Leptin, a hormone secreted by peripheral adipose tissues, regulates the appetite in animals. Recently, evidence has shown that leptin also plays roles behavioral response addition to controlling appetite. In this study, we examined potential function of on non-appetite behaviors zebrafish model. By using genome editing tool Transcription activator-like effector nuclease (TALEN), successfully knocked out (lepa) gene deleting 4 bp within coding region create premature-translation stop....

In this study, we evaluated the acute (24, 48, 72, and 96 h) chronic (21 days) adverse effects induced by low doses (0.1, 0.5, 1, 1.5 mg/L) of zinc chloride (ZnCl₂) exposure in adult zebrafish using behavioral endpoints like three-dimensional (3D) locomotion, passive avoidance, aggression, circadian rhythm, predator avoidance tests. Also, brain tissues were dissected subjected to analysis multiple parameters related oxidative stress, antioxidant responses, superoxide dismutase (SOD),...

The measurement of multiple behavior endpoints in zebrafish can provide informative clues within neurobehavioral field. However, evaluations usually require complicated and costly instrumental settings. Here, we reported a versatile setting that applied ten acrylic tanks arranging into five vertical layers two horizontal columns to perform assays simultaneously, such as the novel tank diving test, mirror-biting social interaction, shoaling, predator escape assay. In total, behavioral...

Hepatocellular carcinoma (HCC) ranks as the fourth leading cause of cancer-related deaths worldwide. Sorafenib was only U.S. Food and Drug Administration (FDA) approved drug for treating advanced HCC until recently, so development new target therapy is urgently needed. In this study, we established a zebrafish screening platform compared therapeutic effects two multiple tyrosine kinase inhibitors, 419S1 420S1, with Sorafenib. All three compounds exhibited anti-angiogenesis abilities in...

Generally, the measurement of three-dimensional (3D) swimming behavior in zebrafish relies on commercial software or requires sophisticated scripts, and depends more than two cameras to capture video. Here, we establish a simple economic apparatus detect 3D locomotion zebrafish, which involves single camera system that records movement specially designed water tank with mirror tilted at 45 degrees. The recorded videos are analyzed using idTracker, while spatial positions calibrated by ImageJ...

With-no-lysine (K)-1 (WNK1) is the founding member of family four protein kinases with atypical placement catalytic lysine that play important roles in regulating epithelial ion transport. Gain-of-function mutations WNK1 and WNK4 cause a mendelian hypertension hyperkalemic disease. ubiquitously expressed essential for embryonic angiogenesis mice. Increasing evidence indicates role WNK tumorigenesis at least partly by stimulating tumor cell proliferation. Here, we show human hepatoma cells...

Zebrafish has an advantage of optical clarity within the body during its early developmental stage. Therefore, organs, including heart, can be observed and analyzed in a non-invasive manner. Previously, heart rate measurement was by complicated system, programming language, fluorescence transgenic zebrafish, or expensive setup that not feasible practical. In this study, we established simple dissecting microscope with Charge-coupled Device (CCD) to capture signal heartbeat, used ImageJ...

This study aimed to develop a simple and cost-effective method measure blood flow in zebrafish by using an image-based approach. Three days post fertilization (dpf) embryos were mounted with methylcellulose subjected video recording for tracking under inverted microscope equipped high-speed CCD camera. In addition, Hoffman lens was used enhance the cell contrast. The red movement tracked TrackMate plug-in ImageJ image processing program. Moreover, Stack Difference Time Series Analyzer detect...

Analysis of circadian rhythm behavior alteration in fish for toxicity assessment usually requires expensive commercial equipment and laborious complicated tweaking. Here, we reported a simple set-up that consists custom-made light box equipped with white 940 nm emitting diodes (LED) strips as sources the locomotion activities zebrafish or catfish were captured by using infrared-sensitive coupled charged device (CCD). The whole was housed temperature-controlled incubator to isolate external...

There is a growing concern for the potential toxicity of engineered nanomaterials that have made their way into virtually all novel applications in electronics, healthcare, cosmetics, technology, and engineering industries, particular, biomedical products. However, carbon 60 (C60) at behavioral level has not been properly evaluated. In this study, we used idTracker, multitracking algorithm to quantitatively assess induced by C60 nanoparticles (C60 NPs) adult zebrafish. We demonstrated...

Hepatocellular carcinoma ranks fourth in cancer-related mortality currently lacks effective therapeutics. Fucoidan is sulfated polysaccharide that mainly found brown seaweeds. In this study, we investigated the effects and mechanisms of low molecular weight fucoidan (i.e. oligo-fucoidan [OF]) preventing hepatocarcinogenesis.We used [HBx,src], [HBx,src,p53-/+ ], [CD36] transgenic zebrafish liver cancer model treated with OF, performed histopathological analysis. Transcriptomic pathways...

Aurora A kinase (AURKA) is an important regulator in mitotic progression and overexpressed frequently human cancers, including hepatocellular carcinoma (HCC). Many AURKA mutations were identified cancer patients. Overexpressing wild-type Aurka developed a low incidence of hepatic tumors after long latency mice. However, none the mutant animal models have ever been described. The mechanism AURKA-mediated hepatocarcinogenesis still unclear. novel mutation with a.a.352 Valine to Isoleucine...

Abstract Background Congenital myopathy (CM) is a group of clinically and genetically heterogeneous muscle disorders, characterized by weakness hypotonia from birth. Currently, no definite treatment exists for CM. A de novo mutation in Tropomyosin 3- TPM3 (E151G) was identified boy diagnosed with CM, previously (E151A) reported to cause However, the role CM unknown. Methods Histopathological, swimming behavior, endurance were monitored wild-type mutant transgenic fish, modelling Gene...

Safety is one of the most important and critical issues in drug development. Many drugs were abandoned clinical trials retracted from market because unknown side effects. Cardiotoxicity common reasons for retraction due to its potential effects, i.e., inducing either tachycardia, bradycardia or arrhythmia. The zebrafish model could be used screen libraries with cardiotoxicity a high-throughput manner. In addition, fundamental principles replacement, reduction, refinement laboratory animal...

Previous methods to measure protozoan numbers mostly rely on manual counting, which suffers from high variation and poor efficiency. Although advanced counting devices are available, the specialized usually expensive machinery precludes their prevalent utilization in regular laboratory routine. In this study, we established ImageJ-based workflow quantify ciliate a high-throughput manner. We conducted Tetrahymena number measurement using five different methods: particle analyzer method (PAM),...

In this study, the complete mitogenome sequence of sea slug, Chromodoris annae (Mollusca: Chromodorididae), has been decoded for first time by genome skimming method. The overall base composition C. is 30.6% A, 14.5% C, 17.8% G, and 37.2% T, GC content 32.2%. assembled mitogenome, consisting 14,260 bp, unique 13 protein-coding genes (PCGs), 22 transfer RNAs, two ribosomal RNAs genes. common gene organization feature Chromodorididae. provides essential important DNA molecular data further...

Tail coiling is a reflection response in fish embryos that can be used as model for neurotoxic analysis. The previous method to analyze tail largely based on third-party software. In this study, we aim develop simple and cost-effective called TCMacro by using ImageJ macro reduce the operational complexity. basic principle of current dynamic change pixel intensity region interest (ROI). When moving, average increasing. time when freeze, peak mean maintaining at relatively low level. By...

Abstract Metabolic reprogramming is a pivotal characteristic of cancer, yet the intricate interplay between glycolysis and pentose phosphate pathway (PPP) remains elusive. This study unveils role 6-phosphofructokinase liver type (PFKL) in ribose 5-phosphate isomerase A (RPIA) PPP, orchestrating tumorigenesis. PFKL, rate-limiting enzyme glycolysis, stabilizes RPIA by impeding ubiquitination/proteasome activity. The pro-inflammatory tumor cytokine interleukin 6 activates pSTAT3 which binds to...

Abstract Carassius auratus complex formula (CACF) is a traditional Chinese medicine known for its antidiabetic effects. Hepatocellular carcinoma (HCC) major cause of cancer‐related deaths worldwide, and there are currently no effective therapies advanced HCC. This study explores the comprehensive effects possible mechanisms CACF on The results show that reduces viability hepatoma cells in vitro, while benefiting normal hepatocytes. In addition, inhibits cell growth zebrafish...