A5061976606- Pneumonia and Respiratory Infections
- Influenza Virus Research Studies
- Respiratory viral infections research
- Immune Response and Inflammation
- Neonatal Respiratory Health Research
- Hematopoietic Stem Cell Transplantation
- Antibiotic Resistance in Bacteria
- Bacterial Infections and Vaccines
- Effects of Radiation Exposure
- Bacterial Genetics and Biotechnology
- Asthma and respiratory diseases
- RNA and protein synthesis mechanisms
- Streptococcal Infections and Treatments
- Cellular transport and secretion
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neonatal Health and Biochemistry
- Erythrocyte Function and Pathophysiology
- Respiratory Support and Mechanisms
- Immunodeficiency and Autoimmune Disorders
- Pediatric health and respiratory diseases
- Polyomavirus and related diseases
- Neuroscience of respiration and sleep
Karolinska Institutet
2014-2023
Karolinska University Hospital
2023
Research Center Borstel - Leibniz Lung Center
2010-2013
University of Rostock
2009
Genome delivery to the proper cellular compartment for transcription and replication is a primary goal of viruses. However, methods analyzing viral genome localization differentiating genomes with high identity are lacking, making it difficult investigate entry-related processes co-examine heterogeneous RNA populations. Here, we present an labeling approach single-cell analysis co-infection dynamics in situ, which uses versatility padlock probes. We applied this method identify influenza A...
Influenza A virus (IAV) and Streptococcus pneumoniae are major causes of respiratory tract infections, particularly during coinfection. The synergism between these two pathogens is characterized by a complex network dysregulated immune responses, some which last until recovery following IAV infection. Despite the high serotype diversity S. replacement observed since introduction conjugate vaccines, little known about pneumococcal strain dependency in enhanced susceptibility to severe...
Streptococcus pneumoniae evades C3-mediated opsonization and effector functions by expressing an immuno-protective polysaccharide capsule Factor H (FH)-binding proteins. Here we use super-resolution microscopy, mutants functional analysis to show how these two defense mechanisms are functionally spatially coordinated on the bacterial cell surface. We that pneumococcal is less abundant at wall septum, providing C3/C3b entry underlying nucleophilic targets. Evasion of C3b deposition division...
Significance Mechanisms for why influenza A virus (IAV) infections sensitize pneumococcal are not clear. Here, we show that IAV-induced capillary leakage results in influx of nutrients and antioxidants to the lungs, thereby promoting growth lower respiratory tract. The evoked inflammation leads redox imbalances require bacterial adaptation oxidized environment, including induction chaperone/protease HtrA protects bacteria from clearance by immune system. give us insight into delicate...
ABSTRACT A functional immune response is crucial to prevent and limit infections with Streptococcus pneumoniae . Dendritic cells (DCs) play a central role in orchestrating the adaptive innate responses by communicating other cell types via antigen presentation secretion of cytokines. In this study, we set out understand how pneumococci activate human monocyte-derived DCs produce interleukin-12 (IL-12) p70, an important cytokine during pneumococcal infections. We show that IL-12p70 production...
The soluble C-type lectin surfactant protein (SP)-A mediates lung immune responses partially via its direct effects on alveolar macrophages (AM), the main resident leukocytes exposed to antigens. SP-A modulates AM threshold of lipopolysaccharide (LPS) activity towards an anti-inflammatory phenotype both in vitro and vivo through various mechanisms. LPS are tightly regulated distinct pathways including subcellular TLR4 localization thus ligand sensing. cytosolic scaffold signaling β-arrestin...
Abstract Surfactant protein A (SP-A), the most abundant pulmonary soluble collectin, modulates innate and adaptive immunity of lung, partially via its direct effects on alveolar macrophages (AM), predominant intra-alveolar cells under physiological conditions. Enhanced phagocytosis endocytosis are key functional consequences AM/SP-A interaction, suggesting a SP-A–mediated modulation small Rab (Ras related in brain) GTPases that pivotal membrane organizers both processes. In this article, we...
Treosulfan (Treo) and total body irradiation (TBI) demonstrate a high therapeutic activity in treatment of acute leukemia lymphoma. We investigated the combination Treo TBI prior to bone marrow transplantation (BMT) rats. Female Lewis rats were treated with on 3 consecutive days followed by either 5 Gy (n = 28) or 7.5 48). After conditioning animals received 4 × 10E7 cells (BC) from female Additional 16 transplanted BC 1.5 spleen T-cells Brown-Norway (BN) Animals examined daily for clinical...
Abstract Staphylococcus aureus is a significant cause of morbidity and mortality in pulmonary infections. Patients with autosomal-dominant hyper-IgE syndrome due to STAT3 deficiency are particularly susceptible acquiring staphylococcal pneumonia associated lung tissue destruction. Because macrophages involved both pathogen defense inflammation, we investigated the impact murine myeloid on macrophage phenotype vitro clearance inflammation during pneumonia. Murine bone marrow–derived (BMDM)...
Angeborene und erworbene Immunantworten in der Lunge werden durch das pulmonale Collectin Surfactant Protein (SP)-A moduliert. Vorarbeiten aus unserem Labor haben gezeigt, dass die endozytotische Aufnahme von SP-A eine Voraussetzung für SP-A-vermittelte Stabilisierung IκB-α, dem vorherrschenden Inhibitor NFκB ist. Zur Aufklärung daran beteiligten Mechanismen, wurden Effekte auf kleinen Rab GTPasen, Rab7 Rab7b untersucht. Die GTPasen essentielle Rolle im exo- endozytotischen...
Abstract Streptococcus pneumoniae is a commensal of the human nasopharynx, but it can also cause severe life-threatening antibiotic-resistant infections. Antibiotic consumption drives spread resistance by inducing S. competence leading to uptake exogenous DNA and horizontal gene transfer (HGT). We have identified potent inhibitors competence, collectively called COM-blockers. show that COM-blockers inhibit HGT perturbing proton motive force, thereby disrupting export peptide regulates...