A5078603467- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Sperm and Testicular Function
- Reproductive System and Pregnancy
- Animal Genetics and Reproduction
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- Pluripotent Stem Cells Research
- Biological Stains and Phytochemicals
- Bioactive Compounds in Plants
- Bioenergy crop production and management
- Microbial Metabolism and Applications
- Reproductive Health and Technologies
- MicroRNA in disease regulation
- Birth, Development, and Health
Universidad de Murcia
2021-2025
Instituto Murciano de Investigación Biosanitaria
2022-2024
The Superfine Open Pulled Straw (SOPS) system is the most commonly used method for vitrification of pig embryos. However, this only allows four to seven embryos per straw. In study, we investigated effectiveness open (OC) and closed (CC) Cryotop ® systems simultaneously vitrify a larger number porcine Morulae, early blastocysts full were vitrified with ( n = 250; 20 device) system, 158; traditional superfine pulled straw (SOPS; 241; 4–7 straw) method. Fresh from each developmental stage...
The combination of estrus synchronization and superovulation treatments introduces molecular modifications whose effects are yet to be disclosed. Here, reproductive parameters gene expression changes in ovaries endometrium were explored on day 6 after artificial insemination (AI), when synthetic progestin altrenogest (ALT) was combined with gonadotropins. Sows administered ALT for 7 d beginning the weaning superovulated equine chorionic gonadotropin (eCG) 24 h later human gonadotropins (hCG)...
This study examined how the vitrification of pig blastocysts using either superfine open-pulled straw (SOPS) or Cryotop method affects expression profile embryonic microRNA (miRNA) transcriptomes, as well its relation to changes in target genes (TGs). Surgically collected were vitrified SOPS (n = 60; 4-6 embryos/device) system 20 embryos/device). Embryos cultured vitro for 24 h after warming. Fresh 60) hours served controls. After culture, five pools eight viable from each group prepared...
Although embryo transfer (ET) is a biotechnology ready for the swine industry, there are factors to be solved, availability of donors as one. Multiparous sows ought considered since weaning natural and efficient method estrus synchronization. In addition, superovulation treatments at effective in increasing efficiency donor production. However, ET programs typically require more than those available from single weaning, imposing grouping several weanings establish batch ET. Since short-term...
The most commonly used technique to vitrify pig embryos is the super open pulled straw (SOPS), where a maximum of 6 can be vitrified simultaneously per device without compromising minimum volume necessary for optimal preservation. Since embryo transfer (ET) demands 20-40 recipient, customary use SOPS complicates warming and ET in field conditions. Such complications could avoided when using Cryotop® (OC) system, which has been proven an effective option vitrifying at least 20 porcine...
The combination of estrus synchronization and superovulation (SS) treatments causes alterations in ovarian endometrial gene expression patterns, resulting abnormal follicle oocyte growth, fertilization, embryo development. However, the impact combined SS on transcriptome surviving embryos remains unidentified. In this study, we examined changes day 6 blastocysts that survived a brief regimen treatment with superovulation. sows were included one three groups: SS7 group (n = 6), administered...
Despite the reported promising farrowing rates after non-surgical and surgical transfers of vitrified porcine morulae blastocysts produced in vivo (range: 70–75%), pregnancy loss is 5–15 fold higher with than fresh embryos. The present study aimed to investigate whether vitrification affects transcriptome morulae, using microarrays RT-qPCR validation. Morulae were obtained surgically from weaned sows ( n = 13) on day 6 (day 0 estrus onset). A total 60 (treatment group). After 1 week storage,...
Introduction Pregnancy success relies on the establishment of a delicate immune balance that requires early activation series local and systemic mechanisms. The changes in immunological profile are normally occurring pregnant uterus does not take place cyclic (non-pregnant) uterus, fact has been widely explored pigs at tissue level. Such differences would be especially important context embryo transfer (ET), where growing body literature indicates uterine level between donors recipients may...
Abstract This study aimed to compare the effectiveness, in terms of viability and quality post‐warming, when vitrifying vitro‐produced (IVP) pig blastocysts with either a modified Cryotop ( n = 161; 20 blastocysts/device) or conventional Superfine Open Pulled Straw (SOPS; =160; 5‐6 blastocysts/device systems. Blastocyst viability, morphology, apoptosis parameters were evaluated after 24 h vitro culture. The system yields better results higher embryo total cell numbers p < .05) lower than...
The aims of this study were to investigate the effects different equilibration times with cryoprotectants on viability and metaphase plate morphology vitrified-warmed porcine mature oocytes (Experiment 1) evaluate supplementation 10-9 M melatonin during in vitro maturation these parameters 2). In Experiment 1, 2,392 vitrified using (3, 10, 15, 20, 30, 40, 60 80 min). Fresh matured for 44 hr (n = 509) used as controls. 2, a total 573 COCs used. or without (control). Some from each group...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife