A5075252986- RNA modifications and cancer
- RNA Research and Splicing
- Cardiomyopathy and Myosin Studies
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- Trypanosoma species research and implications
- Biochemical and Molecular Research
Bar-Ilan University
2020-2025
Goodman (Japan)
2020
The parasite Trypanosoma brucei causes African sleeping sickness that is fatal to patients if untreated. Parasite differentiation from a replicative slender form into quiescent stumpy promotes host survival and transmission. Long noncoding RNAs (lncRNAs) are known regulate cell in other eukaryotes. To determine whether lncRNAs also involved differentiation, we used RNA sequencing survey the T. genome, identifying 1428 previously uncharacterized lncRNA genes. We find grumpy key regulator...
The protozoan parasite Trypanosoma brucei undergoes a complex life cycle, moving between its mammalian host and the blood-feeding tsetse fly vector. two major surface proteins expressed by procyclic forms in insect midgut, EP GPEET procyclin, are transcribed from polycistronic transcription unit RNA polymerase I. Here we identify long non-coding RNA, TblncRNA-23, that is encoded procyclin genes. TblncRNA-23 localizes to nucleolus also associates with polysomes. Overexpression of down...
The parasite Trypanosoma brucei cycles between an insect and a mammalian host is the causative agent of sleeping sickness. Here, we performed high-throughput mapping pseudouridines (Ψs) on mRNA from two life stages parasite. analysis revealed ~273 Ψs, including developmentally regulated Ψs that are guided by homologs pseudouridine synthases (PUS1, 3, 5, 7). Mutating U undergoes pseudouridylation in 3' UTR valyl-tRNA synthetase destabilized level. To investigate mechanism which Ψ affects...
The parasite Trypanosoma brucei is the causative agent of sleeping sickness and cycles between insect mammalian hosts. appears to lack conventional transcriptional regulation protein coding genes, mRNAs are processed from polycistronic transcripts by concerted action trans-splicing polyadenylation. Regulation mRNA function mediated mainly RNA binding proteins affecting stability translation. In this study, we describe identification 62 non-coding (nc) RNAs that developmentally regulated...
The parasite Trypanosoma brucei is the causative agent of sleeping sickness, and cycles between insect mammalian hosts. appears to lack conventional transcriptional regulation protein coding genes, mRNAs are processed from polycistronic transcripts by concerted action trans-splicing polyadenylation. Regulation mRNA function mediated mainly RNA binding proteins (RBPs) affecting stability translation. In this study, we describe identification 62 non-coding (nc) RNAs that developmentally...