A5071899592- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Genetics, Aging, and Longevity in Model Organisms
- Diabetes and associated disorders
- Parkinson's Disease Mechanisms and Treatments
- Circadian rhythm and melatonin
- Genetic Associations and Epidemiology
- Endoplasmic Reticulum Stress and Disease
- Phosphodiesterase function and regulation
- Liver Disease Diagnosis and Treatment
- Neuroendocrine regulation and behavior
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Case Western Reserve University
2008-2016
Mutation of leucine-rich repeat kinase 2 (LRRK2) is the leading genetic cause Parkinson's Disease (PD), manifested as age-dependent dopaminergic neurodegeneration, but underlying molecular mechanisms remain unclear. Multiple roles LRRK2 may contribute to neurodegeneration. Endoplasmic reticulum (ER) stress has also been linked PD pathogenesis, its interactive mechanism with factors largely unknown. Here, we used C. elegans, human neuroblastoma cells and murine cortical neurons determine role...
Activation of G-protein-coupled receptors (GPCRs) initiates signal transduction cascades that affect many physiological responses. The worm Caenorhabditis elegans expresses >1000 these along with their cognate heterotrimeric G proteins. Here, we report properties 9-cis-retinal regenerated bovine opsin [(b)isoRho] and human melanopsin [(h)Mo], two light-activated, heterologously expressed GPCRs in the nervous system C. various genetically engineered alterations. Profound transient...
New strategies for expression, purification, functional characterization, and structural determination of membrane-spanning G-protein-coupled receptors (GPCRs) are constantly being developed because their importance to human health. Here, we report a Caenorhabditis elegans heterologous expression system able produce milligram amounts native engineered GPCRs. Both bovine opsin [(b)opsin] adenosine A2A subtype receptor [(h)A2AR] expressed in neurons or muscles C. were localized cell membranes....