Most of the cells possess complement regulatory proteins (CRPs) that protect them against complement-mediated damage. In our previous work we revealed human articular chondrocytes express CPRs. Moreover, increase CRPs expression after treatment with proinflammatory cytokines IL-1beta and TNF-alpha has been demonstrated by ELISA technique. Chondroprotective cytokine IL-4 stimulated CD46 only. this RT-PCR technique was used to evaluate mRNA cell surface CPRs in cultured isolated IL-1beta,...

The effect of short-term immunosuppressive treatment with antithymocyte serum-procarbazine (ATS-PCH) and cyclosporin-A (Cy-A) on survival allogeneic rib chondrocyte grafts was examined morphologically by evaluation specific humoral cellular antigraft immunity. latter were evaluated means leukoagglutination the indirect migration inhibition assay, respectively. Untreated recipients syngeneic chondrocytes untreated whole cartilage served as controls. Transplanted syngenic formed cartilaginous...

Rat chondrocytes transplanted intramuscularly in rabbits produced cartilage. In 1-day-old transplants remained viable. After 1 week peripheral of the transplant were dead and cartilage was surrounded resorbed by macrophages. 2-week-old deteriorated invaded fibroblast-like cells Sera that received two or three consecutive rat with 2-week intervals contained high titer antichondrocyte cytotoxic antibodies. A part activity could be removed absorption splenocytes. Western blot analysis lysates...

Articular cartilage and the synovial membrane both ensure smooth action of joints; however, influence chondrocytes on metabolism remains unclear. The secretory activity is usually studied in cell cultures may differ from that intact cartilage. According to McCutchen's theory 'weeping' joint lubrication, loading articular during motion squeezes fluid with lubricating properties purpose study was obtain interstitial (CIF) evaluate its gene expression cells. CIF rinsed out newborn rats at a...

Endometriosis is a common gynecological disorder defined as the presence of endometrial-like tissue (glands and stroma) outside uterus. The etiopathogenesis endometriosis still poorly recognized. It speculated that stage-specific embryonic antigen 1 (SSEA-1)-positive stem-like glandular epithelial cells may contribute to development disease. synthesis SSEA-1 mediated by fucosyltransferase 4 encoded FUT4 gene. Therefore, this study aimed evaluate specific expression mRNA in biopsies...

Syngeneic rat chondrocytes isolated from the articular-epiphyseal cartilage complex were suspended in hyaluronic acid and transplanted intramuscularly or into joint surface defects. Transplants fixed ruthenium hexammonium trichloride embedded glycol methacrylate. In nodules produced intramuscularly, chondrocyte hypertrophy matrix calcification observed after 2 wk. Partial ossification occurred 4 wk was almost completely replaced by an ossicle 8 Only small, dispersed groups of remained within...

In endochondral ossification septoclasts and osteoclasts (also called chondroclasts) release growth factors deposited in non-calcified calcified zones of the plate. They stimulate, within metaphysis, initial stages bone formation. We have recently reported quantitation several cartilage from calf costochondral junction. Data analogous human could possibly help to choose efficient combinations for clinical applications, but amount needed analysis numerous would be difficult collect. The...

Rabbit serum produced after transplantation of isolated rat chondrocytes [sensitized rabbit (SRS)] demonstrated M r ~ 74- and ~23-kDa (western blot analysis) antigens in chondrocyte extracts. Only the latter remained reduction 2-mercaptoethanol. Protein sequence analysis 23-kDa chondrocyte-associated antigen (CAA) revealed that it corresponds to transmembrane Tmp21 protein belonging p24 family. These proteins mainly participate traffic between endoplasmic reticulum Golgi complex some cells...

Rat syngeneic and allogeneic chondrocytes were transplanted intramuscularly or into defects prepared in articular cartilage (intracartilaginous transplants). Recipients of transplants received cyclosporin A (CsA), cladribine (2-chlorodeoxyadenosine, 2-CdA), both drugs combination. Transplants taken for examination after 5 weeks. Cartilage formed by was ossified. Allogeneic resorbed infiltrating cells. CsA 2-CdA partially suppressed, these agents combination strongly formation infiltrations....

Endochondral bone formation is orchestrated by growth factors produced chondrocytes and deposited in the cartilage matrix. Whilst some of these have been identified, complete list their relationship remains unknown. In present study, were isolated from non‑calcified calcified costochondral junctions. Cartilage dissected ribs 6‑20‑week‑old calves was purchased a local butcher within 24 h death animal. The isolation involved hyaluronidase digestion, guanidinium hydrochloride (GuHCl)...

Rat chondrocytes isolated from the articular-epiphyseal cartilage complex were transplanted into defects prepared in articular and subchondral bone. Transplants taken for examination after 3 8 wk. Cartilage formed by syngeneic did not evoke formation of infiltrations. Contrary to that, vicinity produced allogeneic numerous infiltrating cells present resorption could be observed. Cyclosporine-A (CsA) treatment recipients only partially suppressed accumulation matrix resorption....

Rat chondrocytes isolated from the articular–epiphyseal cartilage complex were transplanted into defects prepared in articular and subchondral bone. Transplants taken for examination after 3 8 wk. Cartilage formed by syngeneic did not evoke formation of infiltrations. Contrary to that, vicinity produced allogeneic numerous infiltrating cells present resorption could be observed. Cyclosporine-A (CsA) treatment recipients only partially suppressed accumulation matrix resorption....