Uridine diphosphate (UDP)-glucosyltransferases (UGTs) have received increasing attention in the field of ginsenoside Rh2 conversion. By harnessing metal chelation between transition ions and imidazole groups present on His-tagged enzymes, a specific immobilization enzyme within metal–organic frameworks (MOFs) is achieved. This innovative approach not only enhances stability reusability but also enables one-step purification immobilization. Consequently, need for purifying crude solutions...

Enhancing the stability of multienzyme cascade reactions in metal-organic frameworks (MOFs) is a challenging task fields biotechnology and chemistry. However, addressing this challenge could yield far-reaching benefits across application range biomedical, food, environmental sectors. In study, partitioning immobilization that sequentially immobilizes enzymes with hierarchical MOFs proposed to reduce substrate diffusion resistance. Conversion results ginsenosides indicate strategy improves...

Timeline generation is of great significance for a comprehensive understanding the development events over time. Its goal to organize news chronologically, which helps identify patterns and trends that may be obscured when viewing in isolation, making it easier track stories understand interrelationships between key events. Timelines are now common various commercial products, but academic research this area notably scarce. Additionally, current datasets need refinement enhanced utility...

The use of microbial fertilizers can help to avoid the harmful effects traditional agricultural and pesticides; however, there are many constraints on practical application such fertilizers. In this study, biochar formulations (MBFs) were obtained by loading biochar, created from waste, with Bacillus subtilis SL-13. MBF pepper plant growth soil fertility studied in pot experiments. improved texture environment favored growth. Compared B. SL-13-only biochar-only treatments, treatments...

This research focuses on the transformation of classroom teaching within local universities during this era rapid change. It lays stress incorporation industry, profession, and vocation aspects so as to tackle crucial matters like narrowing disparity between instructional content enterprise advancements optimizing pathways cooperation. The investigation puts forward a variety pedagogical strategies, namely practical case-based pedagogy, interactive modalities, group deliberation approaches,...

The diamondback moth (Plutella xylostella) exhibits significant resistance to commonly used insecticides including λ-cyhalothrin. Delta-class glutathione S-transferases (GSTs) are crucial detoxification enzymes involved in insecticide and resistance. We demonstrate that PxGSTD3 is associated with the λ-cyhalothrin contributes detoxification. transcription of was rapidly upregulated response exposure, recombinant protein exhibited metabolic activity against Further investigation using...

Biochar is a biomaterial obtained by pyrolysis with high porosity and specific surface area (SSA), which widely used in several fields. The yield of biochar has an important effect on production cost utilization efficiency, while SSA plays key role adsorption, catalysis, pollutant removal. preparation materials better currently one the frontiers this research field. However, traditional methods are time consuming laborious, so paper developed machine learning model to predict study...

Glycyrrhetinic acid (GA) is the pentacyclic triterpenoid hydrophobic aglycone with many pharmacological effects and biological activities. Glycosylation often used to improve aglycone's properties such as solubility, stability, potency. UDP-glycosyltransferases (UGTs) are main enzymes catalyze this conversion via transferring glycosyl moiety corresponding acceptor substrates in nature. However, a glycosyltransferase which can transfer glucose GA has not been reported yet. The UGT73C11 from...

Conventional photocatalysts for degrading pollutants are limited because of the rapid recombination electron–hole pair, difficulty in separation and recovery, low efficiency utilizing visible light. To improve adsorption capacity photocatalyst reduce rate, βCD was anchored onto porous activated carbon (AC) then combined with ZnO/ZnSe to prepare βCD‐AC‐ZnO/ZnSe. The ZnO a quasi‐hexahedron shape heterojunction tightly integrated βCD‐AC, which could transfer ability photogenerated carrier....

Biochar-based bacteria have a significant promoting effect on plant growth, but their effectiveness in improving saline–alkali soils still needs to be improved. In order fill these aspects, modified biochar was obtained by copyrolysis of Ca3(PO4)2, MgO, and biomass cocultured with Enterobacter hormaechei Wu15. The surface became rough after the addition Ca3(PO4)2 Brunauer–Emmett–Teller area increased. ζ potential showed that more Wu15 could adhere due existence electrostatic interaction, so...

Chemical fertilizers were applied on perennial tree vines to obtain high yields, which have resulted in considerable deterioration of soil quality, and it is likely negative impacts the development grape industry. In this study, P. putida Rs-198 liquid biofertilizer (Rs198LBF) was inoculated into rhizosphere soils assess its influence growth microbial community. Field experiment results showed that quality increased depending concentrations Rs198LBF applications. The berry weight, length,...

ABSTRACT The biological conversion method using immobilized enzymes has become an important means to produce ginsenosides. Among them, the preprepared Zn‐Ni MOF could be used immobilize glycosyltransferase by specific adsorption for ginsenoside Rh2 production. However, low enzyme load of limited its wide application. In this study, we successfully synthesized Glu@Zn‐Ni filled with nanospheres modifying previously prepared glutamic acid (Glu) through ligand exchange. This modification...

Dissolved organic matter (DOM) released from biochar may impact antibiotic mobility and environmental fate in subsurface environments. Here, DOM samples derived biochars (BDOM) generated by pyrolyzing corn straw at 300, 450, 600 °C were employed to elucidate the characteristics of these substances their influences on transport sulfamerazine (SMZ, a typical sulfonamide antibiotic) soil porous media. The results demonstrated that BDOM produced lower pyrolysis temperature exhibited greater...

Optimal decision making requires appropriate evaluation of advice. Recent literature reports that algorithm aversion reduces the effectiveness predictive algorithms. However, it remains unclear how people recover from bad advice given by an otherwise good advisor. Previous work has focused on at a single time point. We extend this examining successive decisions in series forecasting task using online between-subjects experiment (N = 87). Our empirical results do not confirm immediately after...

Traditional metal-organic frameworks (MOFs) preparation is generally time-consuming, polluting, and lacking specificity for enzyme immobilization. This paper introduced a facile, rapid, green method to produce three MOFs subsequently employed purify coimmobilize recombinant glycosyltransferase (UGT) sucrose synthetase (SUSy) using histidine tag (His-tag) the specific adsorption of Ni

Recently invented super-aligned carbon nanotube (CNT) array is a remarkable achievement in modern science. Its unique, highly ordered structure meaningful and applicable many fields. We report fluorescent analysis of this functioning as template to arrange the stretched, YOYO-1 labeled λDNAs. Real time imaging results showed that stretched λDNAs on CNT films laid along direction arrays. This work demonstrates arrays are useful nano-manipulation biomacromolecules, indicates further...

Abstract Ginsenoside Compound K (CK) has high pharmacological activity for being widely used in the treatment and prevention of diseases. However, content CK ginseng is very low or almost non-existent. In present study, ginsenoside Rb1 was converted into F2 by using extracellular protein isolated from Aspergillus niger Wu-16. The results show that crude enzymes produced Wu-16 can catalyze conversion substrate to CK. reaction time, pH, temperature, ratio enzyme preparing were as optimization...