A5101667401- Tuberculosis Research and Epidemiology
- Psoriasis: Treatment and Pathogenesis
- Immune Cell Function and Interaction
- Immune responses and vaccinations
- Arabic Language Education Studies
- Education and Islamic Studies
- Second Language Acquisition and Learning
- Genetics and Neurodevelopmental Disorders
- HIV Research and Treatment
- Biochemical and Molecular Research
- Whipple's Disease and Interleukins
- RNA modifications and cancer
- Film in Education and Therapy
- Mycobacterium research and diagnosis
- Immune cells in cancer
- Cancer and Skin Lesions
- Breast Lesions and Carcinomas
- Infection Control in Healthcare
- Psychological Testing and Assessment
- T-cell and B-cell Immunology
- Mast cells and histamine
- Educational Research and Pedagogy
- Fermentation and Sensory Analysis
- Cancer, Hypoxia, and Metabolism
- Protein Kinase Regulation and GTPase Signaling
Singapore Immunology Network
2018-2024
Agency for Science, Technology and Research
2019-2024
The University of Adelaide
2021
Konkuk University Medical Center
2018
University of North Carolina at Chapel Hill
2013
Columbia University
2003
The University of Texas MD Anderson Cancer Center
2002
Abstract Patients with type 2 diabetes (T2D) have a lower risk of Mycobacterium tuberculosis infection, progression from infection to (TB) disease, TB morality and recurrence, when being treated metformin. However, detailed mechanistic understanding these protective effects is lacking. Here, we use mass cytometry show that metformin treatment expands population memory-like antigen-inexperienced CD8 + CXCR3 T cells in naive mice, healthy individuals patients T2D. Metformin-educated increased...
BACKGROUND Sentinel lymph node examination in patients with breast carcinoma has been gaining popularity. Currently, there is no standard intraoperative assessment of sentinel nodes. To assess the utility an touch imprint (TI) evaluation, authors compared TI cytology surface hematoxylin and eosin (H&E) histology nodes from carcinoma. METHODS Sixty five biopsy cases were identified. Diagnoses H&E histologic sections compared. RESULTS Touch had a specificity 100%, negative predictive value...
Host cell chromatin changes are thought to play an important role in the pathogenesis of infectious diseases. Here we describe a histone acetylome-wide association study (HAWAS) disease, on basis genome-wide H3K27 acetylation profiling peripheral blood granulocytes and monocytes from persons with active Mycobacterium tuberculosis (Mtb) infection healthy controls. We detected >2,000 differentially acetylated loci either type Singapore Chinese discovery cohort (n = 46), which were validated...
Abstract Background Transplantation of CD34 + hematopoietic stem and progenitor cells (HSPC) into immunodeficient mice is an established method to generate humanized harbouring a human immune system. Different sources methods for isolation have been employed by various research groups, resulting in customized models that are difficult compare. A more detailed characterization isolates needed better understanding engraftable potentially non-hematopoietic cells. Here we performed direct...
Hydrogen sulfide is a common wine fault, with rotten-egg odour, which directly related to yeast metabolism in response nitrogen and sulfur availability. In grape juice, sulfate the most abundant inorganic compound, taken up by through two high-affinity transporters, Sul1p Sul2p, low affinity transporter, Soa1p. Sulfate contributes H2 S production under limitation, being reduced via Sulfur Assimilation Pathway (SAP). Therefore, strains limited are highly desirable. We report on use of toxic...
The coronavirus disease 2019 (COVID-19) pandemic had caused a severe depletion of the worldwide supply N95 respirators. development methods to effectively decontaminate respirators while maintaining their integrity is crucial for respirator regeneration and reuse. In this study, we systematically evaluated five decontamination using vaporized hydrogen peroxide (VHP) or ultraviolet (254 nm wavelength, UVC) radiation. Through testing bioburden, filtration, fluid resistance, fit (shape)...
<title>Abstract</title> Background Transplantation of CD34<sup>+</sup> hematopoietic stem and progenitor cells (HSPC) into immunodeficient mice is an established method to generate humanized harbouring a human immune system. Different sources methods for isolation have been employed by various research groups, resulting in customized models that are difficult compare. A more detailed characterization isolates needed better understanding engraftable potentially non-hematopoietic cells. Here...
The premutation of the fragile X messenger ribonucleoprotein 1 (FMR1) gene is characterized by an expansion CGG trinucleotide repeats (55 to 200 CGGs) in 5&#039; untranslated region, and increased levels FMR1 mRNA. Molecular mechanisms leading premutation-associated conditions (FXPAC) include co-transcriptional R loop formations, mRNA toxicity through both RNA gelation into nuclear foci, sequestration various re-peat-binding proteins, repeat-associated non-AUG (RAN) initiated translation...
The protective correlates of Mycobacterium tuberculosis ( Mtb ) infection-elicited host immune responses are incompletely understood. Here, we report pro-pathogenic crosstalk involving Ly6G + granulocytes (Ly6G Gra), IL-17 and COX2. We show that in the lungs -infected wildtype mice, either BCG-vaccinated or not, most intracellular bacilli Gra-resident four weeks post-infection onwards. In genetically susceptible IFNγ −/− excessive Gra infiltration with severe bacteraemia. Neutralizing...
The protective correlates of Mycobacterium tuberculosis ( Mtb ) infection-elicited host immune responses are incompletely understood. Here, we report pro-pathogenic crosstalk involving Ly6G + granulocytes (Ly6G Gra), IL-17 and COX2. We show that in the lungs -infected wildtype mice, either BCG-vaccinated or not, most intracellular bacilli Gra-resident four weeks post-infection onwards. In genetically susceptible IFNγ −/− excessive Gra infiltration with severe bacteraemia. Neutralizing...
Abstract Host-cell chromatin changes are thought to play an important role in the pathogenesis of infectious diseases. Here, we describe first histone acetylome-wide association study (HAWAS) disease, based on genome-wide H3K27 acetylation profiling peripheral granulocytes and monocytes from subjects with active Mycobacterium tuberculosis ( Mtb ) infection healthy controls. We detected >2,000 differentially acetylated loci either cell type a Chinese discovery cohort, which were validated...
ABSTRACT Methionine biosynthetic pathway, essential for the growth of Mycobacterium tuberculosis ( Mtb ) in host, represents an attractive target development novel anti-tuberculars. Here, we have biochemically characterized homoserine acetyl transferase (HSAT viz. MetA) , which catalyses first committed step methionine and S-adenosylmethionine (SAM) biosynthesis. High-throughput screening a 2300 compound library resulted identification thiram, anti-fungal organosulfur compound, as most...
Exploring intercultural communication themes in English classes through the movie Outsourced.STEM Journal
Abstract Diabetic patients taking metformin have lower risk for Mycobacterium tuberculosis ( Mtb ) infection, progression from infection to (TB) disease, TB morality and recurrence. However, a detailed mechanistic understanding of metformin’s protective immunological benefits on host resistance is lacking. In this study, using mass cytometry we show that treatment expands memory-like antigen-inexperienced CD8 + CXCR3 T cells in naïve mice, healthy diabetic humans. Metformin-educated...
Methionine biosynthetic pathway, essential for the growth of Mycobacterium tuberculosis (Mtb) in host, represents an attractive target development novel anti-tuberculars. Here, we have biochemically characterized homoserine acetyl transferase (HSAT viz. MetA) Mtb, which catalyses first committed step methionine and S-adenosylmethionine (SAM) biosynthesis. High-throughput screening a 2300 compound library resulted identification thiram, anti-fungal organosulfur compound, as most potent MetA...
Abstract The protective correlates of Mycobacterium tuberculosis ( Mtb ) infection-elicited host immune responses are incompletely understood. Here, we report pro-pathogenic crosstalk involving Ly6G + granulocytes (Ly6G Gra), IL-17 and COX2. We show that in the lungs -infected wildtype mice, either BCG-vaccinated or not, most intracellular bacilli Gra-resident four weeks post-infection onwards. In genetically susceptible IFNγ −/− excessive Gra infiltration with severe bacteraemia....