A5074595723- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Environmental DNA in Biodiversity Studies
- Bacteriophages and microbial interactions
- Protist diversity and phylogeny
- Polar Research and Ecology
- Gut microbiota and health
- Genetics, Bioinformatics, and Biomedical Research
- SARS-CoV-2 detection and testing
- Methane Hydrates and Related Phenomena
- Biosensors and Analytical Detection
Universidade do Porto
2020-2025
Institute for Biotechnology and Bioengineering
2020
University of Lisbon
2020
Abstract Background High-throughput sequencing of the full-length 16S rRNA gene has improved taxonomic classification prokaryotes found in natural environments. However, shorter regions from same gene, like V4-V5 region, can provide more cost-effective high throughput. It is unclear which approach best describes prokaryotic communities underexplored In this study, we hypothesize that high-throughput combined with adequate databases improves description environments comparison a short region...
Bacterial natural products (NPs) are still a major source of new drug leads. Polyketides (PKs) and non-ribosomal peptides (NRP) two pharmaceutically important families NPs recent studies have revealed Antarctica to harbor endemic polyketide synthase (PKS) peptide synthetase (NRPS) genes, likely be involved in the production novel metabolites. Despite this, diversity secondary metabolites genes is poorly explored. In this study, computational bioprospection approach was employed study...
Research on marine microbial communities is growing, but studies are hard to compare because of variation in seawater sampling protocols. To help researchers the inter-comparison that use different methodologies, as well them design future campaigns, we developed EuroMarine Open Science Exploration initiative (EMOSE). Within EMOSE framework, sampled thousands liters from a single station NW Mediterranean Sea (Service d'Observation du Laboratoire Arago [SOLA], Banyuls-sur-Mer), during one...
The monitoring of cities' wastewaters for the detection potentially pathogenic viruses and bacteria has been considered a priority during COVID-19 pandemic to monitor public health in urban environments. methodological approaches frequently used this purpose include deoxyribonucleic acid (DNA)/Ribonucleic (RNA) isolation followed by quantitative polymerase chain reaction (qPCR) reverse transcription (RT)‒qPCR targeting genes. More recently, application metatranscriptomic opened opportunities...
Antarctic deserts, such as the McMurdo Dry Valleys (MDV), represent extremely cold and dry environments. Consequently, MDV are suitable for studying environment limits on cycling of key elements that necessary life, like nitrogen. The spatial distribution biogeochemical drivers nitrogen-cycling pathways remain elusive in deserts because most studies focus specific genes and/or organisms. In this study, we analyzed metagenome relevant environmental data 32 soils to generate a complete picture...