A5050356164- Extracellular vesicles in disease
- Nanoparticle-Based Drug Delivery
- Systemic Lupus Erythematosus Research
- RNA modifications and cancer
- Atherosclerosis and Cardiovascular Diseases
- Computational Drug Discovery Methods
- Lipoproteins and Cardiovascular Health
- Enzyme Structure and Function
- Inhalation and Respiratory Drug Delivery
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Nanopore and Nanochannel Transport Studies
- Characterization and Applications of Magnetic Nanoparticles
- RNA Research and Splicing
- Innovative Microfluidic and Catalytic Techniques Innovation
- Gold and Silver Nanoparticles Synthesis and Applications
ETH Zurich
2022-2025
Abstract The mechanisms that underlie the regulation of enzymatic reactions by biomolecular condensates and how they scale with compartment size remain poorly understood. Here we use intrinsically disordered domains as building blocks to generate programmable NADH-oxidase (NOX) different sizes spanning from nanometers microns. These domains, derived three distinct RNA-binding proteins, each possessing net charge, result in formation characterized a comparable high local concentration enzyme...
Mesoscale interactions—such as biomolecular coronas, transient associations, aggregation, and fusion—are increasingly recognized for their biological significance and, under certain conditions, are essential extracellular nanoparticles to fulfill functions. Among these interactions, the binding between vesicles lipoproteins has recently gained attention its potential impact on vesicle function fate in vivo. These interactions must be understood clarify possibly engineer exploit modes of...
Extracellular vesicles (EVs) hold promise for liquid biopsy and drug delivery applications. However, their heterogeneous nature poses challenges efficient selective isolation from complex biofluids. Here, an method based on phase-separated zwitterionic (ZW) coacervates is developed. These form over a wide range of pH values ionic strengths, ensuring compatibility with all They exhibit antifouling properties that minimize nonspecific binding, allowing the EVs biofluids upon functionalization...
The calculation of relative free-energy differences between different compounds plays an important role in drug design to identify potent binders for a given protein target. Most rigorous methods based on molecular dynamics simulations estimate the difference pairs ligands. Thus, comparison multiple ligands requires construction "state graph", which are connected by alchemical transformations. computational cost can be optimized reducing state graph minimal set However, this may require...
Extracellular vesicles (EVs) are membrane-enclosed biological nanoparticles with potential as diagnostic markers and carriers for therapeutics. Characterization of EVs poses severe challenges due to their complex structure composition, requiring the combination orthogonal analytical techniques. Here, we demonstrate how liquid chromatography combined multi-angle light scattering (MALS) fluorescence detection in one single apparatus can provide multiparametric characterization EV samples,...
Coating synthetic nanoparticles (NPs) with lipid membranes is a promising approach to enhance the performance of nanomaterials in various biological applications, including therapeutic delivery target organs. Current methods for achieving this coating often rely on bulk approaches which can result low efficiency and poor reproducibility. Continuous processes coupled quality control represent an attractive strategy manufacture products consistent attributes high yields. Here, concept...
Extracellular vesicles (EVs) function as communicators between cells in the extra- cellular space. However, extravesicular space is rich biomolecules, which form a layer on surfaces. In this work, we investigated how identity of naive EVs changes with deposition albumin layer, known commonly corona. The heterogeneity was reflected also their At low concentrations albumin, corona may change apparent zeta potential EVs. cell culture (≈ 60μM), surface markers become unavailabile for antibodies...
Extracellular vesicles (EVs) are membrane enclosed biological nanoparticles with potential as diagnostic markers and carriers for therapeutics. Characterization of EVs poses severe challenges due to their complex structure composition, requiring the combination orthogonal analytical techniques. Here, we demonstrate how liquid chromatography combined multi-angle light scattering (MALS) fluorescence detection can provide multi-parametric characterization EV samples, including concentration...