volume (PCV), blood counts, smears, rickettsemia, necropsy histopathology performed cases. Semi-Nested-PCR...
Resumo: Para padronização de uma técnica manual para a obtenção plasma rico em plaquetas (PRP) autólogo bovinos com custo reduzido (método manual) e boa qualidade (capacidade concentrar plaquetas, alta concentração fatores crescimento contaminação reduzida leucócitos eritrócitos), que poderá ser utilizado como um agente modulador da resposta imune vacas diferentes enfermidades, 450 ml sangue total nove clinicamente saudáveis perfil hematológico normal foi coletado bolsas CPDA-1 processado...
The objective of this study was to evaluate the biological parameters Rhipicephalus microplus engorged females exposed arthropod growth regulator fluazuron. A commercial formulation based on fluazuron (Acatak®, Novartis) administered topically stabled cattle artificially infested with R. larvae. infestations occurred four alternate days (days -21, -17, -13 and -6) taking as day 0 (zero) treatment a single dose for treated group. Fifteen from each group were collected floor stalls at end...