Abstract Experimental elucidation of the metabolic load placed on bacteria by expression foreign protein is presented. The host/vector system Escherichia coli RR1/pBR329 (amp r , cam and let ). Plasmid content results, which indicate that plasmid copy number monotonically increases with decreasing growth rate, are consistent literature ColE1‐like plasmids. More significantly, we have experimentally quantified reduction in rate brought about chloramphenicol‐acetyl‐transferase (CAT)...

Sedimentation, wherein particles fall under the action of gravity through a fluid in which they are suspended, is commonly used chemical and petroleum industries as way separating from fluid, well with different settling speeds each other. Examples such separations include dewatering coal slurries, clarifi­ cation waste water, processing drilling mining fluids containing rock mineral various sizes. The separation by sedimentation also basis some laboratory techniques for determining...

The dynamic deformation of a solid elastic sphere which is immersed in viscous fluid and close motion toward another or plane surface presented. deformed shape the surfaces pressure profile layer separating these are determined simultaneously via asymptotic numerical techniques. This research provides first steps establishing rational criteria for predicting whether particle will stick rebound subsequent to impact during filtration coagulation when forces important.

Prebreakdown phenomena in water were investigated for point-plane geometries using high-voltage pulses. Spot discharges, filamentary magenta streamers, isolated microdischarges, and microbubbles observed photographed. Emission spectra obtained a prism spectrograph. Maximum streamer lengths determined as function of applied voltage, pulsewidth (decay constant), conductivity. The bubbling gas through the underwater discharge resulted disintegration bubbles, also caused gas-phase discharges to...

Dr. Ralph Wynn has edited one of the finest books on uterus ever published. Distinguished scientists and medical men from here abroad have contributed harmoniously in expounding complex facets uterine phenomena. The interdisciplinary approach succeeds combining classic anatomic-physiologic concepts with recent findings. 13 chapters logical continuity great depth, some 1,500 references 1,000 subjects are cited. clinician is given basic scientific information, scientist can find suggestions...

A novel sequential ultraviolet (UV)-induced living graft polymerization method has been designed and investigated to modify polymeric materials. This consists of two steps. In the first step, a surface initiator is formed on substrate under UV irradiation in presence benzophenone (BP) solutions; second monomers are grafted by initiated photoinitiator. Hydrophobic porous polypropylene (PP) membranes were made hydrophilic with negatively charged grafting acrylic acid (AA). Experimental results...

The hydrodynamic force resisting the relative motion of two unequal drops moving along their line centers is determined for Stokes flow conditions. are assumed to be in near-contact and have sufficiently high interfacial tension that they remain spherical. squeeze narrow gap between analyzed using lubrication theory, within near axis symmetry a boundary integral technique. flows coupled through nonzero tangential stress velocity at interface. Depending on ratio drop viscosity continuous...

OBJECTIVES: This study examined the use of seclusion and mechanical restraint from 1990 to 2000 rate staff injuries patient assaults 1998 in a state hospital system. METHODS: Records patients older than 18 years who were civilly committed one nine hospitals Pennsylvania included analyses. Two databases used each hospitals: identified date, time, duration, justification for episode or other when was hospitalized demographic characteristics diagnosis patient. Rate duration calculated. Reports...

The scattering of alpha particles by ${\mathrm{C}}^{12}$ has been studied in the bombarding energy range 10 to 19 MeV. Many anomalies were observed excitation curves which correspond ${\mathrm{O}}^{16}$ compound system levels 14 21 Fifteen detailed angular distributions measured and data have analyzed with a smooth cutoff model was modified include appropriate resonant phase shifts. spins parities more prominent resonances assigned. Real well depths 100 MeV or required an optical-model...

The deformation of a viscous drop, driven by buoyancy towards solid surface or deformable interface, is analysed in the asymptotic limit small Bond number, for which becomes important only when drop close to interface. Lubrication theory used describe flow thin gap between and boundary-integral fluid phases on either side gap. evolution shape traced from relatively undeformed state until dimple formed long-time quasi-steady-state pattern established. A wide range suspending phase viscosity...

The performance of a new method using pulsed streamer corona for the removal SO <L xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</L> from humid air has been evaluated. produces energetic free electrons that excite, dissociate, and ionize gas molecules, forming radicals enhance gas-phase chemical reactions convert SO2 to acid mist and/or particulate aerosols. aerosols are then collected by conventional means, i.e., electrostatic precipitator or...

A pulse-energized electron reactor utilizing pulsed streamer corona has been developed for the combined removal of SO/sub 2/, NO/sub x/, and particles from effluent gases. In process, fast-rising narrow high voltage pulses are superimposed on a DC bias applied to nonuniform electric field geometry generate corona. The produces energetic free electrons, which dissociate gas molecules, forming radicals. These radicals cause chemical reactions that convert 2/ x/ into acid mists and/or solids,...

Experiments were performed in order to delineate the conditions under which small metal and plastic spheres rebound, as opposed stick, when dropped upon a smooth quartz surface overlaid with thin layer of viscous fluid. The parameters that varied include fluid thickness viscosity, ball size, density, elastic properties. minimum drop height allowed rebound out was determined. results are very good agreement recent elastohydrodynamic theory Davis et al. [J. Fluid Mech. 163, 479 (1986)]....

A new three-dimensional boundary-integral algorithm for deformable drops moving in a viscous medium at low Reynolds numbers is developed, which overcomes some familiar difficulties with calculations. The used to simulate different modes of interaction between or bubbles, primarily buoyancy-driven motion. present iterative method mean curvature calculation found be more robust and accurate than contour integration schemes. novel strategy based on combining biconjugate gradient simple...

Abstract Steady-state and transient models are reviewed for predicting flux decline crossflow microfiltration under conditions in which both external cake buildup internal membrane fouling contributing factors. Experimental work is not covered the scope of this review, although reference made to a few recent studies have compared experimental measurements with theory. The steady-state thickness permeate governed by concentration polarization layer adjacent rejected particles forms on...

The steepest descent methods of Bryson and Ho [1] Kelly [6] the conjugate gradient method Lasdon, Mitter, Waren [3] use control variables as independent in search procedure. inequality constraints are often handled via penalty functions which result poor convergence. Special difficulties encountered handling state variable singular arcs [1]. This paper shows that these arise due to exclusive An algorithm based on generalized reduced (GRG) Abadie Carpentier [5] [7] for nonlinear programming...

Yields of gamma rays from thick targets In, ${\mathrm{I}}^{127}$, ${\mathrm{Ta}}^{181}$, ${\mathrm{Re}}^{185,187}$, ${\mathrm{Ir}}^{191,193}$, ${\mathrm{Hg}}^{198,199,200,202}$, Th, and U were investigated under proton or alpha-particle bombardment in the energy range 2 to 4 Mev. By using theory for electric excitation, reduced matrix elements all electrically excited radiations computed compared with single-particle estimates. The results are interpreted terms strong-coupling collective...

The reactions ${\mathrm{Li}}^{6}(n, \ensuremath{\alpha}){\mathrm{H}}^{3}$, ${\mathrm{F}}^{19}(n, \ensuremath{\gamma}){\mathrm{F}}^{20}$, and ${\mathrm{I}}^{127}(n, \ensuremath{\gamma}){\mathrm{I}}^{128}$ were studied using scintillating crystals both as targets detectors. neutrons produced by the ${\mathrm{Li}}^{7}(p, n){\mathrm{Be}}^{7}$ ${\mathrm{T}}^{3}(p, n){\mathrm{He}}^{3}$ reactions. A redetermination of differential cross section was made in proton energy range 1.9 to 3.0 Mev at...