A5103002378- Carcinogens and Genotoxicity Assessment
- Skin and Cellular Biology Research
- Sulfur Compounds in Biology
- Pharmacogenetics and Drug Metabolism
- Indoor Air Quality and Microbial Exposure
- Mesenchymal stem cell research
- Computational Drug Discovery Methods
- Air Quality and Health Impacts
- Eicosanoids and Hypertension Pharmacology
- Alcohol Consumption and Health Effects
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- Heavy Metal Exposure and Toxicity
- Cancer Cells and Metastasis
- Prostate Cancer Treatment and Research
- Lymphatic System and Diseases
- Peptidase Inhibition and Analysis
- Proteoglycans and glycosaminoglycans research
- Protease and Inhibitor Mechanisms
- Molecular Biology Techniques and Applications
- PARP inhibition in cancer therapy
- Skin Protection and Aging
- Wound Healing and Treatments
- Metabolomics and Mass Spectrometry Studies
- Neonatal Respiratory Health Research
Karolinska Institutet
2008-2025
Turku Centre for Biotechnology
2009-2018
VTT Technical Research Centre of Finland
2008-2012
University of Turku
2009
Institute for Molecular Medicine Finland
2009
University of Helsinki
2009
In-Q-Tel
2008
National Cancer Institute
1981-1984
National Institutes of Health
1982
Abstract Purpose: To identify novel therapeutic opportunities for patients with prostate cancer, we applied high-throughput screening to systematically explore most currently marketed drugs and drug-like molecules their efficacy against a panel of cancer cells. Experimental Design: We carried out cell-based proliferation as primary end-point using library 4,910 small molecule compounds in four (VCaP, LNCaP, DU 145, PC-3) two nonmalignant epithelial cell lines (RWPE-1 EP156T). The EC50 values...
Cultured bronchial epithelial and fibroblastic cells from humans were used to study DNA damage toxicity caused by formaldehyde. Formaldehyde the formation of cross-links between proteins, single-strand breaks in DNA, inhibited resealing produced ionizing radiation. also unscheduled synthesis that occurs after exposure ultraviolet irradiation or benzo[a]pyrene diolexpoxide but at doses substantially higher than those required inhibit x-ray-induced breaks. Therefore, formaldehyde could exert...
Journal Article Cytotoxicity, thiol depletion and inhibition of O 6 -methylguanine-DNA methyltransferase by various aldehydes in cultured human bronchial fibroblasts Get access Hans Krokan, Krokan 1Laboratory Human Carcinogenesis, Division Cancer Etiology, National InstituteBethesda, MD 20892, USA Search for other works this author on: Oxford Academic PubMed Google Scholar Roland C. Grafstrom, Grafstrom 2Department Toxicology, Karolinska InstitutetS-10401, Sweden Kristina Sundqvist,...
AbstractRadiotherapy remains the backbone of head and neck cancer therapy but response is sometimes impeded by tumor radioresistance. Identifying predictive biomarkers radiotherapy a crucial step towards personalized therapy. The aim this study was to explore gene expression data in search squamous cell carcinoma (HNSCC).Microarray analysis performed on five lines with various intrinsic radiosensitivity, selected from panel 29 HNSCC lines. bioinformatics approach included Gene Ontology (GO)...
Abstract In vitro-based high-throughput screening (HTS) technology is applicable to hazard-based ranking and grouping of diverse agents, including nanomaterials (NMs). We present a standardized HTS-derived human cell-based testing protocol which combines the analysis five assays into broad toxic mode-of-action-based hazard value, termed Tox5-score. The overall includes automated data FAIRification, preprocessing score calculation. A newly developed Python module ToxFAIRy can be used...
Abstract The thiol redox status of cultured human bronchial fibroblasts has been characterized at various growth conditions using thiol‐reactive monobromobimane, with or without the combination dithiotreitol, a strong reducing agent. This procedure enabled measurement cellular content reduced glutathione (GSH), total equivalents, cysteine, cysteine protein sulfhydryls, disulfides, and mixed disulfides. Passage cells trypsin pertubs homeostasis causes 50% decrease in GSH content, whereas is...
Abstract The aim of the SEURAT‐1 (Safety Evaluation Ultimately Replacing Animal Testing‐1) research cluster, comprised seven EU FP7 Health projects co‐financed by Cosmetics Europe, is to generate a proof‐of‐concept show how latest technologies, systems toxicology and toxicogenomics can be combined deliver test replacement for repeated dose systemic toxicity testing on animals. strategy adopt mode‐of‐action framework describe toxicity, combining in vitro silico methods derive predictions vivo...
Normal prostate and some malignant cancer (PrCa) cell lines undergo acinar differentiation form spheroids in three-dimensional (3-D) organotypic culture. Acini formed by PC-3 PC-3M, less pronounced also other PrCa lines, spontaneously an invasive switch, leading to the disintegration of epithelial structures basal lamina, formation invadopodia. This demonstrates highly dynamic nature plasticity, balancing epithelial-to-mesenchymal transition against metastable differentiation. study assessed...
Journal Article DNA repair in human bronchial epithelial cells Get access Albert J. Fornace, Jr., Jr. Laboratory of Human Carcinogenesis, Cancer Intramural Program, Division Cause and Prevention, National InstituteBethesda, MD 20205, USA 1 Present address: Pathology, Biology Diagnosis, Institute. Search for other works by this author on: Oxford Academic PubMed Google Scholar John F. Lechner, Lechner Roland C. Grafstrom, Grafstrom Curtis Harris 2 Address correspondence to Harris, M.D.,...
Journal Article Metabolism of chemical carcinogens by cultured human and rat bladder epithelial cells Get access Herman Autrup, Autrup 1Laboratory Environmental Carcinogenesis, CopenhagenDenmark2Human Tissue Studies Section, Laboratory Experimental Pathology, National Cancer InstituteBethesda, MD 20205, USA Search for other works this author on: Oxford Academic PubMed Google Scholar Roland C. Grafstrom, Grafstrom 2Human Britta Christensen, Christensen CopenhagenDenmark Jorgen Kieler Volume...
Metabolism of exogenous glutathione was investigated in suspensions freshly isolated rat small-intestinal mucosal cells. The cells catalyzed the oxidation reduced (GSH) to disulfide (GSSG). Neither serine . borate nor methionine significantly influenced this reaction. Formed GSSG further metabolized as indicated by its disappearance from medium. Degradation stimulated and inhibited borate. Separation identification metabolites were achieved high performance liquid chromatography. results...
Abstract The metabolism of chemical carcinogens has been studied in cultured human bronchus, colon, duodenum, pancreatic duct, and esophagus. Metabolite patterns carcinogen‐DNA adducts are generally qualitatively similar among animal species, individuals within a tissues an individual. However, wide quantitative differences observed between out‐bred including humans. These interindividual amounts activities enzymes that important the magnitude (10‐to 150‐fold) to those pharmacogenetic...
Expression of CD44, a transmembrane hyaluronan-binding glycoprotein, is variably considered to have prognostic significance for different cancers, including oral squamous cell carcinoma. Although unclear at present, tissue-specific expression particular isoforms CD44 might underlie the outcomes in currently available studies. We mined public transcriptomics databases gene data on and analyzed normal, immortalized tumour-derived human lines splice variants both transcript protein levels....
The toxicity of formaldehyde, a monomer released from certain polymeric dental materials, was studied in cultured human oral fibroblasts and epithelial cells. influences growth conditions were evaluated for both cell types, as well the role internal external thiol states. A one-hour exposure to formaldehyde decreased colony-forming efficiency (CFE) types concentration-dependent manner, although varied up 100-fold with conditions. Clearly, presence serum cysteine counteracted fibroblasts....
Toxicity is a complex phenomenon involving the potential adverse effect on range of biological functions. Predicting toxicity involves using combination experimental data (endpoints) and computational methods to generate set predictive models. Such models rely strongly being able integrate information from many sources. The required integration chemical sources requires, however, common language express our knowledge ontologically, interoperating services build reliable toxicology...