A5061701164- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurotransmitter Receptor Influence on Behavior
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Receptor Mechanisms and Signaling
- Neuroendocrine regulation and behavior
- Memory and Neural Mechanisms
- Pharmacological Effects of Natural Compounds
- Anesthesia and Sedative Agents
- Neurobiology and Insect Physiology Research
- Ion channel regulation and function
- Image Processing Techniques and Applications
- Single-cell and spatial transcriptomics
University of Washington
2021-2024
University of California, San Francisco
2020-2023
Washington Center
2023
Peripheral nerve injury-induced neuropathic pain is a chronic and debilitating condition characterized by mechanical hypersensitivity. We previously identified microglial activation via release of colony-stimulating factor 1 (CSF1) from injured sensory neurons as mechanism contributing to pain. Here, we show that intrathecal administration CSF1, even in the absence injury, sufficient induce behavior, but only male mice. Transcriptional profiling morphologic analyses after CSF1 showed robust...
Repeated pairing of a drug with neutral stimulus, such as cue or context, leads to the attribution drug's reinforcing properties that and exposure stimulus in absence can elicit drug-seeking. A principal role for NAc response drug-associated stimuli has been well documented. Direct indirect pathway medium spiny neurons (dMSNs iMSNs) have shown bidirectionally regulate cue-induced heroin-seeking rats expressing addiction-like phenotypes, shift activity toward direct mice following cocaine...
Primary sensory neurons are generally considered the only source of dorsal horn calcitonin gene-related peptide (CGRP), a neuropeptide critical to transmission pain messages. Using tamoxifen-inducible Calca CreER transgenic mouse, here we identified distinct population CGRP-expressing excitatory interneurons in lamina III spinal cord and trigeminal nucleus caudalis. These have spine-laden, dorsally directed, dendrites, ventrally directed axons. As under resting conditions, CGRP tonic...
High-throughput volumetric fluorescent microscopy pipelines can spatially integrate whole-brain structure and function at the foundational level of single cells. However, conventional protein (FP) modifications used to discriminate cells possess limited efficacy or are detrimental cellular health. Here, we introduce a synthetic nondeleterious nuclear localization signal (NLS) tag strategy, called “Arginine-rich NLS” (ArgiNLS), that optimizes genetic labeling downstream image segmentation by...
Abstract The general consensus is that increases in neuronal activity the anterior cingulate cortex (ACC) contribute to pain’s negative affect. Here, using vivo imaging of calcium dynamics mice, we report nitrous oxide, a anesthetic reduces pain affect, paradoxically, ACC spontaneous activity. As expected, noxious stimulus also increased However, as oxide baseline activity, relative change from pre-stimulus was significantly less than absence anesthetic. We suggest this represents neural...
Calcium binding proteins are expressed throughout the central and peripheral nervous system disruption of their activity has major consequences in a wide array cellular processes, including transmission nociceptive signals that processed at level spinal cord. We previously reported calcium protein, hippocalcin-like 4 (Hpcal4), is heavily interneurons superficial dorsal horn, its expression significantly downregulated TR4 mutant mouse model exhibits pain itch deficits due to loss...
High-throughput volumetric fluorescent microscopy pipelines can spatially integrate whole-brain structure and function at the foundational level of single-cells. However, conventional protein (FP) modifications used to discriminate single-cells possess limited efficacy or are detrimental cellular health. Here, we introduce a synthetic non-deleterious nuclear localization signal (NLS) tag strategy, called 'Arginine-rich NLS' (ArgiNLS), that optimizes genetic labeling downstream image...
ABSTRACT Initial drug use promotes the development of conditioned reinforcement, whereby reinforcing properties a become attributed to drug-associated stimuli, such as cues and contexts. A principal role for nucleus accumbens (NAc) in response stimuli has been well-documented. In particular, direct indirect pathway medium spiny neurons (dMSNs iMSNs) have shown bidirectionally regulate cue-induced heroin-seeking rats expressing addiction-like phenotypes, shift NAc activity towards mice...