A5034185369- Immune Cell Function and Interaction
- Acute Myeloid Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Blood groups and transfusion
- Immunotherapy and Immune Responses
- Erythrocyte Function and Pathophysiology
- Hematological disorders and diagnostics
- Platelet Disorders and Treatments
- T-cell and B-cell Immunology
- Immune cells in cancer
- Immunodeficiency and Autoimmune Disorders
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Blood disorders and treatments
- Bone and Joint Diseases
- Cancer-related molecular mechanisms research
- Chronic Lymphocytic Leukemia Research
- Complement system in diseases
- Multiple Myeloma Research and Treatments
- Advanced Sensor and Energy Harvesting Materials
- Immune Response and Inflammation
- Mesenchymal stem cell research
- Cytokine Signaling Pathways and Interactions
- Reproductive System and Pregnancy
- MicroRNA in disease regulation
- RNA modifications and cancer
Tianjin Medical University General Hospital
2016-2025
Tianjin Medical University
2014-2024
China Resources (China)
2021-2024
Weatherford College
2020
University of Macau
2020
Guangdong University of Technology
2017-2019
National Natural Science Foundation of China
2013-2019
Creative Commons
2019
Guangzhou University
2014
Tianjin Medical University Cancer Institute and Hospital
2013
Myelodysplastic syndromes (MDS) are age-dependent stem cell malignancies that share biological features of activated adaptive immune response and ineffective hematopoiesis. Here we report myeloid-derived suppressor cells (MDSC), which classically linked to immunosuppression, inflammation, cancer, were markedly expanded in the bone marrow MDS patients played a pathogenetic role development These clonally distinct MDSC overproduce hematopoietic suppressive cytokines function as potent...
Abstract As a large number of strain sensors are put into practical use, their stability should be considered, especially in harsh environments containing water or microorganisms, which could affect sensing. Herein, novel strategy to overcome liquid interference is proposed. The sensor constructed with sandwich architecture through layer‐by‐layer (LBL) spray‐coating 3‐(aminopropyl)triethoxysilane (APTES) bonding layer and multi‐walled carbon nanotubes/graphene (MWCNT/G) conductive layers on...
The aberrant function of lymphocytes is considered a significant contributing factor to pure red cell aplasia (PRCA), but the precise mechanism by which T induce erythroid development stagnation remains unclear. In our study, CD8+ were isolated from bone marrow aspirates acquired PRCA patients and healthy controls. RNA sequencing (RNA-Seq) was performed analyze gene expression profiles. Additionally, levels key molecules transcription factors assessed at protein levels. RNA-Seq analysis...
The aim of the present study was to investigate number and function CD8+HLA-DR+ cells, which are considered be activated cytotoxic T lymphocytes (CTLs), in peripheral blood further examine pathogenesis severe aplastic anemia (SAA). Thirty-eight patients with SAA were included study. Patients screened for paroxysmal nocturnal hemoglobinuria by flow cytometry using anti-CD55 anti-CD59 antibodies. cells measured three-color anti-CD8-peridinin chlorophyll, anti-CD3-fluorescein isothiocyanate...
Myelofibrosis (MF) is a bone marrow disorder characterized by clonal myeloproliferation, aberrant cytokine production, extramedullary hematopoiesis, and fibrosis. Although somatic mutations in JAK2, MPL, CALR have been identified the pathogenesis of these diseases, inhibitors Jak2 pathway not demonstrated efficacy ameliorating MF patients. TGF-β family members are profibrotic cytokines we observed significant TGF-β1 isoform overexpression large cohort primary patient samples. Significant was...
Severe aplastic anemia (SAA) is a rare disease characterized by severe pancytopenia and bone marrow failure. Natural killer (NK) cells are large granular lymphocytes derived from hematopoietic stem (HSCs) or common lymphoid progenitors (CLP). They play key role in n the innate immunity adaptive immune. In this study, quantitative functional changes of natural cell subsets peripheral blood patients before after immunosuppressive therapy (IST) were investigated. Results showed that percentage...
Ferroptosis is a form of iron-dependent non-apoptotic cell death, with characteristics loss the activity lipid repair enzyme, glutathione (GSH) peroxidase 4 (GPX4), and accumulation lethal reactive oxygen species. The mechanism ferroptosis in myelodysplastic syndrome (MDS) unclear.Cell viability assay, species (ROS) GSH GPX assay were performed to study regulation MDS cells obtained from patients, iron overload model mice, lines.The growth-inhibitory effect decitabine could be partially...
In this work, a novel flexible electrically resistive-type MXene/Thermoplastic polyurethanes(TPU) based strain sensors was developed by composite process of electrospinning (ES) and electrostatic spray deposition (ESD). Compared with other processes, the sensing layer prepared ESD has better adhesion to ES TPU nanofiber membrane is not easy crack during stretching process, thereby greatly improving working range sensor. Furthermore, we obtained sandwich structure easily on surface again....
ABSTRACT Single‐cell sequencing of lineage negative (Lin‐) cells from patients with myelodysplastic syndromes (MDS) revealed a reduction in ferritin heavy chain 1 ( FTH1 ) levels, yet the significance this decrease pathophysiology MDS remains unclear. In study, we evaluated role MDS. The mRNA expression GlycoA + nucleated erythrocytes was significantly lower than that control group. implicated both ferritinophagy and ferroptosis patients, processes are linked to development anaemia. To...
To investigate the effect of iron overload on expression programmed death-1 (PD-1) surface T lymphocyte in mice, order to analyze mechanism inhibiting cell function. Flow cytometry was used detect labile pool (LIP), reactive oxygen species (ROS), and PD-1 peripheral blood cells mice with overload. The mean fluorescence intensity calcein group 2 492±311.1, which significantly lower than 3 136±537.3 control ( P <0.01), suggesting that increased LIP group. Compared group, ratio CD4/CD8 normal...
ABSTRACT Background Thymoma‐associated pure red cell aplasia (PRCA) is a rare autoimmune disorder characterized by selective erythroid lineage suppression. However, the underlying immune mechanisms remain unclear. Methods We performed single‐cell RNA sequencing (scRNA‐seq) on bone marrow cells from thymoma‐PRCA patients and healthy controls to analyze hematopoietic populations. Additionally, we conducted bulk of peripheral blood CD8 + T cells, flow cytometry analysis T‐cell activation,...
Myelodysplastic neoplasm (MDS) patients are at a high risk of infections, contributing significantly to morbidity and mortality. While neutrophil dysfunction is considered primary factor, specific functional defects remain elusive. We conducted comprehensive study involving 90 participants, including controls de novo MDS patients. utilized the TAXIScan-FL system evaluate chemotaxis towards leukotriene B4 (LTB4). The global reactive oxygen species (ROS) production by neutrophils were measured...
ABSTRACT Objective VEXAS (Vacuoles, E1 enzyme, X-linked, Autoinflammatory, Somatic) syndrome is an identified haemato-rheumatoid disease caused by somatic UBA1 mutations in hematopoietic stem cells. We report Uba1 loss various mouse cell types leads to diverse effects, with approximately 70% depletion neutrophils inducing non-lethal VEXAS-like symptoms. Methods Using nine different Cre/flox-mediated conditional-knockout (CKO) models, we interrogated the phenotypes of . Neutrophil-specific...
Hepatitis-associated aplastic anemia (HAAA) is a variant of severe (SAA) in which bone marrow failure follows an acute attack hepatitis. Its pathogenesis poorly understood. We investigated the prevalence HAAA among cases newly diagnosed SAA presenting to our hospital between January 1998 and February 2013, analyzed clinical immune characteristics non-hepatitis-associated (non-HASAA) patients. The was 3.8% (36/949), majority patients (33/36) were seronegative for known hepatitis virus....
Multiple myeloma is a hematologic malignancy characterized by the accumulation of monoclonal plasma cells in bone marrow. A common manifestation disease (MBD), which caused increased osteoclastic resorption and decreased formation. The chemokine cytokine ligand 3 (CCL3) pro-inflammatory protein that stimulates osteoclasts MBD. However, little known about effect CCL3 on osteoblasts (OB). OBs are induced from patients with MBD healthy donors, cultured vitro, identified histochemistry. effects...
Myelodysplastic syndromes (MDS) represent a heterogeneous group of clonal hematopoietic disorders characterized by ineffective haematopoiesis, refractory cytopenia, and an increased risk progression to acute myeloid leukaemia. This study investigates the presence cellular senescence in bone marrow (BM) CD235a+ erythrocyte precursors MDS patients explores its correlation with anaemia. We assessed senescence-related markers cell cycle distribution BM patients. Correlation analyses were...