A5102726633- Immune Cell Function and Interaction
- Inflammatory Bowel Disease
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Eosinophilic Esophagitis
- Genetic factors in colorectal cancer
- Escherichia coli research studies
- Renal and related cancers
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Zebrafish Biomedical Research Applications
- Microscopic Colitis
- Pancreatic function and diabetes
- Cleft Lip and Palate Research
- Craniofacial Disorders and Treatments
- Congenital heart defects research
- Digestive system and related health
- Congenital Anomalies and Fetal Surgery
- Developmental Biology and Gene Regulation
Tokai University
2015-2023
Group 2 innate lymphoid cells (ILC2s) are derived from common progenitors (CLPs) via several specific precursors, and the transcription factors essential for ILC2 differentiation have been extensively studied. However, external regulating commitment to ILC lineage as well sites stromal that constitute optimal microenvironment ILC2-specific not fully defined. In this study, we demonstrate three key factors, concentration of interleukin 7 (IL-7) strength duration Notch signaling, coordinately...
Intestinal fibrosis is a major complication of Crohn's disease (CD). The profibrotic protein transforming growth factor-β (TGF-β) has been considered to be critical for the induction fibrotic program. TGF-β ability induce not only expression extracellular matrix (ECM) including collagen, but also production plasminogen activator inhibitor-1 (PAI-1) that prevents enzymatic degradation ECM during onset diseases. However, significance PAI-1 in developing intestinal fully understood. In present...
Notch signaling has been shown to contribute murine pancreatic development at various stages. Delta-like 1 (Dll1) or Jagged1 (Jag1) are the ligands that solely function trigger this during bud stage (~e9.5) after birth, respectively. However, it not elucidated whether these required later (e10.5-18.5) when particular pancreas structures form. Here, we detected dual expression of Dll1 and Jag1 in epithelium e10.5, which was restricted ductal cell lineage, including centroacinar cells...
Background and aim Adherent-invasive E. coli (AIEC) has been identified as a pathobiont associated with Crohn’s disease (CD), that prefers to grow in inflammatory conditions. Although the colonization by AIEC is implicated progression of exacerbates inflammation murine colitis models, recognition response host immunity remains elusive. Methods Antibiotic treated female C57BL/6 mice were inoculated commensal LF82 strains. Luminal-IgA fractions prepared from feces their binding other strains...
Delta-like 4 (Dll4)-mediated Notch signaling is critical for specifying T-cell fate, but how Dll4-mediated actually contributes to development in the thymus remains unclear. To explore this mechanism thymic three-dimensional structure, we performed fetal organ culture using Dll4-deficient mice. DN1a/b+DN2mt cells, which had not yet committed either αβ T or γδ T/NK cell lineage, did differentiate into lineage despite lack of fate conversion other lineages. However, DN3 cells efficiently...
T-cell development in the thymus is dependent on Notch signaling induced by interaction of Notch1, present immigrant cells, with a ligand, delta-like (Dll) 4, thymic epithelial cells. Phylogenetic analysis characterizing properties Dll4 molecule suggests that emerged from common ancestor lobe- and ray-finned fishes diverged into bony terrestrial organisms, including mammals. The evolved cartilaginous before Dll4, suggesting Dll1 instead Dll4. In this study, we compared function both Dll...
The intracellular fragment of Notch1, a mediator Notch signaling that is frequently detected in thymic immigrants, critical for specifying T-cell fate the thymus, where Delta-like 4 (Dll4) functions as ligand on epithelium. However, such has not been mature T cells, how contributes to their response secondary lymphoid organs yet fully defined. Here, we marked expression Dll4 stromal cells and active Notch1 (Notch1 domain, N1ICD) CD4+ follicles Peyer's patches (PPs). In addition,...