A5055147992- Bacterial biofilms and quorum sensing
- Mast cells and histamine
- Infective Endocarditis Diagnosis and Management
- Allergic Rhinitis and Sensitization
- Systemic Sclerosis and Related Diseases
- RNA modifications and cancer
- Ion Channels and Receptors
- Powdery Mildew Fungal Diseases
- Biochemical and Structural Characterization
- Pneumocystis jirovecii pneumonia detection and treatment
- Biochemical Analysis and Sensing Techniques
- Antimicrobial Resistance in Staphylococcus
- Cell Adhesion Molecules Research
- Congenital heart defects research
- Cellular transport and secretion
- Vibrio bacteria research studies
- Ion channel regulation and function
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Synthesis and Biological Evaluation
- Venomous Animal Envenomation and Studies
- Genetics and Neurodevelopmental Disorders
- melanin and skin pigmentation
- Bacterial Identification and Susceptibility Testing
- Olfactory and Sensory Function Studies
- Erythrocyte Function and Pathophysiology
Princeton University
2021-2025
National Human Genome Research Institute
2018-2022
National Institutes of Health
2018-2021
Fluid flows are dominant features of many bacterial environments, and flow can often impact behaviors in unexpected ways. For example, the most common type cardiovascular infection is heart valve colonization by gram-positive bacteria like Staphylococcus aureus Enterococcus faecalis (endocarditis). This behavior counterintuitive because valves experience high shear rates that would naively be expected to reduce colonization. To determine whether these preferentially colonize higher rate we...
In Staphylococcus aureus, virulence is under the control of a quorum sensing (QS) circuit encoded in accessory gene regulator (agr) genomic locus. Key to this pathogenic behavior production and signaling activity secreted pheromone, autoinducing peptide (AIP), generated following ribosomal synthesis posttranslational modification precursor polypeptide, AgrD, through two discrete cleavage steps. The integral membrane protease AgrB known catalyze first processing event, generating AIP...
ConspectusMethodological development in the fields of genetics, chemical biology, and biochemistry over last several decades has provided researchers with a diverse set powerful tools to investigate biological processes. Leveraging these innovations concert, scientists can now characterize pathways at level complexity ranging from systems biology down molecular atomic detail.Throughout this Account, we illustrate how discoveries made using build on each other develop comprehensive...
ATP-binding cassette (ABC) transporters are evolutionarily conserved membrane proteins that pump a variety of endogenous substrates across cell membranes. Certain subfamilies known to interact with pharmaceutical compounds, potentially influencing drug delivery and treatment efficacy. However, the role resistance-associated ABC has not been examined in idiopathic pulmonary fibrosis (IPF) or its animal model: bleomycin (BLM)-induced murine model. Here, we investigate expression two...
Hermansky-Pudlak syndrome (HPS) is an autosomal recessive disorder characterized by improper biogenesis of lysosome-related organelles (LROs). Lung fibrosis the leading cause death among adults with HPS-1 and HPS-4 genetic types, which are associated defects in complex-3 (BLOC-3), a guanine exchange factor (GEF) for small GTPase, Rab32. LROs not ubiquitously present all cell specific cells utilize to accomplish dedicated functions. Fibroblasts known contain LROs, function BLOC-3 fibroblasts...
Abstract Background HPS-1 is a genetic type of Hermansky-Pudlak syndrome (HPS) with highly penetrant pulmonary fibrosis (HPSPF), restrictive lung disease that similar to idiopathic (IPF). Hps1 ep/ep (pale ear) naturally occurring mouse model exhibits high sensitivity bleomycin-induced (PF). Traditional methods administering bleomycin as an intratracheal (IT) route induce PF in this often lead severe acute injury and mortality rates, complicating studies focusing on pathobiological mechanisms...
Abstract In Staphylococcus aureus , virulence is under the control of a quorum sensing (QS) circuit encoded in accessory gene regulator ( agr ) genomic locus. Key to this pathogenic behavior production and signaling activity secreted pheromone, autoinducing peptide (AIP), generated following ribosomal synthesis post-translational modification precursor polypeptide, AgrD, through two discrete cleavage steps. The integral membrane protease AgrB known catalyze first processing event, generating...
Abstract Fluid flows can impact bacterial behaviors in unexpected ways ( 1 – 3 ). The high shear rate heart valves should reduce colonization, but endocarditis, are often counter-intuitively colonized by Staphylococcus aureus and Enterococcus faecalis 4, 5 Here we discover bacteria-specific mechanisms for preferential surface colonization higher environments. This behavior enables bacteria that outcompeted low flow to dominate flow. Flow-dependent S. E. mediated distinct depend on each...