A5064050892- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- 3D Printing in Biomedical Research
- Advanced Biosensing Techniques and Applications
- Bee Products Chemical Analysis
- Gold and Silver Nanoparticles Synthesis and Applications
- Single-cell and spatial transcriptomics
- Advanced MRI Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Electrochemical sensors and biosensors
- Optical Coherence Tomography Applications
Osaka University
2022-2024
National Institute of Advanced Industrial Science and Technology
2022-2023
Although investigating drug modulation of cytochrome P450 (CYP) activity under physiological conditions is crucial in development to avoid severe adverse reactions, the current evaluation approaches that rely on destructive and end-point analysis can be misleading due invasive treatments cellular heterogeneity. Here, we propose a non-destructive high-content method for visualizing quantifying intracellular CYP administration by Raman microscopy. The redox-state spin-state sensitive...
In recent years, Raman microscopy has emerged as a molecular imaging tool for cell and tissue analysis. A key reason this is the development of techniques that significantly increase speed. Tutorial, we introduce slit-scanning microscopy, technique achieves speeds more than two orders magnitude faster conventional confocal its application to Recent advances in imaging, particularly further improvements speed, sensitivity, specificity, spatial resolution, are also discussed. addition, present...
Raman microscopy is an emerging molecular imaging technology, yet its signal-to-noise ratio (SNR) in measurements of biological specimens severely limited because the small cross section scattering. Here, we present techniques cryofixed to overcome SNR limitations by enabling long exposure under highly stabilized low-temperature conditions. The observation frozen a cryostat at constant low temperature immediately after rapid freezing enabled improvement and enhanced spatial spectral...
Cell spheroids offer alternative in vitro cell models to monolayer cultured cells because they express complexities similar those of vivo tissues, such as cellular responses drugs and chemicals. Raman spectroscopy emerged a powerful analytical tool for detecting chemical changes living it nondestructively provides vibrational information regarding target. Although multiple iterations are required drug screening determine treat assess the inter-spheroid heterogeneity, current applications...
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Raman flow cytometry offers chemically sensitive, label-free measurement of cells and particles; however, the technique suffers from low cell throughput due to weak signal. Here, we demonstrate use time-delay integration achieve combined with dual-sided line illumination. The illumination both sides capillary kept stream in detection area by balancing optical force lines. allowed accumulation signals flowing without sacrificing spectrum readout rate. With developed system, achieved at...
We developed a Raman spectrophotometer with multi-foci for high-throughput screening that produces 96 focal spots on analytes in well-plate and collects their spectra simultaneously, which enables fast discrimination monitoring of samples.
We developed a Raman spectrophotometer with multi-foci for high-throughput screening that produces 96 focal spots on analytes in well-plate and collects their spectra simultaneously, which enables fast discrimination monitoring of96 samples.