Ileana Tossolini

ORCID: 0000-0003-2274-2526
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About
Contact & Profiles
Research Areas
  • Viral Infectious Diseases and Gene Expression in Insects
  • Protein purification and stability
  • Virus-based gene therapy research
  • Chromosomal and Genetic Variations
  • Plant Molecular Biology Research
  • Genomics and Chromatin Dynamics
  • Yeasts and Rust Fungi Studies
  • Higher Education Teaching and Evaluation
  • E-Learning and Knowledge Management
  • CRISPR and Genetic Engineering
  • Viral gastroenteritis research and epidemiology
  • Biopolymer Synthesis and Applications
  • Plant nutrient uptake and metabolism
  • Educational Technology in Learning
  • RNA and protein synthesis mechanisms
  • Berry genetics and cultivation research
  • Light effects on plants
  • Bioenergy crop production and management

Universidad Nacional de Entre Ríos
2025

Instituto de Agrobiotecnología del Litoral
2021-2025

National University of the Littoral
2021-2025

Consejo Nacional de Investigaciones Científicas y Técnicas
2018-2025

In most organisms, the maturation of nascent RNAs is coupled to transcription. Unlike in animals, RNA polymerase II (RNAPII) transcribes microRNA genes (MIRNAs) as long and structurally variable pri-miRNAs plants. Current evidence suggests that miRNA biogenesis complex assembly initiates early during transcription However, it unknown whether processing occurs co-transcriptionally. Here, we used native elongating transcript sequencing data imaging techniques demonstrate plant We found entire...

10.1038/s41477-022-01125-x article EN cc-by Nature Plants 2022-04-21

Transposable elements (TEs) constitute a major portion of plant genomes and play key roles in shaping genome architecture, regulating gene expression, driving evolution. In this study, we generated comprehensive curated TE library for the woodland strawberry (Fragaria vesca) by integrating two bioinformatic pipelines (EDTA DeepTE). Our annotation revealed that TEs account approximately 37% F. vesca genome. Analysis TE-derived inverted repeats (IRs) miniature inverted-repeat transposable...

10.1101/2025.03.20.644342 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2025-03-20

El objetivo de este artículo es presentar el análisis experiencias innovadoras en ocho asignaturas carreras grado la Universidad Nacional Entre Ríos. Estas se consolidaron durante pandemia y continúan actual contexto signado por los crecientes cambios tecnológicos socioculturales educación superior. Las seleccionadas postulan una ruptura con las prácticas pedagógicas establecidas a partir integración tecnologías digitales e impulsan modelos mixtos enseñanza participación estudiantes. Se...

10.21556/edutec.2025.91.3589 article ES cc-by Edutec Revista Electrónica de Tecnología Educativa 2025-03-27

<title>Abstract</title> Transposable elements (TEs) constitute a major portion of plant genomes and play key roles in shaping genome architecture, regulating gene expression, driving evolution. In this study, we generated comprehensive curated TE library for the woodland strawberry (<italic>Fragaria vesca</italic>) by integrating two bioinformatic pipelines (EDTA DeepTE). Our annotation revealed that TEs account approximately 37% <italic>F. vesca</italic> genome. Analysis TE-derived inverted...

10.21203/rs.3.rs-6266511/v1 preprint EN Research Square (Research Square) 2025-04-22

For the production of recombinant protein therapeutics in mammalian cells, a high rate gene expression is desired and hence strong viral-derived promoters are commonly used. However, they usually induce cellular stress can be susceptible to epigenetic silencing. Endogenous promoters, which coordinates their activity with bioprocess dynamics while at same time maintain levels, may help avoid such drawbacks. In this work, endogenous were identified based on levels RNA-seq data CHO-K1 cells...

10.22541/au.170668679.92685341/v1 preprint EN Authorea (Authorea) 2024-01-31

Summary In most organisms, the maturation of nascent RNAs is coupled to transcription, undergoing many processing steps co-transcriptionally. Unlike in animals, RNA polymerase II (RNAPII) transcribes microRNAs (miRNAs) as long and structurally variable pri-miRNAs plants. Current evidence suggests that miRNA biogenesis complex assembly initiates early during transcription However, it unknown whether occurs Here, we show plant a process relies on formation DNA:RNA hybrids (R-loops) between...

10.1101/2021.09.07.459282 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2021-09-07

Abstract For the production of recombinant protein therapeutics in mammalian cells, a high rate gene expression is desired and hence strong viral-derived promoters are commonly used. However, they usually induce cellular stress can be susceptible to epigenetic silencing. Endogenous promoters, which coordinates their activity with bioprocess dynamics while at same time maintain levels, may help avoid such drawbacks. In this work, endogenous were identified based on levels RNA-seq data CHO-K1...

10.1101/2021.10.13.464282 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2021-10-14
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