Yi‐Wen Lim

ORCID: 0000-0003-2213-5777
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About
Contact & Profiles
Research Areas
  • Cassava research and cyanide
  • Nematode management and characterization studies
  • Oil Palm Production and Sustainability
  • Plant Virus Research Studies
  • Banana Cultivation and Research
  • Medicinal Plants and Neuroprotection
  • Legume Nitrogen Fixing Symbiosis

ETH Zurich
2021-2022

Abstract Background Cassava (Manihot esculenta) is an important clonally propagated food crop in tropical and subtropical regions worldwide. Genetic gain by molecular breeding has been limited, partially because cassava a highly heterozygous with repetitive difficult-to-assemble genome. Findings Here we demonstrate that Pacific Biosciences high-fidelity (HiFi) sequencing reads, combination the assembler hifiasm, produced genome assemblies at near complete haplotype resolution higher...

10.1093/gigascience/giac028 article EN cc-by GigaScience 2022-01-01

Abstract Cassava mosaic disease (CMD) suppresses cassava yields across the tropics. The dominant CMD2 locus confers resistance to geminiviruses. It has been reported that CMD2-type landraces lose after regeneration through de novo morphogenesis. As full genome bisulfite sequencing failed uncover an epigenetic mechanism for this loss of resistance, whole and genetic variant analysis was performed fine-mapped a 190 kilobase interval. Collectively, these data indicate is caused by...

10.1038/s41467-022-31414-0 article EN cc-by Nature Communications 2022-07-07

Cytosine DNA methylation is involved in transposable element (TE) silencing, imprinting and X-chromosome inactivation. Plant mediated by MET1 (mammalian DNMT1), DRM2 DNMT3) two plant-specific methyltransferases, CMT2 CMT3 (Law Jacobsen, 2010). De novo plants established via the RNA-directed (RdDM) pathway that depends on DNA-dependent RNA polymerases, Pol IV V (Gallego-Bartolome et al., 2019; Law 2010; Stroud 2013). The methylome of cassava has been previously documented based its haploid...

10.1111/pbi.13955 article EN cc-by Plant Biotechnology Journal 2022-11-01

Abstract Background Cassava ( Manihot esculenta ) is an important clonally propagated food crop in tropical and sub-tropical regions worldwide. Genetic gain by molecular breeding limited because cassava has a highly heterozygous, repetitive difficult to assemble genome. Findings Here we demonstrate that Pacific Biosciences high-fidelity (HiFi) sequencing reads, combination with the assembler hifiasm, produced genome assemblies at near complete haplotype resolution higher continuity accuracy...

10.1101/2021.11.16.468774 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2021-11-19

ABSTRACT Cassava mosaic disease suppresses cassava yields across the tropics. The dominant CMD2 locus confers resistance to geminiviruses. It has been reported that CMD2-type landraces lose after regeneration through de novo morphogenesis. As full genome bisulfite sequencing failed uncover an epigenetic mechanism for loss of resistance, we performed whole and genetic variant analysis fine-mapped a 190 kilobase interval. Data suggest is caused by nonsynonymous, single nucleotide polymorphism...

10.1101/2022.04.13.487913 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2022-04-13

Cytosine DNA methylation is involved in biological processes such as transposable element (TE) silencing, imprinting, and X chromosome inactivation. Plant mediated by MET1 (mammalian DNMT1), DRM2 DNMT3), two plant-specific methyltransferases, CMT2 CMT3 (Law Jacobsen, 2010). De novo plants established via the plant specific RNA-directed (RdDM) pathway that depends on DNA-dependent RNA polymerases, Pol IV V (Gallego-Bartolome et al ., 2019; Law 2010; Stroud 2013). The methylome of cassava has...

10.1101/2022.09.12.507674 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2022-09-15
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