A5102776566- Adenosine and Purinergic Signaling
- Advanced Electron Microscopy Techniques and Applications
- Chromosomal and Genetic Variations
- Neurological Complications and Syndromes
- Molecular Biology Techniques and Applications
- Genetic Mapping and Diversity in Plants and Animals
- Neuroinflammation and Neurodegeneration Mechanisms
- Genomics and Chromatin Dynamics
- Advanced Fluorescence Microscopy Techniques
- Pluripotent Stem Cells Research
- Near-Field Optical Microscopy
- RNA Interference and Gene Delivery
University of Illinois Chicago
2024
Loyola University Chicago
2023
Stony Brook University
1983
State University of New York
1983
Population Council
1983
Adenosine triphosphate (ATP) and its metabolites drive microglia migration cytokine production by activating P2X- P2Y- class purinergic receptors. Purinergic receptor activation gives rise to diverse intracellular calcium (Ca2+ signals, or waveforms, that differ in amplitude, duration, frequency. Whether how these characteristics of waveforms influence function is not well-established. We developed a computational model trained with data from published primary murine studies. simulate...
A dramatic difference is observed in the intracellular distribution of high mobility group (HMG) proteins when chicken embryo fibroblasts are fractionated into nucleus and cytoplasm by either mass enucleation cytochalasin-B-treated cells or differential centrifugation mechanically disrupted cells. Nuclei (karyoplasts) obtained cytochalasin B treatment contain more than 90 percent HMG 1, while enucleated cytoplasts remainder. similar between karyoplasts for H1 histones nucleosomal core as...
ABSTRACT We present single-molecule labeling and localization microscopy (SMLLM) using dye-conjugated phalloidin to achieve enhanced superresolution imaging of filamentous actin (F-actin). demonstrate that the intrinsic dissociation enables SMLLM in an buffer containing low concentrations phalloidin. further show by chemically promoting dissociation. Two benefits phalloidin-based are better preservation cellular structures sensitive mechanical shear forces during standard sample preparation...
Reconstituted cells were constructed by fusion of cytoplasts from the human diploid fibroblast cell strain Detroit 532 and karyoplasts mouse line A9. Several cellular properties examined during first 48 hr after nuclear transplantation. (i) The overall morphology originally resembled that cytoplasmic donor, 532, but rapidly changed to approximate However, definitive changes in microfilament structure reconstituted not seen until 24-48 fusion. These observations support idea presence or...