A5100671450- RNA Research and Splicing
- RNA modifications and cancer
- Reproductive Biology and Fertility
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Ovarian function and disorders
- Cancer-related gene regulation
- RNA Interference and Gene Delivery
- Reproductive System and Pregnancy
- Thin-Film Transistor Technologies
- Pluripotent Stem Cells Research
- Cancer, Hypoxia, and Metabolism
- Prenatal Screening and Diagnostics
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- Nanowire Synthesis and Applications
- Circular RNAs in diseases
- Reproductive Health and Technologies
- Genomics and Chromatin Dynamics
- Diet and metabolism studies
- Hemophilia Treatment and Research
- Monoclonal and Polyclonal Antibodies Research
- Glutathione Transferases and Polymorphisms
- Fetal and Pediatric Neurological Disorders
- Hepatitis Viruses Studies and Epidemiology
Chinese Academy of Sciences
2010-2025
Institute of Genetics and Developmental Biology
2019-2025
Shanghai Jiao Tong University
2025
University of Illinois Urbana-Champaign
2024-2025
Northeast Forestry University
2022-2024
Defence Electronics Research Laboratory
2023
China University of Mining and Technology
2023
Affiliated Hospital of Qingdao University
2020-2022
Qingdao University
2020-2022
Southeast University
2022
Abstract Poly(A)-tail-mediated post-transcriptional regulation of maternal mRNAs is vital in the oocyte-to-embryo transition (OET). Nothing known about poly(A) tail dynamics during human OET. Here, we show that length and internal non-A residues are highly dynamic OET, using poly(A)-inclusive RNA isoform sequencing (PAIso-seq). Unexpectedly, undergo global remodeling: after deadenylation or partial degradation into 3ʹ-UTRs, they re-polyadenylated to produce polyadenylated intermediates,...
Abstract Endometriosis, a pathological condition in which the endometrium grows outside uterus, is one of most common causes female infertility; it diagnosed 25–40% infertile women. The mechanism by endometriosis affects fertility females remains largely unknown. We examined ultrastructure oocytes from patients with minimal or mild and control undergoing vitro fertilization (IVF) treatment transmission electron microscopy (TEM) to investigate physiological significance oocyte quality for...
Abstract Message RNA poly(A) tails are vital for their function and regulation. However, the full-length sequence of mRNA isoforms with remains undetermined. Here, we develop a method at single-cell level sensitivity that enables quantification along cDNA while reading non-adenosine residues within precisely, which name inclusive isoform sequencing (PAIso−seq). Using this method, can quantify specific tail length. More interestingly, find 17% mRNAs harbor non-A body in mouse GV oocytes. We...
MicroRNA-128 (miR-128) is an attractive therapeutic molecule with powerful glioblastoma regulation properties.
Mammalian early embryos maintain accurate genome integrity for proper development within a programmed timeline despite constant assaults on their DNA by replication, demethylation and genetic defects transmitted from germ cells. However, how is safeguarded during mammalian embryonic remains unclear. BCAS2 (Breast Carcinoma Amplified Sequence 2), core component of the PRP19 complex involved in pre-mRNA splicing, plays an important role damage response through RPA complex, key regulator...
Do PATL2 mutations account for female infertility with oocyte germinal vesicle (GV) arrest?Four of nine independent families GV arrest were identified biallelic mutations, suggesting that these may be responsible maturation in primary infertile women.Recently, two studies have demonstrated some women at the stage was caused by PATL2. encodes protein PAT1 homolog 2, an RNA-binding act as a translational repressor.In this study, unrelated females presenting recruited during treatment early...
The heterogeneity of CARM1 controls first cell fate bias during early mouse development. However, how this is established unknown. Here, we show that Carm1 mRNA a variety specific exon-skipping splicing (ESS) isoforms in two-cell to four-cell embryos contribute heterogeneity. Disruption paraspeckles promotes the ESS precursor mRNAs (pre-mRNAs). LincGET, but not Neat1, required for paraspeckle assembly and inhibits pre-mRNAs embryos. We further find LincGET recruits gene locus through HNRNPU....
Background Brain-derived neurotropic factor (BDNF) was originally described in the nervous system but has been shown to be expressed ovary tissues recently, acting as a paracrine/autocrine regulator required for developments of follicles and oocytes. Although it is generally accepted that chronic stress impairs female reproduction decreases expression BDNF limbic structures central system, which contributes mood disorder. However, not known whether affects oocytes developments, nor ovary....
The morphological assessment of oocytes is important for embryologists to identify and select MII in IVF/ICSI cycles. Dysmorphism decreases viability the developmental potential as well clinical pregnancy rate. Several reports have suggested that with a dark zona pellucida (DZP) correlate outcome IVF treatment. However, effect DZP on oocyte quality, fertilization, implantation, were not investigated detail. In this study, retrospective analysis was performed 268 infertile patients fallopian...
Abstract Oocyte in vitro maturation is a technique assisted reproductive technology. Thousands of genes show abnormally high expression maturated metaphase II (MII) oocytes compared to those matured vivo bovines, mice, and humans. The mechanisms underlying this phenomenon are poorly understood. Here, we use poly(A) inclusive RNA isoform sequencing (PAIso-seq) for profiling the transcriptome-wide tails both mouse human oocytes. Our results demonstrate that observed increase maternal mRNA...
Platelets play a critical role in hemostasis at sites of injury and are capable interacting with various types cells the bloodstream. The promise utilizing platelets for diagnostic therapeutic applications has motivated development facile strategies to functionalize platelets. However, small size, lack nucleus efficient protein machinery, low tolerance chemicals transfection agents have posed significant challenges chemical or genetic engineering. Here, first time, we report successful...
Phase behaviors of the liquids in nanospaces are particular interest to understand thermodynamics liquid on nanoscale and for their applications that involve confined systems. However, many cases, inconsistent observations melting point variation often revealed by different groups. Ionic a special kind liquid. Here, using merits nonvolatile nature ionic liquids, we realized encapsulation inside mesopores silica oxide nanoparticles with complete removal compressed gas under high-vacuum...
Chondroitin sulfate-g-poly(ε-caprolactone) (CP) copolymers were synthesized via atom transfer radical addition (ATRA). The CP self-assembled into micelles in water, and the could be used to encapsulate a hydrophobic anticancer drug, camptothecin (CPT), core for tumor targeting delivery. physicochemical properties of CPT-loaded thoroughly characterized. For vitro test, CPT release, protection lactone ring from hydrolysis cellular uptake studied. cell-killing apoptosis-inducing effects using...
Maslinic acid is an active member of pentacyclic triterpenes predominantly found in dietary plants including hawthorn berries and olive fruit skins. It has been reported to show immense pharmacological biological importance anticancer property. This research was initiated explore the potential maslinic against human neuroblastoma. The effects on cellular apoptosis, ROS generation, cell migration invasion, caspase activation targeting MAPK/ERK signaling pathway were investigated....
Abstract Transcription is silent during the mammalian oocyte-to-embryo transition (OET) until zygotic genome activation (ZGA). Therefore, OET relies on post-transcriptional regulation of maternal mRNA, among which poly(A) tail lengths have been found to regulate translation for a small number genes 1–3 . However, transcriptome-wide length dynamics and their role in gene expression remain unknown. Here, we quantified mRNA using PAIso-seq1 PAIso-seq2 4,5 , two methods with different underlying...
BACKGROUNDBefore human MII oocytes are vitrified they usually denuded from their cumulus cells. In this study we wanted to investigate the effects of an intact corona radiata on vitrification and fertilization oocytes.