Background: The use of face masks was a significant part the WHO COVID-19 preventive protocols. While their usage has been effective, lack adherence by individuals associated with discomfort and adverse side effects. This might facilitate unnecessary exposure to SARS-CoV-2 virus, thereby increasing incidence COVID-19. study assessed effects prolonged mask-wearing offers recommendations for present future pandemics. Methods: Adverse were evaluated from November 2021 February 2022 structured...

ABSTRACT Objectives The defense mechanisms in bacterial pathogens protect them from host immune systems, bacteriophage infections and stringent environmental conditions. This study explores the defense-systems multidrug resistant Klebsiella pneumoniae isolated Ghanaian hospital ICUs focusing on CRISPR-cas, restriction-modification toxin-antitoxin systems (TAs). Method Genomic DNA of K. (NS2) clinical (PS4) strains were subjected to whole genome sequencing using Illumina assembled with SPAdes...

In the absence of potent antimicrobial agents, it is estimated that bacterial infections could cause millions deaths. The emergence COVID-19, its complex pathophysiology and high propensity patients to coinfections has resulted in therapeutic regimes use a cocktail antibiotics for disease management. Suboptimal stewardship this era slow pace drug discovery result large-scale resistance, narrowing future therapeutics. Thus, judicious current antimicrobials imperative keep up with existing...

Acute gastroenteritis (AGE) is a disease of global public health importance. Recent studies show that children with AGE have an altered gut microbiota relative to non-AGE controls. Yet, how the differs in Ghanaian and without remains unclear. Here, we explore 16S rRNA gene-based faecal profiles five years age younger, comprising 57 cases 50 healthy We found were associated lower microbial diversity sequence The was enriched for disease-associated bacterial genera, including Enterococcus,...

Uterine fibroid (UF) is the most prevalent benign tumour that affects millions of women globally, with a high incidence 70% amongst reproductive age. UF has been associated various complications, such as recurrent surgeries, infertility, anemia and pregnancy loss. Notably, African descent often experience more severe symptoms complications. Although hormones, growth factors, genetic alterations are widely UF, precise mechanism underlying its pathogenesis not fully understood. Recent evidence...

Nose mask is a fomite and its usage was mandatory during the COVID-19 outbreak. Its effectiveness has been established; however, as fomite, it can harbor opportunistic respiratory pathogens such Streptococcus spp., which could complicate infections. This study profiled species resident on nose masks obtained from consenting participants. Phenotypic assays 16S rRNA sequencing were used to identify spp. interior exterior of 100 analyzed. Antibiotic resistance (AMR) determined with disc...

<ns3:p><ns3:bold>Background:</ns3:bold> Use of nose masks was an effective and significant part the World Health Organization (WHO) coronavirus disease (COVID-19) preventive protocols; however, unhygienic practices by user could lead to microbial contamination masks. This study profiled bacteria fungi resident on established practices; which not prioritized during COVID-19 pandemic.</ns3:p><ns3:p> <ns3:bold>Methods:</ns3:bold> a cross-sectional exploratory study, questionnaires toward mask...

<ns4:p><ns4:bold>Background:</ns4:bold>The study was conducted to determine the bacterial composition and antibiotic susceptibility profiles of a research institute at University Ghana where workers students spend about 70-85% their lives in indoor immediate-outdoor environments. This is imperative as one-third recognized infectious diseases are transmitted through airborne-route. Furthermore, increasing rate antimicrobial resistance associated with such environments poses serious public...

The public health risk associated with the autopsy unit is high and information on indoor microbial air contamination limited. Airborne pathogenic fungi thrive in moist environments similar to room conditions. They spread through spores, cause infections immunocompromised patients, exhibit resistance antimicrobials, making them a threat. This study profiled circulating of referral Accra, Ghana. different sections were sampled using standard open plate technique. Fungal isolates obtained...

Abstract Background Buruli ulcer (BU) disease, caused by Mycobacterium ulcerans (MU), and characterized necrotic ulcers is still a health problem in Africa Australia. The genome of the bacterium has several pseudogenes due to recent evolutionary events environmental pressures. Pseudogenes are genetic elements regarded as nonessential bacteria, however, they less studied limited available tools provide understanding their evolution roles MU pathogenicity. Results This study developed...

Shigella and Salmonella have been implicated in neonatal sepsis, food poisoning, bacteremia. Their increasing resistance to antibiotics has undermined treatment options, constituting a public health crisis. Also, the scarcity of new development pipeline compounds multidrug-resistance (MDR). Hence, there is need potentiate mitigate resistance. This study leveraged riboflavin re-sensitize fluoroquinolones carbapenems against MDR spp. Disk diffusion, microbroth-dilution, chequerboard, time-kill...

<ns3:p><ns3:bold>Background</ns3:bold>: Antibacterial agents, including disinfectants and antiseptics are commonly used to reduce bacterial loads. As they have a broad-spectrum of activity against bacteria, function either as bactericidal or bacteriostatic agents. While antimicrobial resistance is increasing, still relevant antibacterial </ns3:p><ns3:p> <ns3:bold>Methods</ns3:bold>: This study investigated the <ns3:italic>in vitro</ns3:italic> inhibitory effects disinfectants. Using standard...

<ns4:p><ns4:bold>Background:</ns4:bold>The study was conducted to determine the bacterial composition and antibiotic susceptibility profiles of a research institute at University Ghana where workers students spend about 70-85% their lives in indoor immediate-outdoor environments. This is imperative as one-third recognized infectious diseases are transmitted through airborne-route. Furthermore, increasing rate antimicrobial resistance associated with such environments poses serious public...

Antimicrobial resistance (AMR) remains a global health challenge, as bacteria display increasing to last-resort antibiotics such carbapenems. Enterobacter cloacae are evolving and developing high level of With AMR, availability for treatment dwindles, hence need complement enhance activity or reduce the resistance. This study explored use calcium ions in attenuating bacterial carbapenems.E. strains isolated from hospital fomites air were subjected antimicrobial susceptibility testing with...

Sporulating bacteria such as Bacillus spp. have contributed to severity of opportunistic hospital acquired infections, including postoperative wounds and respiratory tract infections. This study determines the expression profiles sporulation markers in multidrug-resistant isolated from Ghanaian environments. Antimicrobial resistance (AMR) were determined with disk diffusion broth microdilution. Primer-specific polymerase chain reaction (PCR) amplification was used profile markers,...

Urinary tract infections (UTIs) are frequent hospital-acquired infection, with Escherichia coli and Proteus mirabilis accounting for 90% of complicated UTIs. Emergence multidrug-resistant (MDR) bacteria have the treatment P. related UTIs has been associated production urinary stones long-term in patients catheters. other uropathogens constitute a largely unexplored pathogen group. The is resistant to most antibiotics as result its impermeable outer membrane (OM). β-barrel assemble machinery...

<ns3:p><ns3:bold>Background</ns3:bold>: Antibacterial agents, including disinfectants and antiseptics are commonly used to reduce bacterial loads. As they have a broad-spectrum of activity against bacteria, function either as bactericidal or bacteriostatic. While antimicrobial resistance is increasing, still relevant antibacterial agents. </ns3:p><ns3:p> <ns3:bold>Methods</ns3:bold>: This study investigated the <ns3:italic>in vitro</ns3:italic> inhibitory effects disinfectants. Using...