A5014520949- Cellular transport and secretion
- Calcium signaling and nucleotide metabolism
- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Toxin Mechanisms and Immunotoxins
- Ion channel regulation and function
- Lipid Membrane Structure and Behavior
- Adenosine and Purinergic Signaling
- Plant-Microbe Interactions and Immunity
- Adipose Tissue and Metabolism
- PARP inhibition in cancer therapy
- Glycosylation and Glycoproteins Research
- Receptor Mechanisms and Signaling
- RNA and protein synthesis mechanisms
- Phosphodiesterase function and regulation
- Endoplasmic Reticulum Stress and Disease
- Peptidase Inhibition and Analysis
- Pancreatic function and diabetes
- Muscle metabolism and nutrition
- Metabolism and Genetic Disorders
- Insect-Plant Interactions and Control
- Signaling Pathways in Disease
- Diet and metabolism studies
- Plant biochemistry and biosynthesis
- Plant responses to elevated CO2
National Center for Agricultural Utilization Research
2016-2025
Agricultural Research Service
2016-2025
United States Department of Agriculture
2014-2024
National Heart Lung and Blood Institute
2007-2020
National Institutes of Health
2008-2020
Agricultural Research Council of South Africa
2016
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria
2016
Peoria campus of the University of Illinois System
2016
Center for Medical, Agricultural and Veterinary Entomology
2011-2014
Virginia Tech
2011-2013
Oleic acid has a partition coefficient, upper phase/lower phase, of 1.9 (22 degrees C) in the liquid-liquid system described herein. Tri-, di-, and monoolein are found almost exclusively lower (organic) phase. can be quantitatively removed from mixtures triglyceride partial glycerides by means this under conditions resembling those lipase assay.
Using a new approach based on simultaneous measurements of net changes in glycerol and free fatty acids, the rates lipolysis acid esterification were estimated rat epididymal fat pads under various conditions vitro. Glycerol release, taken as measure rate lipolysis, was stimulated by epinephrine, norepinephrine, glucagon, adrenocorticotropic hormone (ACTH), thyroid-stimulating (TSH), growth (GH). When ACTH, or TSH, calculated also increased, although to lesser extent, course. GH, which...
About 30 years ago, Goldblatt (1, 2) and von Euler (3, 4) showed that human ovine seminal plasma contain an acidic lipid (prostaglandin) with vasodepressor activity smooth muscle-stimu- lating activity.The early studies have been sum- marized by (5, 6).In 1960 Berg- strom Sjdvall isolated from sheep vesicular glands a highly potent crystalline material, prostaglandin E1 (PGE1) (7).1 The structure of this compound (Figure 1) was established as 2 (6-carboxyhexyl) -3-(3-hydroxyocten-1-yl)...
Phytoalexins constitute a broad category of pathogen- and insect-inducible biochemicals that locally protect plant tissues. Because their agronomic significance, maize rice have been extensively investigated for terpenoid-based defenses, which include monoterpene sesquiterpene volatiles. Rice also produces complex array pathogen-inducible diterpenoid phytoalexins. Despite the demonstration fungal-induced ent -kaur-15-ene production in over 30 y ago, identity functionally analogous...
Insect-induced defenses occur in nearly all plants and are regulated by conserved signaling pathways. As the first described plant peptide signal, systemin regulates antiherbivore Solanaceae, but other families, peptides with analogous activity have remained elusive. In current study, we demonstrate that a member of maize (Zea mays) elicitor (Pep) family, ZmPep3, responses against herbivores. Consistent being expression ZmPROPEP3 precursor gene is rapidly induced Spodoptera exigua oral...
Trichothecenes are a family of terpenoid toxins produced by multiple genera fungi, including plant and insect pathogens. Some trichothecenes the fungus Fusarium among mycotoxins greatest concern to food feed safety because their toxicity frequent occurrence in cereal crops, trichothecene production contributes pathogenesis some species on plants. Collectively, fungi produce over 150 analogs: i.e., molecules that share same core structure but differ patterns substituents attached structure....
G protein-coupled receptor activation leads to the membrane recruitment and of kinases, which phosphorylate receptors lead their inactivation. We have identified a novel kinase-interacting protein, GIT1, that is GTPase-activating protein (GAP) for ADP ribosylation factor (ARF) family small GTP-binding proteins. Overexpression GIT1 reduced β 2 -adrenergic signaling increased phosphorylation, result from internalization resensitization. These cellular effects require its intact ARF GAP...
Abstract Homogenates of rat fat cells were separated into three fractions by centrifugation: P1 (sedimented at 10,000 x g for 7 min), P2 from the supernatant after 20 min 100,000 g), and S (the supernatant). All contained adenosine 3':5'-monophosphate (cAMP) phosphodiesterase activity. The fraction was enriched in higher Km (∼15 µm) low (∼0.2 activity, although both exhibited two apparent Michaelis constants cAMP. When assayed with 62 nm cAMP, specific activity 2 to 3 times that...
Phagocytic vesicles were isolated from rabbit alveolar macrophages and guinea pig polymorphonuclear leukocytes that had ingested emulsified paraffin oil. Phospholipids their fatty acids determined in whole cells the phagocytic vesicle pellet fractions separated them. The cholesterol-to-phospholipid ratios distinctly higher than those of respective or fractions. also phospholipid-to-protein did cells. phospholipids fraction contained relatively more sphingomyelin, lyso-(bis)phosphatidic acid,...
Polymorphonuclear leukocytes suspended in Krebs-Ringer phosphate medium ingest paraffin oil containing Oil Red O emulsified with a variety of substances. Spectrophotometric determination the cells after uningested particles have been removed by differential centrifugation provides quantitative measure phagocytosis. This system has used to investigate effects several drugs and hormones on initial rate phagocytosis approach question how surface particle influences its acceptability as...
Abstract Nonvolatile terpenoid phytoalexins occur throughout the plant kingdom, but until recently were not known constituents of chemical defense in maize (Zea mays). We describe a novel family ubiquitous sesquiterpenoid phytoalexins, termed zealexins, which discovered through characterization Fusarium graminearum-induced responses. Zealexins accumulate to levels greater than 800 μg g−1 fresh weight F. graminearum-infected tissue. Their production is also elicited by wide variety fungi,...
Abstract Isolated fat cells were incubated in Krebs-Ringer phosphate medium. Within 1 min after the addition of 1.1 µm epinephrine, cyclic AMP content was elevated. It continued to rise, reaching a peak 4 7 min, and fell thereafter, being only slightly above control levels 10 15 min. A maximal rate glycerol production established within 2 well before concentration, maintained unchanged while level rise then fell. Both magnitude time course, effect adrenocorticotropin (ACTH), 0.2 unit per ml,...