A5042946413- Bacterial Identification and Susceptibility Testing
- Burkholderia infections and melioidosis
- Signaling Pathways in Disease
- Peptidase Inhibition and Analysis
- Toxin Mechanisms and Immunotoxins
- Research on Leishmaniasis Studies
- Antimicrobial Resistance in Staphylococcus
- Streptococcal Infections and Treatments
- Trypanosoma species research and implications
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Clostridium difficile and Clostridium perfringens research
- Galectins and Cancer Biology
- Enterobacteriaceae and Cronobacter Research
- Calcium signaling and nucleotide metabolism
- Amoebic Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Antimicrobial Peptides and Activities
- Antibiotics Pharmacokinetics and Efficacy
The University of Western Australia
2015-2024
Queen Elizabeth II Medical Centre
2018
Pathwest Laboratory Medicine
2015
The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation resistance and after initial isolation bacteria from a clinical specimen. Here we describe flow cytometry workflow determine carbapenem bacterial cell characteristics in an international K. isolate collection (n = 48), with range carbapenemases. Our cytometry-assisted test (FAST)...
<title>Abstract</title> Group A <italic>Streptococcus</italic> (GAS) has remained universally susceptible to penicillin since the introduction of this antibiotic treat GAS throat infections (“Strep throat”) in 1940’s. Despite this, a proportion Strep fail completely resolve with therapy, recurrent requiring follow-up treatment alternative broad-spectrum antibiotics. Here we show that strains isolated from amoxicillin failures are able invade into epithelial cells and replicate presence...
The facultative anaerobic Gram-negative species Escherichia albertii has been isolated from human faeces in gastrointestinal infection and a range of wild bird species. Here we report the first case febrile associated with E. bacteraemia 76-year-old woman gastric dysplasia.
Coxiella burnetii is a Gram-negative intracellular pathogen that causes the debilitating disease Q fever, which affects both animals and humans. The only available human vaccine, Q-Vax, effective but has high risk of severe adverse reactions, limiting its use as countermeasure to contain outbreaks. Therefore, it essential identify new drug targets treat this infection. Macrophage infectivity potentiator (Mip) proteins catalyse folding proline-containing through their peptidyl prolyl...
Abstract Background The macrophage infectivity potentiator (Mip) protein, which belongs to the immunophilin superfamily, is a peptidyl-prolyl cis/trans isomerase (PPIase) enzyme. Mip has been shown be important for virulence in wide range of pathogenic microorganisms. It previously demonstrated that small-molecule compounds designed target from Gram-negative bacterium Burkholderia pseudomallei bind at site enzymatic activity inhibiting vitro Mip. Objectives In this study, co-crystallography...
The macrophage infectivity potentiator (Mip) protein is a promising target for developing new drugs to combat antimicrobial resistance. New rapamycin-derived Mip inhibitors have been designed that may be able combine two binding modes inhibit the of Burkholderia pseudomallei (BpMip). These novel compounds are characterized by an additional substituent in middle chain linking lateral pyridine pipecoline moiety, constituting different stereoisomers. demonstrated high affinity BpMip nanomolar...
Burkholderia pseudomallei is the causative agent of melioidosis, a disease endemic to Southeast Asia and northern Australia. Mortality rates in these areas are high even with antimicrobial treatment, there few options for effective therapy. Therefore, need identify antibacterial targets development novel treatments. Cyclophilins family highly conserved enzymes important multiple cellular processes. catalyze cis-trans isomerization xaa-proline bonds, rate-limiting step protein folding which...
The treatment of Chagas disease and infections with Gram-negative bacteria is limited to a low number antibiotics. Due the development resistance partially severe side effects, there an urgent need for new strategies virulence factors such as macrophage infectivity potentiator (MIP) protein have emerged promising therapeutic target. Inhibition microbial MIP proteins leads reduced viability proliferation in pathogens Legionella pneumophila Burkholderia pseudomallei. parasitic pathogen...
Since Chagas disease, melioidosis, and Legionnaires' disease are all potentially life-threatening infections, there is an urgent need for new treatment strategies. All causative agents,
Rapid identification of bacteria isolated from blood cultures by direct matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now in wide spread use major centres but not yet feasible smaller hospital laboratories. A FilmArray multiplex PCR panel for culture isolate (BCID) provides an alternative approach to near point-of-care microbial regional hospitals. We assessed the accuracy and time BCID a consecutive series 149 143 patients teaching hospitals,...
Purpose. In this field trial of rapid blood culture identification (BCID), we aimed to determine whether the improved speed and accuracy specific BCID predicted in our earlier pilot study could be obtained regional hospitals by deploying a multiplex PCR FilmArray (Biomerieux, France) capability their laboratories.Methods. We trained local hospital laboratory staff operate equipment act on results. To do this, integrated into standard workflow reporting procedure.Results. Of 100 positive...
Introduction Melioidosis, caused by the Gram-negative bacterium Burkholderia pseudomallei , is a disease endemic in many tropical countries globally. Clinical presentation highly variable, ranging from asymptomatic to fatal septicemia, and thus outcome of infection can depend on host immune responses. The aims this study were firstly, characterize macrophage response B. secondly, determine whether was modified presence novel inhibitors targeting virulence factor, infectivity potentiator...