A5030030335- CRISPR and Genetic Engineering
- Gut microbiota and health
- Phytoestrogen effects and research
- Liver Disease Diagnosis and Treatment
- Endoplasmic Reticulum Stress and Disease
- Advanced biosensing and bioanalysis techniques
- Microbial Community Ecology and Physiology
- Environmental DNA in Biodiversity Studies
- Genomics and Phylogenetic Studies
- Lipid metabolism and biosynthesis
- Asthma and respiratory diseases
- Pluripotent Stem Cells Research
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- Pediatric health and respiratory diseases
- Digestive system and related health
- Gene Regulatory Network Analysis
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Clostridium difficile and Clostridium perfringens research
- Tryptophan and brain disorders
Discovery Institute
2017-2022
University of Pittsburgh
2017-2022
University of Georgia
2016-2019
Next-generation sequencing (NGS) of amplicons is used in a wide variety contexts. In many cases, NGS amplicon remains overly expensive and inflexible, with library preparation strategies relying upon the fusion locus-specific primers to full-length adapter sequences single identifying sequence or ligating adapters onto PCR products. Adapterama I, we presented universal stubs produce thousands unique index combinations modifiable system for incorporating them into Illumina libraries. Here,...
Abstract Next-generation sequencing (NGS) of amplicons is used in a wide variety contexts. Most NGS amplicon remains overly expensive and inflexible, with library preparation strategies relying upon the fusion locus-specific primers to full-length adapter sequences single identifying sequence or ligating adapters onto PCR products. In Adapterama I , we presented universal stubs produce thousands unique index combinations modifiable system for incorporating them into Illumina libraries. Here,...
Non-alcoholic fatty liver disease (NAFLD) has a high global prevalence with heterogeneous and complex pathophysiology that presents barriers to traditional targeted therapeutic approaches. We describe an integrated quantitative systems pharmacology (QSP) platform comprehensively unbiasedly defines states, in contrast just individual genes or pathways, promote NAFLD progression. The QSP can be used predict drugs normalize these states experimentally test predictions human acinus...
Abstract Clustered regularly interspaced short palindromic repeats (CRISPR)-based gene editing techniques find applications in many fields, such as molecular biology, cancer and disease modeling. In contrast to the knock-out procedure, a key step of CRISPR knock-in experiments is homology-directed repair process that requires donor constructs templates. Therefore, it desirable generate series templates efficiently cost-effectively. this study, we developed new strategy combines (i) Gibson...
ABSTRACT CRISPR-based gene knock-in at endogenous sites is desirable in multiple fields such as quantitative studies of signal transduction pathways and regulation, synthetic biology, disease modeling. Contrasting the knock-out procedure, a key step CRISPR procedure relies on homology-directed repairing (HDR) process that requires donor construct repair template. Therefore, it to generate series DNA constructs efficiently cost-effectively. In this study, we developed general Gibson assembly...