A5059563933- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Contact Dermatitis and Allergies
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Hormonal Regulation and Hypertension
- Polyamine Metabolism and Applications
- Advanced biosensing and bioanalysis techniques
- Plant-Microbe Interactions and Immunity
- Parasitic Infections and Diagnostics
- Metabolomics and Mass Spectrometry Studies
- Skin and Cellular Biology Research
- Cardiovascular Function and Risk Factors
- Dermatology and Skin Diseases
- Plant Virus Research Studies
- Adipose Tissue and Metabolism
- Transgenic Plants and Applications
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Mitochondrial Function and Pathology
- Advanced Proteomics Techniques and Applications
- Plant Disease Resistance and Genetics
- Cancer and Skin Lesions
- RNA modifications and cancer
- DNA and Nucleic Acid Chemistry
Universität Innsbruck
2018-2024
Karolinska Institutet
2022-2024
PR-10 proteins constitute a major cause of food allergic reactions. Birch-pollen-related allergies are triggered by the immunologic cross-reactivity IgE antibodies with structurally homologous that present in birch pollen and various sources. While three-dimensional structures allergens have been characterized detail, only few experimental studies addressed structural flexibility these proteins. In this study, we analyze millisecond-timescale thirteen from prevalent plant sources NMR...
Naturally occurring polyphenols can modify the molecular properties of food allergens. For major apple allergen Mal d 1 it has been postulated that chemical reactions with cause permanent changes in tertiary structure, causing a loss conformational IgE epitopes and reducing allergenicity. In our study, we investigated effect oxidized have on structure by mass spectrometry NMR spectroscopy. We showed surface-exposed cysteine residue this spontaneously reacts under formation defined covalent...
The Sjögren–Larsson syndrome (SLS) is a rare autosomal recessive disorder caused by pathogenic variants in the ALDH3A2 gene, which codes for fatty aldehyde dehydrogenase (FALDH). FALDH prevents accumulation of toxic aldehydes converting them into acids. Pathogenic cause symptoms such as ichthyosis, spasticity, intellectual disability, and wide range less common clinical features. Interpreting patient-to-patient variability often complicated inconsistent reporting negatively impacts on...
Seventy percent of patients suffering from birch pollen allergy (BPA) develop a pollen-related food (prFA), especially to apples, due clinically relevant cross-reactivity between the major allergen in Bet v 1 and Mal d apples. Therefore allergen-specific immunotherapy with fresh apples (AITA) could be promising natural treatment both BPA prFA.To assess clinical efficacy by daily apple consumption for prFA.A defined increasing amount selected cultivars (Red Moon®, Pink Lady®, Topaz, Golden...
Abstract Background Most birch pollen‐allergic patients develop allergic cross‐reactions to the major allergen found in apples Mal d1, known as pollen‐related food allergy (pr FA ). This is due a strong clinically relevant homology between Bet v 1 and d 1. Daily apple consumption induces oral tolerance pr , but its effect on inhalational has not been investigated. Objectives As continuous might also mitigate allergy, this study aimed uncover cultivars suitable for treatment of pollen...
The ProQ/FinO family of RNA binding proteins mediate sRNA-directed gene regulation throughout gram-negative bacteria. Here, we investigate the structural basis for recognition by proteins, through crystal structure domain Legionella pneumophila DNA uptake regulator, RocC, bound to transcriptional terminator its primary partner, sRNA RocR. reveals specific 3' nucleotide a conserved pocket involving β-turn-α-helix motif, while hairpin portion is recognized α-helical N-cap motif....
In microdroplets, rates of chemical or biomolecular reactions can exceed those in the bulk phase by more than a million times. As electrospray ionization-based mass spectrometry (MS) involves formation charged reaction acceleration and online MS monitoring products readily be performed at same time. We investigated accelerated enzymatic microdroplets focused on proteolytic enzyme pepsin. Electrosonic spray ionization (ESSI) was utilized for developing ultrarapid pepsin in-spray digestion two...
Peach (Prunus persica) is among the fruits most frequently reported to cause food allergies. Allergic reactions commonly result from previous sensitization birch pollen allergen Bet v 1, followed by immunological cross-reactivity of IgE antibodies structurally related proteins in peach. In this study, we present three-dimensional NMR solution structure cross-reactive peach Pru p 1 (isoform 1.0101). This 17.5 kDa protein adopts canonical fold, composed a seven-stranded β-sheet and three...
Abstract Understanding the structure and function of nucleic acids in their native environment is crucial to structural biology one focus in-cell NMR spectroscopy. Many challenges hamper human cell lines, e.g. sample decay through death RNA degradation. The resulting low signal intensities broad line widths limit use more complex experiments, reducing possible dynamic information that can be extracted. Here, we optimize detection imino proton signals, indicators base-pairing therefore...
Multifunctional proteins are challenging as it can be difficult to confirm pathomechanisms associated with disease-causing genetic variants. The human 17β-hydroxysteroid dehydrogenase 10 (HSD10) is a moonlighting enzyme at least two structurally and catalytically unrelated functions. HSD10 disease was originally described disorder of isoleucine metabolism, but the clinical manifestations were subsequently shown linked impaired mtDNA transcript processing due deficient function in mtRNase P...
The protein Mal d 1 is responsible for most allergic reactions to apples (
Pathogenesis-related class 10 (PR-10) proteins play a crucial role in plant defense by acting as ribonucleases. The specific mechanism of action and substrate specificity these have remained largely unexplored so far. In this study, we elucidate the enzymatic activity Pru p 1, PR-10 protein from peach. We demonstrate that catalyzes endonucleolytic backbone cleavage RNA substrates into short oligonucleotides. Initial products, identified through kinetic analysis, can bind again, priming them...
Abstract In prokaryotic species, gene expression is commonly regulated by small, non-coding RNAs (sRNAs). the gram-negative bacterium Legionella pneumophila , regulatory, trans-acting sRNA molecule RocR base pairs with a complementary sequence in 5’-untranslated region of mRNAs encoding for proteins bacterial DNA uptake system, thereby controlling natural competence. Sense-antisense duplexing targeted mediated recently described RNA chaperone RocC. RocC contains 12 kDa FinO-domain, which...