A5028029518- Cholinesterase and Neurodegenerative Diseases
- Chemical synthesis and alkaloids
- Botanical Research and Chemistry
- Pharmacological Effects of Natural Compounds
- Allelopathy and phytotoxic interactions
- Microbial Natural Products and Biosynthesis
- Phytochemistry and Biological Activities
- Plant Toxicity and Pharmacological Properties
- Hormonal Regulation and Hypertension
- Plant biochemistry and biosynthesis
- X-ray Diffraction in Crystallography
- Fungal Biology and Applications
- Crystallization and Solubility Studies
- Flavonoids in Medical Research
- Plant-based Medicinal Research
- Phytochemicals and Antioxidant Activities
- Bee Products Chemical Analysis
- Adrenal Hormones and Disorders
- Phytochemical compounds biological activities
- Phytochemistry and Bioactive Compounds
- Crystallography and molecular interactions
- Natural product bioactivities and synthesis
- Marine Sponges and Natural Products
- Pain Mechanisms and Treatments
- Ginger and Zingiberaceae research
Nagoya City University
2015-2024
Tsumura Research Institute (Japan)
2019
Iwate Medical University
2018
China Medical University
2014-2016
Nihon University
2013-2016
University of Shizuoka
2012-2013
Washington State University
2013
Hokkaido University
2005-2011
Redox enzymes play a central role in generating structural complexity during natural product biosynthesis. In the postassembly tailoring steps, redox cascades can transform nascent chemical scaffolds into structurally complex final products. Chaetoglobosin A (1) is biosynthesized by hybrid polyketide synthase–nonribosomal peptide synthetase. It belongs to chaetoglobosin family of products, comprising many analogs having different degrees oxidation introduced their We report here...
Abstract Fungal genome sequencing has revealed many genes coding for biosynthetic enzymes, including polyketide synthases and nonribosomal peptide synthetases. However, characterizing these enzymes identifying the compounds they synthesize remains a challenge, whether are expressed in their original hosts or more tractable heterologous hosts, such as yeast. Here, we developed streamlined method isolating from fungal sources producing bioactive molecules an engineered Saccharomyces cerevisiae...
Postgenomic analysis revealed that many microorganisms carry numerous secondary metabolite biosynthetic genes on their genome. However, activities of those putative are not clearly reflected in the metabolic profile microorganisms, especially fungi. A recent genome mining effort is promising discovering new natural products. fungi and other organisms amenable to molecular genetics manipulations, making study difficult. Here we report successful engineering Chaetomium globosum, a known...
The p53 tumor suppressor plays critical roles in cell cycle regulation and apoptotic death response to various cellular stresses, thereby preventing cancer development. Therefore, the activation of through small molecules is an attractive therapeutic strategy for treatment cancers retaining wild-type p53. We used a library 700 Myanmar wild plant extracts identify that induce transcriptional activity. A cell-based screening method with p53-responsive luciferase-reporter assay system revealed...
Fungal genomes carry many gene clusters seemingly capable of natural product biosynthesis, yet most remain silent. This places a major constraint on the conventional approach cloning these genes in more amenable heterologous host for biosynthesis. One way to overcome this difficulty is activate silent within context target fungus. Here, we successfully activated polyketide biosynthetic cluster Aspergillus oryzae by overexpressing transcriptional regulator found from plasmid. strategy allowed...
Abstract Pseudoaldosteronism is a common adverse effect associated with traditional Japanese Kampo medicines. The pathogenesis mainly caused by 3-monoglucuronyl glycyrrhetinic acid (3MGA), one of the metabolites glycyrrhizin (GL) contained in licorice. We developed an anti-3MGA monoclonal antibody (MAb) and ELISA system to easily detect 3MGA plasma urine patients. However, we found that some GL cross-reacted this MAb. Mrp2-deficient Eisai Hyperbilirubinemia rats (EHBRs) were administered...
Licorice-induced pseudoaldosteronism is a common adverse effect in traditional Japanese Kampo medicine, and 3-monoglucuronyl glycyrrhetinic acid (3MGA) was considered as causative agent of it. Previously, we found 22α-hydroxy-18β-glycyrrhetyl-3-O-sulfate-30-glucuronide (1), one the metabolites glycyrrhizin (GL) urine Eisai hyperbilirubinuria rats (EHBRs) treated with (GA), suggested that it also possible pseudoaldosteronism. The discovery 1 there might be other GA causal candidates. In this...
ABSTRACT 4-Hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF) is a key aroma compound in Fragaria × ananassa (strawberry). A considerable amount of HDMF converted into β-D-glucoside and accumulated mature strawberry fruits. Here we isolated novel UDP-glucose: glucosyltransferase, UGT85K16 from ananassa. preferentially glucosylated the hydroxyl group its structural analogs. Although also catalyzed glucosylation vanillin, affinity efficiency toward was higher. The expression mRNA correlated with...