A5025176430- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Tuberculosis
- Traditional Chinese Medicine Studies
- Pneumonia and Respiratory Infections
- Genomics and Phylogenetic Studies
- Diagnosis and treatment of tuberculosis
- HIV/AIDS oral health manifestations
- Dysphagia Assessment and Management
- Respiratory viral infections research
- RNA modifications and cancer
- Pneumocystis jirovecii pneumonia detection and treatment
- Molecular Biology Techniques and Applications
University of Washington
2018-2025
Seattle University
2023
Tongue dorsum swabs have shown promise as alternatives to sputum for detecting Mycobacterium tuberculosis (MTB) in patients with pulmonary (TB). Some of the most encouraging results come from studies that used manual quantitative PCR (qPCR) analyze swabs. Studies using automated Cepheid Xpert MTB/RIF Ultra qPCR test (Xpert Ultra) exhibited less sensitivity tongue swabs, possibly because is optimized testing sputum, not swab samples. Using two new sample preprocessing methods demonstrated...
Tongue dorsum swabbing is a potential alternative to sputum collection for tuberculosis (TB) testing. Previous studies showed that Cepheid Xpert MTB/RIF Ultra (Xpert Ultra) can detect
Diagnostic tests for tuberculosis (TB) usually require collection of sputum, a viscous material derived from human airways. Sputum can be difficult and hazardous to collect challenging process in the laboratory.
Oral swab analysis (OSA) has been shown to detect Mycobacterium tuberculosis (MTB) DNA in patients with pulmonary (TB). In previous analyses, qPCR testing of samples collected from tongue dorsa was up 93% sensitive relative sputum GeneXpert, when 2 swabs per patient were tested. The present study modified sample collection methods increase biomass and characterized the viability bacilli swabs. A targeting conserved bacterial ribosomal rRNA gene (rDNA) sequences used quantify samples. There...
Abstract Microbiological diagnosis of pediatric pulmonary tuberculosis (TB) is challenging due to the difficulty collecting and testing sputum from children. We investigated whether easily-obtained oral swab samples are useful alternatives or supplements sputum. Oral swabs induced (IS) were collected 201 South African children with suspected TB. IS tested by mycobacterial culture Xpert MTB/RIF. PCR targeting IS6110. Children categorized as Confirmed TB (microbiologic confirmation on IS),...
Testing for mycobacterial lipoarabinomannan (LAM) in urine is a practical but insensitive alternative to sputum testing diagnose tuberculosis (TB) people with HIV (PWH). Here, we evaluated LAM alongside PCR-based tests Mycobacterium (MTB) DNA tongue swabs.
ABSTRACT Tongue swab (TS) sampling combined with quantitative PCR (qPCR) to detect Mycobacterium tuberculosis (MTB) DNA is a promising alternative sputum testing for (TB) diagnosis. In prior studies, the sensitivity of tongue swabbing has usually been lower than sputum. this study, we evaluated two strategies improve sensitivity. one, centrifugation was used concentrate dorsum bacteria from 2-mL suspensions eluted high-capacity foam samples. The pellets were resuspended as 500-µL...
Oral samples show promise for tuberculosis (TB) diagnosis. Data from different and people with sputum scarce TB are limited. We assessed Xpert MTB/RIF Ultra (Ultra) in symptomatic at clinics (Cohort A, n=891) or antiretroviral therapy (ART)-initiation without syndromic preselection B, n=258). In Cohort we collected oral washes (OWs) and, separately, tongue swabs (flocked, foam heat). OWs, three flocked (comparing one heat to two pooled swabs) buccal swabs, periodontal brushes. offered...
Tongue swabs (TS) are novel non-sputum specimens for molecular diagnosis of tuberculosis (TB). Analysis by using Cepheid Xpert MTB/RIF Ultra (Xpert Ultra) is less sensitive than the use certain manual qPCR methods but desirable given widespread and familiarity Ultra. This study evaluated an easy-to-use protocol analyzing TS samples from symptomatic South African patients were previously collected tested a that requires heating block (termed Heat+TE). Replicate, paired same newly reported...
Tongue swab (TS) sampling combined with qPCR to detect
This standard operating procedure (SOP) outlines the methodology for collection of tongue swabs subsequent testing tuberculosis molecular assays that require a dry swab as input material.
Tongue swabs hold promise as a non-invasive sample for diagnosing tuberculosis (TB). However, their utility replacements sputum has been limited by varied diagnostic performance in PCR assays compared to sputum. The use of silica-based DNA extraction methods may limit sensitivity due incomplete lysis Mycobacterium (MTB) cells and co-extraction non-target nucleic acid, which inhibit PCR. Specificity also be compromised because these are labor-intensive prone cross-contamination. To address...
Abstract Testing for mycobacterial lipoarabinomannan (LAM) in urine is a practical but insensitive alternative to sputum testing diagnose tuberculosis (TB) people with HIV (PWH). We evaluated LAM conducted parallel tests Mycobacterium DNA oral swabs. In cohort of 131 South Africans (92% HIV), combined and swab was significantly more sensitive than either sample tested alone (57% vs. 35% 39%, respectively), 97% specific, compared reference (TB culture Xpert Ultra). Complementary non-sputum...
ABSTRACT Tongue dorsum swabbing is a potential alternative to sputum collection for tuberculosis (TB) testing. Previous studies showed that Cepheid Xpert® MTB/RIF Ultra (Xpert Ultra) can detect Mycobacterium (MTB) DNA in tongue swabs stored buffer, with 72% sensitivity and 100% specificity relative microbiological reference standard (sputum MRS). The present study evaluated more convenient sample protocol (dry swab storage), combined streamlined processing protocols, side-by-side analysis...
Abstract The gut microbiome is recognized as a critical regulator of human diseases. Constituents the microbiota and their individual activities can affect broad range disease states related to autoimmunity, cancer, infection, metabolism, mental health, toxicant exposure. A substantial number species are not culturable, limiting study in vitro. Sequencing methods have allowed quantification composition microbiome, but characterize physiological status bacterial remain limited. Ribosomal RNA...